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DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

On the other hand, mice primed with all the microsphere formulations showed higher anamnestic responses than those primed with soluble tetanus toxoid. Higher anamnestic response means stronger protection from infection.

另一方面，用微球免疫过的小鼠再次接受低剂量抗原时，所产生的免疫记忆抗体高于用破伤风类毒素溶液免疫过的小鼠，说明微球具有更强的免疫保护力。

The data showed that the capability of hydrolysis of alcalase was higher than that of protamex; peanut peptides have obvious antioxygenic property and ability to clear HO in effect and increased with a higher degree of hydrolysis.

结果表明，ALCALASE对花生蛋白的水解能力优于PROTAMEX；花生肽具有较强的抗氧化性及有效清除羟自由基的能力，在本试验范围内随水解度的增加而提高。

Shade on the flower bolting stage can obvious improved the SPAD value in leaf and appreciatory value. The shading degree was higher, the soluble sugar and starch content in leaf was lower. But the flower formation was not affected because it may still higher than critical value.

3在花茎抽生期，对大百合进行遮荫处理能提高植株株型的观赏价值，遮荫程度越高，其叶片的SPAD值越高，叶片数越多和叶面积越大，同时株高表现也尤为显著。

Arbuscule colonization of FenGe was significantly higher than that of YaoGe, spore density of YaoGe was 7.95 fungi/g soil, significantly higher than that of CaiGe and FenGe.

丛枝定殖率粉葛显著高于药葛；孢子密度药葛高达7.95个/g土，显著高于菜葛和粉葛。

It was higher than those of Schima and Psychotria by 8.8% and 10.7%, and higher than that of Ardisia by 14.7%, respectively.

疏林桃金娘的不包括光呼吸的CO2补偿点为Γ*38.1 μL L-1，较荷木和九节分别约高8.8％和10.7％；较罗伞约高14.7％。

ER is significantly higher in the basal layer, outer root sheath, sebaceous gland, eccrine gland and arrector pili muscle in the female patients. PR in arrector pili muscle of male patients and in the basal layer, eccrine gland of the female patients is significantly higher.

孕激素受体brogesterone receptof3 PRPR在工毛二中文摘要肌处男性患者阳性表达明显高于正常对照0＜0.of）；在基底层及汗腺女性患者阳性表达明显高于正常对照 0功。05）；余部位比较在统计学上无显著差异。

2The fatty liver patients have higher risk of cardiovascular disease, hypertension, diabetes mellitus and have higher arteriorenal resist index than others.

2脂肪肝人群中冠心病、高血压病、糖尿病的患病率及肾动脉阻力指数显著高于非脂肪肝人群。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

Higher vocational education requires students majoring in electric automa- tion to be equipped with higher comprehensive quality and practical ability in automa- tion technology.

高等职业教育是中国高等教育的重要组成部分，是工业化和生产社会化、现代化的重要支柱。

Haihe River by the King Bridge to Taku, 73 kilometers long.

海河干流由金刚桥到大沽口，长73公里。

Thermoset Molding; beads granular sodium hydroxide; potassium hydroxide; massive six water ferric chloride; copper; sodium metasilicate

秉持着诚信、专业的经营理念，为客户提供产品专业知识及技术支持，热诚服务。