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The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

The company with the highest market share is likely to have the highest profit margin.

公司有最高的市场份额可能有最高的利润率。

Results: Relative diagnostic accordance rate of the clinical exploration and four kinds of image processing setup was the highest（P＞0.01）in the first premolars. Relative diagnostic accordance rate of the clinical exploration and the pseudocolor image setup and negative image setup was the highest（P＞0.01）, and that of the clinical exploration and the pseudo3D image setup and positive image setup was the lowest（P＜ 0.01）, showing statistical differences in the second premolars.

结果：①第一前磨牙：4种图像处理格局下邻面龋的检出结果与临床诊断结果的符合率高，差异无统计学意义（P＞0.01）；②第二前磨牙：负片和伪彩图像格局下邻面龋的检出结果与临床诊断结果的符合率高，差异无统计学意义（P＞0.01）；③正片和伪3D图像格局检出结果和临床诊断结果的符合率低（P＜0.01）。

The highest mountain peak is Qomolangma, the highest in the world, 8,848 meters above sea level; the lowest point is the Turpan Basin, 154 meters below sea level.

最高的山峰是珠穆朗玛峰，是世界上最高的，八千八百四十八米高于海平面，最低点是吐鲁番盆地，低于海平面154米。

More children with headaches were in the highest quintile of risk for these factors, as well as in the highest quintile of risk of having at least 3 or more of these factors.

更多有头痛的儿童，他们这些因子的风险处於最高的四分位数，且有3种以上这些因子的风险也是最高的。

Among women with benign breast disease, a non-significant positive association with breast cancer risk was observed for those in the highest quintile of caffeine consumption and a significant association was observed for those in the highest category of coffee consumption .

在妇女良性乳腺疾病，非重大的积极与患乳腺癌的风险，观察这些在最高的五分之一的咖啡因消费和重大关联有人为那些在最高等级的咖啡消费量。

By using testing functions to make comparative research on the precision of approximate models which were built by RBF under the condition of different sample amounts and different kernel function types, a conclusion was drawn: the model quality built by Reciprocal Multiquadric RBF as kernel function was highest when the sample amout was big, and the model quality built by Gaussian RBF as kernel function was highest when the sample amount was small.

运用测试函数对在不同样本数量与不同核函数形式下所建立的近似模型的精度进行对比研究，得出逆多二次函数与高斯函数作为核函数分别在大样本和小样本情况下精度最高的结论，并验证了径向基函数近似模型方法能够兼顾效率与精度。

Contents of salidroside and tyrosol in Rhodiola roots were determined. Salidroside content was 2.147%, the highest in Rh. sacra from Tibet, then in Rh. crenulata and Rh. tibetica from Tibet, 1.763% and 1.271%, respectively; tyrosol content was 0.2985%, the highest in Rh. crenulata from Sichuan, then in Rh. tibetica and Rh. crenulata from Tibet, 0.2143% and 0.1836%, respectively; whereas both contents were very low in Rh. jistigiata and Rh. kirilowii from Sichuan with salidroside contents of only 0.034% and 0.003%, and tyrosol contents of 0.0164% and 0.0048%,respectively.

分别测定了不同产地的红景天属植物中红景天苷和酪醇的含量，西藏产圣地红景天中红景天苷含量最高，达到2.147%，其次为藏产大花红景天和西藏红景天，分别为1.763%和1.271%；四川产大花红景天中酪醇含量最高，为0.2985%，其次是藏产西藏红景天和大花红景天，分别为0.2143%和0.1836%；长鞭红景天和狭叶红景天中2种有效成分的含量均很低，红景天苷含量仅为0.034%和0.003%，酪醇含量则分别为0.0164%和0.0048%。

And has demonstrated humanity's self-sovereignty will, to the highest pursuit of social power,but also for the pursue world progressive young people, set up the human self-sovereignty will highest idol standard, life style,.

又展示了人类的自我主权意志，对社会权力的最高追求，还为追求世界进步的青年人，树立起了人类自我主权意志的最高偶像的标准，生活的风格

Now you have learned to their own capability to own a suitable set of the highest credit card limit per month, the highest limits in the credit card, you are safe.

现在你学会了自己量力而行，给自己定了一个适合自己情况的每月刷卡最高限，在这个最高限内刷卡，你是安全的。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了