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Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

Aerostatic bearings have been widely applied in high-speed and high-precision systems, but the need of high precision machining and low loading capacity retard their development.

中文摘要气静压轴承被广泛应用於高速与高精度系统中，但是高精度加工之需求及低承载能力也阻碍了它的发展。

The content of mineral elements in ripe fruits of M.sieversii from 78 seedings at Mohe Town,Gongliu Country were analyzed using Atomic Absorption Spectrophotometer.The results indicated that M.sieversii seedings presented considerable genetic variations in content of mineral elements with the range of variation coefficients from 24.2% to 54.0%.So it has large potential for further selection.Four elite seedlings were selected,namely large fruit type,high Ca content type,high Zn content type and high damascenone content.As the predominant component of Ily wild fruit tree forest,Malus sieversii had occupied an area of 9300ha.But the inherent reproduction system of M.sieversii and genetic diversity of the species were destructed in different degrees,as a result of farming assart,over grazing and damage by Agrilus mali.

上述结果支持&新疆野苹果可能是栽培苹果祖先种&的结论；②对巩留县莫舍乡的新疆野苹果78个实生株系果肉组织Ca、Mg、Fe、Zn、Cu、Mn6种矿质营养元素进行测定的结果表明，每种矿质元素含量单株间差异显著，变异系数在24.2％～54.0％，遗传多样性丰富，进一步选择的潜力很大；③根据新疆野苹果果实形态、香味物质和矿质元素含量等的测定结果，初步筛选出了大果型、高钙型、高锌型和大马酮型等4个特异性状单株；④调查发现，由于农田开垦、过度放牧以及苹果小吉丁虫的蔓延危害，目前伊犁野果林面积逐年减少，野苹果固有的繁育体系和遗传多样性受到不同程度的破坏。

Alcaligenes eutrophus WSH3 was selected as the strain for the production of polyβ-hydroxybutyrate and copolymer of 3-hydroxybutyrate and 3hydroxyvalerate . This study is aimed at finding an operative way leading to high PHB and PHBV productivity, high yield coefficient and high content of PHB and PHBV. The main research results are as follows

本文以真养产碱杆菌(A.eutrophus)WSH3为聚-β-羟基丁酸和3-羟基丁酸与3-羟基戊酸的共聚物的生产菌株，以实现PHB和PHBV发酵过程的高的生产强度、高的产物对基质的产率系数和高的聚合物含量为目标，进行了PHB及PHBV发酵条件的研究，主要研究内容和结果如下

The influence of these three factors on hydration heat will counteract the deficiency of high hydration heat at early-stage due to high-content and high-strength cement. In this way, the influence of temperature stress is alle viated and the durability of concrete is enhanced.

利用这三个因素的作用——减小水化热或延迟水化放热进程，可以降低因高性能混凝土水泥用量多及标号高所造成的早期高水化热的程度，缓解温差应力的影响，提高混凝土的耐久性。

Li Yong expresses, the net swims is the golden mine of a free from contamination really, but the high risk industry that it also is high investment of a need, high-tech at the same time, without ability of own research and development, will bring annihilative blow to the enterprise.

李勇表示，网游的确是一座无污染的金矿，但它同时也是一个需要高投入、高科技的高风险产业，没有自主研发能力，将给企业带来毁灭性的打击。

He explained that after 6 years of efforts, although the city-controlled regulation of the main urban areas has achieved a full coverage, the optional planning and management resolved , but at that time in order to improve the utilization of land, often the community capacity rate approvable has been too high with the floor area ratio generally between 4-6, some are even over 10, resulting in high community population density and high traffic pressure.

扈万泰解释，经6年多努力，我市虽然对主城区控规实现了全覆盖，解决了规划管理随意性大的问题，但当时为了提高土地集约利用率，往往在小区建设的容积率审批时一直过高，普通小区的容积率一般在4—6之间，有的甚至超过10，导致一个小区人口密度过高，公共活动空间少，交通压力大。

Populus X euramericana cv. 895 is one kind of fast-growing high quality trees which has just been cultivated, bearing barrenly, anti-virus, salt alkaloid, resistance moderate, short cycle to grow up, and its vegetal period is about 240 days. It has the advantages of fast-growing , high material quality , soft textile fiber , high rate of leaving the thick liquid in papermaking, and it has a broad prospect to be embarked.

895杨是刚培育出的一种速生优质杨树，耐瘠薄，抗病性强，耐中度盐碱，抗逆性强，成材周期短，年生长期达240天左右；具有速生、材质好、纤维柔软、造纸出浆率高的特点，开发前景广阔。

Multi-beam swath bathymeter is a seafloor mapping equipment which has high efficiency of measuring, high precision of measuring, high resolution of measuring. It fits for wide sweep mapping especially.

多波束条带测深仪是一种具有高测量效率、高测量精度、高分辨率的海底地形测量设备，特别适合于大面积的扫海测量作业。

Compared with the high performance dam concrete for the Three Gorges Project, the low-heat and high performance dam concrete with High Belite Cement has excellent properties as follows

高贝利特水泥的研究，是中国建材院负责的国家&九五&重点科技攻关成果，顺应了水泥胶结材的发展方向。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。