查询词典 high-rise

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The Panasonic WV-NP1004 is a high quality, high efficiency, and high reliability color network camera.

在松下WV - NP1004是高质量，高效率和高可靠性的彩色网络摄像机。

Nanjing Lishui Meng wins Daomo Limited of the introduction of high-tech equipment and professional production machinery blades, mold equipment and hardware tools of medium-sized enterprises, is a professional team, the factory has more than 2,000 square meters of factory buildings , Scientific research, development, manufacture and sale in one, with the increasing market demand, our business has been a corresponding expansion of our products according to national standards and standards of production enterprises, strictly in accordance with the ISO9001 quality management system requirements , Complete the processing equipment, a reasonable manufacturing process, improve the detection means strong design capability, to provide high-quality products, the company specializing in the production of various models bending machines, Flap machine, mold and Chongjian, etc. Specifications of plastic spiral Qieli Ji hob, cutting plate machine blade, on paper at the next round Paper knife, the shape of the cardboard boxes with knives, knife type roll, spiral knives, steel rolling knife, thrown knives, plastic models with blades, Cardboard slotted knife-cutter, repair knife edge, high-alloy.

南京溧水猛胜刀模有限公司系引进高新设备与专业生产机械刀片、模具及五金工具设备的中型企业，是一个有专业人员组成的团队，工厂拥有2000多平米的厂房，集科研，开发，制造销售于一体，随着市场需求的不断增加，我们的业务也得到了相应的扩展，我们的产品均按国标及企业标准组织生产，严格按照ISO9001质量体系要求进行管理，齐全的加工设备，合理的制造工艺，完善的检测手段较强的设计能力，为客户提供高质量的产品，本公司专业生产各型号折弯机，折边机，冲剪机等模具及各种规格塑料螺旋切粒机滚刀，剪板机刀片，切纸分纸上下圆刀，各形号纸箱用刀具，横切辊刀，螺旋刀，轧钢刀，甩刀，各型号塑料用刀片，纸板开槽刀，分切刀，修边刀，高合金。

A high-performance modular exponentiation coprocessor, SEA, is presented here, and three novel ways are employed. First, a parallel binary modular exponentiation algorithm is used to decrease cycles, and a high radix Montgomery modular multiplication algorithm is modified to the radix based high radix Montgomery modular multiplication algorithm to increase the frequency; second when mapping algorithms to a systolic array, modular square and modular multiplication are alternatively computed to cover up the dependencies between iterations in the RBHRMMM algorithm and the bypass is used to eliminate the dependencies in the PBME algorithm; third, multipliers are split first, and then accumulations are compressed as partial products to decrease carry propagation delay in the critical path.

SEA是一种针对大数模幂的高性能协处理器，其主要采用如下3种加速方法：①采用二进位并行模幂算法和以基数长度为处理字长的高基数Montgomery算法；②将算法映射到脉动阵列处理结构，并交替计算平方和乘以掩盖RBHRMMM算法中的相关，同时应用定向技术消除PBME算法中的相关；③基於"先拆分乘法、后将累加压缩"的思想优化关键路径。

But after ultra-high pressure treatment, the all free amino acid can be measured except alanine and cystine and the content of asparagines is lower. The content of free amino acid is the highest in ultra-high pressure sterilize milk, the pasteurism milk is second, the ultra high temperature sterilized milk is lowest.

但是牛乳经过超高压杀菌处理以后，除丙氨酸和胱氨酸未检出，且超高压杀菌处理以后的天门冬氨酸的含量略低以外，其余游离氨基酸均可检出，而超高压杀菌牛乳中游离氨基酸的含量最高，巴氏杀菌乳中的含量次之，超高温瞬时杀菌乳中的含量最低。

The soft soil has characteristics of high water content and low anti-shears strength and high compression coefficient and low penetrability and high sensitivity.

软土的特点是含水量高，抗剪强度低，压缩系数高，渗透性差，沉降稳定历时长。

The multiyear pentad mean data are used to analyze the mid_summer 100 hPa level circulation patterns and it is found that there are two balancing modes of the South Asian High: the Tibetan High and the Iranian High.

利用多年逐候平均资料，分析得到盛夏南亚高压的两类平衡态-青藏高压和伊朗高压，它们又各可分为东西部型。

High performance concrete is high quality and high perdurable concrete .

高效混凝土是具有高质量和高耐久性的混凝土。

All facilities of machinery have been imported from Korea, at the same time that they are blessed with high precision of production capacity, according to perfective technological process, high precision of test method, scientific manage system and so on that strickly make all produce process had high quality.

总投资200万美圆，注册资本150万美圆，所有的机械加工设备都是从韩国引进并具有高精密的机械性能，以完善的加工流程，高精密的测试手段，科学的管理体系对整个生产过程加以严格的控制确保产品质量。

The qualities of the ANN, high- nonlinear ,high- permissibility of error and high- robustness, self- adaptability, online work, and so on, are adequately used in the research.

利用确定的BP网络结构，分别采用传统的BP算法、改进的带动量自适应学习率BP算法，以及L—M算法，分析、解决高层结构选型问题。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。