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Methods: The three lung cancer cell lines-A549(highly invasive human lung adenocarcinoma cell ), 95-D(highly metastatic human lung carcinoma cell), and SPC-A-1(lowly metastatic human lung adenocarcinoma cell) were cultured conventionally in vitro. When the cells covered the bottom of culture flask, we abstracted the protein with lysis solution containing RIPA and PMSF, and obtained RNA with RNeasy Mini Kit.

将三株肺癌细胞：高侵袭的肺腺癌细胞(A549)、高转移的肺癌细胞(95-D)、低转移的肺腺癌细胞(SPC-A-1)做常规细胞培养，待细胞长满瓶底后，用RIPA和PMSF组成的细胞裂解液提取细胞蛋白质，RNeasy Mini Ki提取RNA。

A highly active hydrogen added catalytic nanometer Ru/C is made by reducing metal ruthenate, getting nanometer ruthenate metal glue under the protection of surface activator, filtering, washing glue solution drawing by the carrier to get highly scattered loading nanometer ruthenate metal catalyst.

本发明涉及一种高活性加氢催化剂纳米Ru/C的制备方法，该方法首先通过化学还原金属钌盐，制得表面活性剂保护下的纳米钌金属胶体，然后用载体吸附制得的胶体溶液，经过滤，洗涤，获得高度分散的负载型纳米钌金属催化剂。

Factory lies in dongfeng town, zhongshan city, guangdong province, with a complete set of high technological production equipments and strict test procedures, which ensure high quality of all the products. our products are exported to europe, america, mideast, asia and africa,etc.highly skil LED technologists and capable salesmen have produced one-line service, includingresearch,production and sale, which has been highly a f IR med by our customers.

工厂设在广东省中山市东凤镇，成熟配套的高精密生产设备和严格的检测工序，保证了高品质的产品出厂，产品遍及欧美、中东、亚洲及非洲等地，技艺纯熟的专业技术人员和高素质的销售人员形成了集研发、生产到销售的一站式服务，得到了客户的一致好评和肯定。

Work 工作:(1) feasible process development for high-pure tetraalkyphosphonium salt as catalysts for polymerizations, potential sale 2-4 million us$;(2) simple and feasible preparation process for phosphonium salt as ionic liquids, biocides and phase transfer catalysts;(3) new and industrially feasible processes for highly sterically hindered alkylphosphines as catalyst ligands, these include tricyclohexyl-phosphine, phosphorinanes and dsmphos2,3- bis(9-phosphabicyclononylbutane;(4) sterically hindered dialkylphosphinic acid as new generation of co/ni extractant;(5) alkylphosphonium wittig reagents and the wittig reactions with them;(6) exploration of the application of borane chemistry in the synthesis of highly sterocally hindered organophosphines;(7) customer preparation of samples of a variety of organophosphorus compounds for customers all over the world.

用作高分子聚合反应催化剂的高纯度四烷基瞵盐的实用生产流程。产品销售前景 2-4 百万美元；(2)用作离子化液体，杀菌剂和相转移催化剂的烷基瞵盐的简单实用合成方法；(3)高位阻烷基磷化氢催化剂配体的工业实用合成路线的研究，这包括，三环己烷基磷， phosphorinanes 和 dsmphos 2，3-bis(9-phosphabicyclononylbutane ；(4)高位阻二烷基膦酸金属萃取剂的工业实用合成路线的研究；(5)烷基膦 wittig 反应试剂的研究；(6)硼化学在高位阻烷基磷化氢合成中的应用；(7)为世界各地提供顾客合成服务。

To this end,we used the Transwell system to select two highly invasive subcell lines SNU5-P3 and PAMC82-P3 from minimally invasive parent cells,and compared gene expression in paired cell lines wit...h high and low invasive potentials.38 genes were commonly upregulated in the two highly invasive subcell lines.

通过全基因组芯片比较SNU5-P3和PAMC82-P3与其亲本细胞的基因表达谱，获得了38个在两张芯片共同上调表达的差异基因，其中16/38(42%)已报道是与侵袭转移功能相关的基因。LOXL2是一个在两张芯片共同上调表达的基因，通过生物信息学分析和细胞表达谱分析，表明LOXL2可能是胃癌侵袭转移密切相关的功能基因。

Results At the dosage of 25 mg/kg,AN could induce higher incidence of fetal resorption and death,and decrease the average fetal body weight and le ngth with significant difference(P＜0.05)when compared with neg ative controls.At the dosage of 35 mg/kg,AN could decrease average vital fet us p er litter with significant difference(P＜0.05),and induce chang es of other indexes with highly significant difference(P＜0.0 1).At the dosage of 25 and 35 mg/kg,AN could induce higher teras rate,maternal t eras rate and malformation rate of vital fetus with significant and/or highly s ignificant difference(P＜0.05 or P＜0.01),resp ectively.There existed a dose-effect or dose-response relationship between abo ve indexes and doses.

结果 25 mg·kg-1组死胎率、吸收胎率升高，胎鼠平均体重、体长、尾长减小，与阴性对照组比较差异有显著性(P＜0.05)；35 mg·kg-1组除窝平均活胎数减少，差异有显著性( P＜0.05)，其余各指标与阴性对照组比较差异有非常显著性(P ＜0.01)；25，35 mg·kg-1组畸胎率、母体畸胎率、活胎畸形率升高，与对照组相比差异有显著性和/或非常显著性(P＜0.05或P ＜0.01)，且有明确的剂量效应或剂量反应关系。

Moreover, he possessed what is, for a highly strung and highly intelligent man, an even more remarkable accomplishment: he knew how to do nothing.

他生的火总是燃烧得很旺的。他洗擦过的地板，干净得一尘不染。

This highly essentialized re-imaging of Chinese history explicitly bifurcates "Chineseness" into a sedentary/Han/sheep versus nomadic/Mongol/wolf dyad, rendering any notion of shared national identity highly problematic.

这个高度本质化重新明确中国历史的成像分叉&的华人&成为定居/韩/羊与游牧/蒙古/狼来了二分，使任何国家认同的共同的概念很成问题。

This highly essentialized re-imaging of Chinese history explicitly bifurcates "Chineseness" into a sedentary/Han/sheep versus nomadic/Mongol/wolf dyad, rendering any notion of shared national identity highly problematic.

这种对中国历史高度简约性的描述，明确地把&中华民族&分成了两类并加以对比：定居/汉族/羊来与游牧/蒙古/狼。这种泾渭分明的划分对所有民族认同都都提出质疑。。

This mechanism has the benefit of allowing high levels of Cx while remaining highly sensitive to small changes in charge coupling due to touch; the circuit permits the designer to create very large, highly interdigitated touch keys that are very sensitive.

这种机制允许有高水平的Cx的利益，同时保持高度敏感，负责耦合的微小变化，由于接触，电路允许设计人员创建非常大的，高度叉触摸键非常敏感。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了