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However, the 3 groups from medium A exhibited the highest microbial diversity and best decolorization results with 99.53% and 97.42% color removal rate of Reactive Red M-3BE and Acid Red. From them, 16 strains of fungi were isolated and primarily identified as Saprolegniaceae, Eurotiaceae, Erysiphaceae and Physodermataceae . Fungi groups from medium B and D exhibited a bit lower color removal rate of various dyes and only 3 and 2 isolates primarily classified as Saccharomycetaceae and Eurotiaceae were obtained from them. Fungi cultures in medium A and B could produce lignin peroxidase, and those in medium D could be detected higher activity of laccase. All the fungal cultures exhibited very weak activity of manganese dependant peroxidase.

来自A培养基的3组菌群显示出最好的脱色效果和最大的菌群丰富度,对50mg/L的活性红M-3BE和酸性红A溶液的脱色率最高达到99.53%和97.42%,从中分离到了16株真菌,初步鉴定分属于水霉科、曲霉科、节壶菌科和白粉菌科;而B和D培养基中所获得的菌群脱色效果稍差,从中仅得到3株和2株真菌,初步鉴定属于酵母和青霉。A、B两种培养基在各种染料存在下更易产生木质素过氧化物酶,产漆酶能力较弱,而D培养基产漆酶活性较高。

2There are differences between China's best young football coaches in terms of age, seniority, education, marriage, coaches level, nature of work, the nature of work units, regions, eat. In which older and younger age with higher satisfaction, which is into "U" type ;In seniority, the lower academic qualifications, the higher job satisfaction, showing the antigrowth trend. Level in the coaches, the higher the level on the career identity and career development with lower satisfaction, and other dimensioned showed positive growth. In marriage, unmarried outstanding youth football coaches have higher satisfaction in career identity dimensions. In the nature of the work, the coaches satisfaction is higher, but the degree of salary is lower. Nature in the workplace, the youth football coaches in large-scale enterprises outstanding football club are with higher satisfaction. At the district level, the developed coastal regions are with higher job satisfaction, the old industrial base in the northeast have lower job satisfaction in the work environment and salary degrees. regard to remuneration, salaries in 2000-3000or more than 5000 outstanding young football coaches have higher job satisfaction in the current, but salaries in 3000-4000 or 4000-5000 outstanding football coaches, the job satisfaction is slightly lower三、There is significant positive correlation between job satisfaction and job involvement of China's best young football coaches.

其中年龄较大和年龄较小者的满意度较高,满意度成呈&U&型;在教龄方面,工作年限较少的优秀青少年足球教练员的满意度较高;在学历方面,学历越低,工作满意度越高,呈反增长趋势;在教练员级别方面,级别越高者对职业认同和职业发展的满意度较低,其他维度呈正增长;在婚姻方面,未婚的优秀青少年足球教练员有较高的工作满意度,但已婚优秀青少年足球教练员对职业认同维度有较高的满意度;在工作性质方面,主教练的的满意度较高,但在薪资福利维度上的满意度较低;在工作单位性质方面,大型企业足球俱乐部的优秀青少年足球教练员拥有较高的满意度;在地区方面,沿海发达地区的工作满意度水平较高,东北老工业基地的工作满意度水平在工作环境和薪资福利维度的满意度水平较低;在薪酬方面,薪酬在2000-3000和5000以上的优秀青少年足球教练员对目前的工作满意度较高,而薪酬在3000-4000和4000-5000的优秀青少年足球教练员的工作满意度略低。

During the process of the detailed argument, the author of this paper, by giving over the current situation of the financial investment system of higher education and its evolution, draws the conclusion that the seriously insufficient investment from the governmental finance and the unformed pluralism of the financial investment system of higher education are the main problems of the financial investment system of higher education in our country. Through the deep analysis of the current situation of the financial investment system of higher education in our country and the problems of it, this paper draws the conclusion that it is the imperfection of the laws for the financial investment system of higher education and the misposition of the educational management by governments that cause these problems. Based on the experiences of the western developed countries such as the USA and Japan in the financial investment system of higher education, this paper puts forward the strategy to improve it, with its focus on perfecting the laws for the financial investment system of higher education and the carrying out of the pluralism of the investment system of higher education.

在具体论述过程中,通过对高等教育财政投资体制的现状及演变过程的回顾,从而得出政府财政投入严重不足与高等教育财政多元化投资体制的内外环境尚不成熟,是我国高等教育财政投资体制的现实背景与存在的主要问题的研究结论;通过对我国高等教育财政投资体制现状与问题的深入分析,从而得出高等教育财政投入法律制度的不完善与政府教育管理职能的错位,是导致存在各种问题的主要原因的研究结论;在借鉴美国、日本等西方发达国家高等教育财政投资体制经验的基础上,提出了完善教育产业属性下高等教育财政投资体制的对策,并将论述的重点定位于完善高等教育财政投资的法律体系与实现高等教育投资体制的多元化。

Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺,真菌总数均值为3.75×10^3cfu/立方公尺,合计为5.41×10^4 cfu/立方公尺,其中真菌占6.92%;对不同采样点的空气细菌和真菌进行了初步鉴定,优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属,分别占总细菌的37.3%,21.0%,10.3%,9.5%;真菌共检出5属,其中优势菌属为曲霉属、青霉属、毛霉属、根霉属,分别占总真菌的42.0%,36.0 %,4.4%,4.2%。

On the base of the constructing phylogenetic tree using PAUP 4.0, MP and NJ (neighbor-joining) trees of diverse pectate and pectin lyases showed that pectate lyases from plant, pectin lyases from fungi and pectate lyases from bateria belong to different groups, respectively. The pectate lyase A of Aspergillus nidulans is more closely related to plant pectate lyases than to pectin lyase from fungi and pectate lyases from bacteria. The pectin lyase from Pseudomonas syringae is more closely related to fungi pectin lyases than other lyases.

构建的MP树和NJ树(neighbor-joining)显示:来自植物的果胶酸裂解酶、真菌的果胶酸酯裂解酶、细菌的果胶酸裂解酶分别可聚为一个独立的类群;相对于细菌果胶酸裂解酶和真菌果胶酸酯裂解酶而言,构巢曲霉的果胶酸裂解酶A和植物的果胶酸裂解酶之间有较近的亲缘关系;细菌Pseudomonas syringae的果胶酸酯裂解酶和真菌的果胶酸酯裂解酶之间的亲缘关系较近。

Roots of five species of Calanthe and two species of Phaius from north, midland and south of Taiwan were collected for the isolation of orchid mycorrhizal fungi. Total for 104 isolates of fungi were isolated. They are identified respectively by the traditional method or DNA sequence. Results showed thatTrichoderma and Mucor were most often isolated fungi. The DNA sequence results showed that only Xylaris was most common fungus.

由台湾北、中、南四处地方采集五种原生根节兰及二种原生鹤顶兰,进行此等原生兰花根部菌类分离,共分离出104株代码真菌菌种,分别再以菌落、菌丝和孢子等传统形态特徵鉴定菌种,结果发现以木霉菌及毛霉菌属内之菌株占优势,而利用核酸序列比对之鉴定结果则以炭角菌属内之菌株占多数。

The study reveals that Shanxi Province is rich in entomogenous fungi diversity. About 72 species of 17 genera of fungi are believed exist in the province. Among the fungi collection, 63 species are collected from four national nature reserves in the province, 12 from six major forest areas and another 22 species from the area around Taiyuan. This respectively represents 64.9%, 12.4% and 22.7% of the species collection.

结果表明,山西省虫生真菌资源十分丰富, 10年共采集分离到虫生真菌17属72种,在山西省的四大国家级自然保护区、六大林区及太原等县市分别分离到虫生真菌63种、12种和22种,分别占分离到的山西省虫生真菌的64.9%、12.4%和22.7%。

Arbuscular mycorrhizal symbiosis is the mutualistic association between AM fungi and most terrestrial plants: the plant roots offer carbohydrates to the fungi; in return, the fungi assist the plants with uptake of mineral nutrients, particularly phosphate, from soil, and their subsequent transfer to the root cortical cells.

南京农业大学资源与环境科学学院,南京 210095摘要丛枝菌根(arbuscular mycorrhizal, AM)共生是丛枝菌根真菌与大多数陆地植物的根系之间形成的一种互利共生关系。植物给菌根真菌提供碳水化合物;作为回报,菌根真菌能够增强植物对矿质营养元素的吸收。

Chitinase is a group of proteen which is expressed when plants are endanged by fungi. It can hydrolyze chitin which is main and important component of the cell wall of fungi except for Oomycetes in order to prohibit growing of fungi.

几丁质酶是植物在受到病原菌侵染时所表达出的一组蛋白质,可以水解构成真菌细胞壁的主要组成成分几丁质,从而抑制真菌的生长。

Results showed that, pyrolin had obvious action on two species of fungi, and the antifungal effect was positively correlative with concentration. The EC50 was 0.4076 mg/mL and 0.4626 mg/mL respectively after cultured 3 days. The main antifungal period was the fast growth period of fungi, and lead to it lag behind. Observation under SEM revealed that pyrolin caused a series of marked morphological and structural alterations of hyphal. These changes included mutual adhesion of mycelium, the hyphal was irregular in diameter, local denting, some of mycelium was broken, disruption of cell wall, outbreaking of inner cytoplast, and the empty cavity appeared. As a result, pyrolin restrained the growth of these fungi.

结果表明,鹿蹄草素对上述两种真菌均具有明显的抑制作用,抑菌效果与浓度呈正相关,培养3d时的EC50分别为0.4076、0.4626 mg/mL;抑菌时期主要是在真菌的快速生长期,使其快速生长期滞后;扫描电镜显示,鹿蹄草素作用下菌体形态发生异常变化,表现为菌丝体之间相互粘连,菌丝粗细不均匀,细胞壁破裂,局部凹陷,部分菌丝断裂,大量内容物外溢,出现空腔等现象,从而发挥其抑菌作用。

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