查询词典 high-water line

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The prevalence rate of NTM in Shanghai shows increased tendency.Most of NTM isolates are Group Ⅳ rapidly growing NTM.NTM shows high drug resistance to first line antituberculotic substance.

上海市NTM感染呈逐年上升趋势；感染菌种的分布以Ⅳ群快速生长的NTM为主；NTM对常用的抗结核药物呈高度耐药现象。

Line Rotary die-cutting on cigarette packs such high-end application has taken shape in rrepublic, a circle applanation process with very little rrepublic is mainly used for large scale of products.

连线圆压圆模切在烟包等高端领域的应用已经初具规模。而圆压平工艺用得很少。主要用于大尺寸产品的模切。

For example, high-speed rotary machine speed fast, hot stamping and embossing line contact, higher than the temperature will round applanation or pingya.

例如，高速圆压圆机速快，压印线接触，烫印温度就要高于圆压平或平压平。

ABB is supplying Holland America Line with high voltage shore connectionsto eliminate the emissions of ships berthed at a leading U.S. port.

ABB正在为荷兰美洲航线提供高压岸上线路解决方案帮助一个主要的美国港口减少船只在停泊处的排放。

To study the similarities and differences between male sterility induced by physiology and heredity, different male-sterile materials induced by heredity [S-type male-sterile line ms2611, ms1376] and physiology (induced by high temperature and chemical hybridizing agents SQ-1), Xinong 1376 and Xinong 2611 were used as materials, and their spikes at mononucleated prophase stage, anther and flag leaf at mononucleated anaphase stage, binucleated stage and trinucleated stage were used to conduct protein analysis.

为了研究小麦遗传型和生理型雄性不育在生化机制上的异同，以遗传型［S型不育系ms2611和ms1376］和生理型雄性不育小麦西农2611和西农1376（高温诱导和化学杀雄剂SQ-1诱导的雄性不育）为材料，分别取其小孢子处于单核早期的幼穗，单核后期、二核期、三核期的花药及旗叶进行了可溶性蛋白质的比较研究。

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用&Bio-Rad法&对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定，结果发现随时间的推移，被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势，而对于姬蜂寄生来说，在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势，但在寄生中后期，幼蜂即将完成在寄主体内的发育取食停止时，却呈大幅下降趋势，且明显低于其自身第三天的蛋白含量；对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较，姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高，但在第五天的情况大不相同，表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

The high-affinity binding of ~125I-triiodothyronirie to the nuclear extracts was demonstrated, and Ka of TRs in MG63 cell line and in rat calvaria we...

另外MG63和骨组织中TRs的MBC也存在一定差别，提示体外培养的人类成骨样细胞系中TRs数量可能减少。

Development student reading elementary text speech text of the ability be one of the target of language teaching in the high school and re- examine to cantillate to read in the text the speech the culture and education learn of function, cantillate to read is study text speech text of importance method, cantillate of classroom read preparation work to include introduction author and background, divide the line structure layer etc., but want to see demand but settle, don't make fix constant program, slice can't because it be the teaching method of old type home school simple negation it, many language master so have deep culture, language achievement bottom, might it not be read because of cantillate but receive benefit bandit shallow.

培养学生阅读浅易文言文的能力，是中学语文教学的目标之一，重新审视诵读在文言文教学中的作用，诵读是学习文言文的重要方法，课堂的诵读准备工作包括介绍作者和背景，划分结构层次等，但要视需要而定，不要搞成固定不变的程式，切不可因为它是旧式私塾的教法就简单否定它，许多语言大师所以具有深厚的文化、语言功底，莫不因诵读而得益匪浅。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。