查询词典 highly-academic

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The company specialized productionenrichment plant, provides the technical process,the dressing experiment,the installment instructswww.lzkysb.cn gently yu0797-5745599 to stir the equipment, the stirring chest, the highly effective stirring chest, the leaching vat, the promotion stirring chest, the mineral product stirring chest, the mineral product promotion stirring chest, the mixer, the mineral product mixer, the highly effective mixer, promotes the mixer, stirs the tube, stirs the barrel, the mineral product agitation barrel, the promotion agitation barrel, the stirring chest which the company produces, the promotion type agitation barrel, the highly effective stirring chest, the bilobed wheel stirring chest, the leaching vat mainly uses in the density not being bigger than 30%the proportion is not bigger than 3.5 pulpthe flotation reagents for ore-dressing carries on the agitation, this product divides XBthe XBT promotion type agitation barrel, the XBT promotion type agitation barrel in carries on to the pulpAgitation at the same time may promote the pulp liquid level to certain altitude, for this reason the promotion type agitation barrel to does not have the dropping variance dressing location to be extremely advantageous mainly by the trough body, the support, the electrical machinery, the impellerthe drive shaftso on is composed.

公司专业生产选矿设备，提供工艺流程，选矿实验，安装指导www.lzkysb.cn温良禹0797-5745599搅拌设备，搅拌槽，高效搅拌槽，浸出槽，提升搅拌槽，矿用搅拌槽，矿用提升搅拌槽，搅拌机，矿用搅拌机，高效搅拌机，提升搅拌机，搅拌筒，搅拌桶，矿用搅拌桶，提升搅拌桶，公司生产的搅拌槽，提升式搅拌桶，高效搅拌槽，双叶轮搅拌槽，浸出槽主要用于浓度不大于30%和比重不大于3.5的矿浆与浮选药剂进行搅拌，该产品分XB和XBT提升式搅拌桶， XBT提升式搅拌桶在对矿浆进行搅拌的同时可将矿浆液面提升到一定的高度，为此提升式搅拌桶对没有落差的选矿场地极为有利主要由槽体，支架，电机，叶轮及传动轴等组成。

The company specialized productionenrichment plant, provides the technical process,the dressing experiment,the installment instructswww.lzkysb.cn gently yu0797-5745599 to stir the equipment, the stirring chest, the highly effective stirring chest, the leaching vat, the promotion stirring chest, the mineral product stirring chest, the mineral product promotion stirring chest, the mixer, the mineral product mixer, the highly effective mixer, promotes the mixer, stirs the tube, stirs the barrel, the mineral product agitation barrel, the promotion agitation barrel, the stirring chest which the company produces, the promotion type agitation barrel, the highly effective stirring chest, the bilobed wheel stirring chest, the leaching vat mainly uses in the density not being bigger than 30% and the proportion is not bigger than 3.5 pulp and the flotation reagents for ore-dressing carries on the agitation, this product divides XB and the XBT promotion type agitation barrel, the XBT promotion type agitation barrel in carries on to the pulpAgitation at the same time may promote the pulp liquid level to certain altitude, for this reason the promotion type agitation barrel to does not have the dropping variance dressing location to be extremely advantageous mainly by the trough body, the support, the electrical machinery, the impeller and the drive shaft and so on is composed.

公司专业生产选矿设备，提供工艺流程，选矿实验，安装指导 www.lzkysb.cn 温良禹0797-5745599搅拌设备，搅拌槽，高效搅拌槽，浸出槽，提升搅拌槽，矿用搅拌槽，矿用提升搅拌槽，搅拌机，矿用搅拌机，高效搅拌机，提升搅拌机，搅拌筒，搅拌桶，矿用搅拌桶，提升搅拌桶，公司生产的搅拌槽，提升式搅拌桶，高效搅拌槽，双叶轮搅拌槽，浸出槽主要用于浓度不大于30%和比重不大于3.5的矿浆与浮选药剂进行搅拌，该产品分XB和XBT提升式搅拌桶， XBT提升式搅拌桶在对矿浆进行搅拌的同时可将矿浆液面提升到一定的高度，为此提升式搅拌桶对没有落差的选矿场地极为有利主要由槽体，支架，电机，叶轮及传动轴等组成。

The chapter two was focused on the samarium diiodide promoted organic reactions and their applications in organic synthesis, firstly, a new synthesis of highly substituted cyclopentadienes from a,β-alkynone promoted by samarium diiodide was investigated and the highly substituted cyclopentadienes were prepared in moderate to good yields under mild conditions. Secondly, the Michael addition and Michael-aldol tandem reaction of diorganyl diselenides or diorganyl disulfides with α,β-alkynones mediated by samarium diiodide were studied and a series of alkenylsulfides and alkenylselenides were prepared in good yields. Then the highly regioselecfive nucleophilic addition of the allylsamarium bromide to α,β-alkynones were explored. We found that the allylsamarium bromide reacts with α,β-alkynones to afford regiospecificly 1. 2-nucleophilic addition products in good yields under mild condition. At last we studied the coupling reaction of aryl halides promoted by NiCl〓/PPh〓/Sm〓.

第二章 研究了二碘化钐、金属钐促进的有机反应：1、二碘化钐促进下α，β-炔基酮的偶联环化反应，提供了一种制备多取代环戊二烯的新方法；2、二碘化钐促进下的二硫醚、二硒醚与α，β-炔基酮的Michael加成反应，为高产率地制备多取代的烯基硫或烯基硒化合物提供了方便可靠的新方法；3、二碘化钐促进下的二硫醚、二硒醚与α，β-炔基酮和醛、酮的Michael—aldol串联反应，提供了一种一锅法高产率地制备多取代的含硫的烯丙基醇或含硒的烯丙基醇类化合物的方便可靠的原子经济性的新方法；4、烯丙基溴化钐与α，β-炔基酮的高度区域选择性的1，2-亲核加成反应；5、NiCl〓/PPh〓/Sm〓促进的卤代芳烃的偶联反应-一种从各种卤代芳烃制备联苯类化合物的有效方法。

It can be recognized deeply for the essence of academic freedom from the visual angle of academic self-discipline.

从学术自律的角度出发，可对学术自由的本质得到更加深刻的认识和解读。

According to different academic freedom activities, there are different scope and standards of academic freedom.

最后从政治、法律、经济和道德等方面探讨了学术自由限度的制约因素。

In modern society, academic freedom is not just an academic values, but also the basic str...

在现代社会，学术自由不仅仅是一种学术价值观，更是现代大学的基本组织制度。

Yale is above all an academic institution, and thus academic strength is our first consideration in evaluating any candidate.

耶鲁首先是一个学术机构，因此申请人的学术能力是我们的第一考虑。

The following example creates a query that returns the name and rank of seniors whose academic rank is between 1 and 10, in order of academic rank.

下面的示例创建一个查询，返回学习名次位于1和10之间的高年级学生的姓名和学习名次，并按学习名次排序。

He has a senior academic rank or a doctoral degree, or has a recommendation from two science and technology persons who are working in the same research field and have a senior academic rank.

具有高级专业技术职务或者具有博士学位，或者有2名与其研究领域相同、具有高级专业技术职务的科学技术人员推荐。

Methods Clinical data of 3 cases of bile duct injury during laparoscopic cholecystectomy were analyzed retrospectively.

回顾总结3例LC术中胆管损伤的临床资料，分析3例胆管损伤的原因及对策。

These techniques are applied to the gobang.

本文将这些技术用于五子棋中。