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A screening and cultivation method for Bacillus thuringiensis of high-yield high temperature resisting protease includes the following steps: high-yield high temperature resisting protease Bacillus thuringiensis Bt140 is obtained through adopting casein substrate agar to identify flat primary screen and fermenting enzyme activity measuring secondary screening; Plackett-Burman design and response surface analytical method are adopted to obtain optimal fermentation zymogenic formula and fermentation condition for Bt140 quickly; finally, shaking fermentation level reaches to 918.56U/mL.

一种高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法，采用酪蛋白底物琼脂鉴定平板初筛与发酵酶活测定复筛的方法获得高产耐高温蛋白酶苏云金芽孢杆菌Bt140；采用Plackett-Burman设计与响应面分析法快速获得了Bt140最佳的发酵产酶配方与发酵条件，最终摇瓶发酵水平可达918.56U/mL。

Combined with high sensitive detective method, such as laser-induced fluorescence, electrochemistry, chemiluminescence and mass spectrometry, microfluid chip and capillary electrophoresis is becoming one of important and useful tool in post-genome era including proteomics as the results of theirs high performance, high speed and high throughput.

在生命科学的研究中，以毛细管电泳、微流控芯片毛细管电泳为代表的高速、高效、高通量分离分析技术与以质谱、激光诱导荧光为代表的高灵敏度、高选择性检测技术的联用，已成为后基因组时代的主流技术，是最重要的研究工具之一。

In order to meet the need of economic development for large-specification roll of steel plates, technical improvements have been carried out in the hot-rolling mill. The six sets of high pressure water pump, which cannot provide high enough speed of water supply for rolling, is replaced using three sets of high pressure centrifugal pump. The working mode of three sets of high pressure centrifugal pumps is that two pumps work and another one is spare. After the project has been carried out other problems emerge, for example, the electromotor becoming very heat and the pump being frequently attainted.

为了适应经济发展对轧制大规格的热轧板卷的需要，热轧板厂对轧线进行了技改，将6台不能满足快节奏高速轧制供水的高压柱塞泵更换为3台高压离心泵，三台高压水泵采取两用一备工作状态，投运以来，又出现电机严重发热，泵频繁损坏的问题。

Three types of base asphalt and six SBS were used to produce SBS modified asphalt. The interaction of SBS modified asphalt was discussed in different states between the base asphalt and SBS based on their compositions. Through the traditional tests, such as penetration, soften point and ductility, and US SHRP tests, the results indicated that influences to affect the temperature susceptibility, high and low temperature performance, aging characteristics are base asphalt, SBS types and their dosages. The functions of penetration to viscosity and complex shear modulus G~* to complex dynamic shear viscosity η~* were set up, and then theviscosity-temperature susceptibility of wide temperature span and the calculation method of low temperature viscosity were obtained."Process" evaluation criterions, high grading critical temperature T_ and low grading criticaltemperature T_ , were suggested according to the high and low temperatureperformances of SBS modified asphalt. Using the experience of Repeated Creep and Recovery Test for Binders, a new high-temperature evaluation index,modification rutting factor G~*~(-9) was obtained. The results after RTFO and PAV aging indicated that traditional tests didnt differentiate base asphalt and SBS modified asphalt, but dynamic mechanics temperature spectrum and G*-5 black chart clearly reflected the influence of aging to the SBS modified asphalt. With the IR and GPC tests, the reason of aging to the SBS modified asphalt were due to asphalt phase oxygenated and SBS phase depredated.

论文选择3种油源的基质沥青和6种SBS改性剂制备改性沥青，通过分析基质沥青和SBS改性剂的组成结构特点，得出了不同状态下SBS改性沥青的SBS与基质沥青的相互作用机理；通过针入度、软化点、延度等常规试验以及美国SHRP试验，分析了基质沥青、SBS改性剂类型与剂量对SBS改性沥青的温度敏感性、高低温特性及老化特性的影响；建立了针入度-粘度、复数模量G~*-复数粘度η~*的换算关系，得出了宽温度域的粘温指数VTS和较低温度下粘度的计算方法；通过高低温性能分析提出了&过程&评价参数高温等级温度T_和低温等级温度T_；借鉴重复恢复与蠕变试验研究成果，得到了SBS改性沥青高温评价指标改进型抗车辙因子G~*~(-9)；RTFO和PAV老化后的性能试验结果表明，常规试验难以区分SBS改性沥青与基质沥青的差异，而动态力学温度谱、G~*-δ黑斑图可以反映老化作用对SBS改性沥青的影响，且通过IR试验和GPC试验得出SBS改性沥青老化是沥青相的氧化和SBS的降解共同引起的；通过不同温度下SBS改性沥青混合料的旋转压实SGC试验，根据粘度与剪变率的关系，提出用剪变率60(1／s)测试SBS改性沥青的粘温曲线，并按照0.17±0.02Pa.s和0.28±0.03Pa.s确定施工温度。

An ideal backlight source should provide high brightness, high efficiency, high dimming ratio and high stability.

理想的背光源应具备高均匀亮度、高效率、高调光比以及高稳定性等特性。

A semiconductor device including a high voltage element and a low voltage element, including: a semiconductor substrate having high voltage element region where the high voltage element is formed, and a low voltage element region where the low voltage element is formed; a first LOCOS isolation structure disposed in the high voltage element region; and a second LOCOS isolation structure disposed in the low voltage element region, wherein the first LOCOS isolation structure includes a LOCOS oxide film formed on a surface of the semiconductor substrate and a CVD oxide film formed on the LOCOS oxide film, and the second LOCOS isolation structure includes a LOCOS oxide film.

提供一种在确保高耐压元件的高耐压特性的同时使高耐压元件和低耐压元件具有良好特性的半导体装置。具有高耐压元件和低耐压元件的半导体装置包含：规定了形成高耐压元件的高耐压元件区和形成低耐压元件的低耐压元件区的半导体衬底；在该高耐压元件区设置的第一LOCOS隔离结构；以及在该低耐压元件区设置的第二LOCOS隔离结构。第一LOCOS隔离结构由在该半导体衬底的表面上形成的LOCOS氧化膜和其上形成的CVD氧化膜构成，第二LOCOS隔离结构由LOCOS氧化膜构成。

Why do I choose Flatland high school and Las Montanas high school in Silicon Valley and Weiyu high school in Shanghai for comparison? The three locate in the area that is equipped with more advanced and newest computers and other technologies in their countries, so their teachers and the students can have high access to ICT. Their achievements and practices with ICT can represent the coming future in ICT education of their country. This can make the research more valuable and portentous.

选取美国的弗兰德中学、兰斯蒙坦中学和我国的位育中学为案例进行比较分析，主要是因为它们都位于本国信息化程度较高的地区，有着较好的硬件设施和环境支持，其在信息技术与学校教育整合方面的探索和实践，在一定程度上代表了本国信息技术教育当前的最高水平和未来几年的发展趋势，使比较研究具有一定的现实意义和前瞻性。

Rapid drying and high quality were obtained in the bamboo shoots of thinner cut with preheating and high temperature, and high rehydrating rates were obtained in the products of thinner cut with high temperature and no preheating.

结果表明，切分厚度薄且经漂烫，干燥温度高的毛竹笋干燥速度快，制品感官质量好；切分厚度薄且未经漂烫，干燥温度高的制品复重率高。

The high-energy life and the high-level life are different concepts. High-energy lives are beings with theurgies while high-level lives are principal lives of cosmic space-times.

高能生命与高级生命是不同的概念，高能生命是具有神通的生命，高级生命是宇宙时空的主体生命。

A brief discussion on seismic design of high arch dams is made through the following three aspects:interaction of arch dams-foundation-reservoir water,dynamic stability of high arch dam abutment ,effect of transverse slots on seismic design of high arch dam,and those aspects referred to above is not enough for seismic design of high arch dam.

对高拱坝的抗震设计进行了分析，包括拱坝坝体-地基-库水动力相互作用分析、高拱坝坝肩抗震稳定分析、横缝对高拱坝抗震设计的影响，仅对高拱坝抗震设计研究的几个方面进行了概括总结。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。