查询词典 high lines

Since 1996, the Rice Research Institute of Liaoning Province has laid stress on the genetic improvement of stigma exsertion rate of japonica male sterile lines. By introducing the genes of high stigma exsertion rate from indica into japonica, a group of japonica male sterile lines with high stigma exsertion rate have been successfully developed. Their stigma exsertion rate have been greatly increased from less than 30% to more than 80%, resulting in an increased outcrossing rate over 60% and a high seed production yield of 3.0~4.5 t/hm^2. Moreover, using these sterile lines, a group of new japonica hybrid rice combinations such as Liaoyou 5218 (Liao 5216A/C418), Liaoyou 1052 (Liao 105A/C52) have been developed and released to commercial production.

自1996年以来，辽宁省稻作研究所加强了粳稻不育系的柱头外露率遗传改良研究，通过导入籼稻的高柱头外露率基因，选育了一批高柱头外露率的粳型不育系，柱头外露率由原来的30%以下逐步提高到80%以上，异交结实率可达到60%以上，杂交制种产量可提高到3.0~4.5t/hm^2，并配组育成了辽优5218（辽5216A/C418）、辽优1052（辽105A/C52）等杂交粳稻新组合，在生产上得到了大面积推广应用。

The main results are shown as follows:1.Screening freezing tolerant cultivars and lines of chrysanthemumTwenty seven lines of chrysanthemum grown outdoors were screened using \'injury rate\' and \'injury index\'.There were 7 cultivars and lines,which were relatively high tolerant to freezing treatment,i.e.,\'Jinbuhuan\',\'Xuetao\',\'Fuli\',\'Donglin 4\',\'Donglin 6\',\'Donglin 9\', and \'Donglin 13\'.There were 3 cultivars and lines,which were less tolerant,i.e.,\'Fenmeigui\', \'Donglin 1\',and \'Donglin 2\';The determination of electrolyte leakage,and contents of free proline,MDA,CAT,fructose,glucose and soluble sugars on 7 lines of chrysanthemums at different stages freezing showed that \'Xuetao\' and \'Donglin 4\' were more tolerant than others, and that especially \'Donglin 4\' was able to regulate its osmotic pathway to alleviate the \'injury degree\' caused by freezing conditions.2.Protein expression profile of chrysanthemum under low temperature stressTwo-dimensional liquid chromatography was applied to fractionate and analyze total protein taken from \'Donglin 4\' leaves grown at low temperature and at normal condition. Chromatography peaks of fraction were transformed to gel graph by ProteoVue software.

主要研究结果如下：1、露地菊抗冻品种和品系的筛选利用受伤率和伤害指数两项指标对27个露地菊品系进行耐低温筛选，筛选出'金不换\'、'雪涛\'、'富丽\'、'东林4号\'、'东林6号\'、'东林9号\'、'东林13号\'等7个耐低温品系，同时发现'粉玫瑰\'、'东林1号\'、'东林2号\'三个品系的耐低温能力最差；通过对不同自然低温处理时期，7个品系的电导率、丙二醛含量、脯氨酸含量、过氧化氢酶、果糖、葡萄糖和可溶性糖等指标的测定发现，'雪涛\'和'东林4号\'的耐低温能力要强于其它品系，尤其是'东林4号\'对低温的调节能力较强，在低温胁迫下，能够调节渗透途径减轻低温的伤害。2、低温胁迫下，露地菊蛋白质表达谱分析利用二维液相色谱技术，分离了'东林4号\'露地菊品系的低温处理和对照样品的总蛋白，通过ProteoVue软件分析发现：处理样品与对照相比共有蛋白差异点36个，其中显著增强表达的蛋白差异点有26个。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

A detection method for welding lines based on K-L algorithm is discussed. Infrared light source is applied to get high quality images of welding lines through CCD. Image template libraries for unwelded and welded lines are constructed and the welding line image is processed by K-L algorithm to match with the template libraries to judge if the welding line has been welded. The experiment shows that above method is effective with high precision and speed and it can be applied on real-time detection of welding lines.

提出计算机对所得图像进行滤波和图像分割，讨论了K-L算法的原理及其在红外焊缝图像检测中的应用；以红外光源照射焊缝，通过CCD摄取焊缝的图像，建立已焊和未焊的图像模板库，采用K-L算法对焊缝图像进行处理，并与模板库进行比较，得出焊缝是否焊接的结论；试验结果表明，该方法检测精度高，检测速度快，可以实时运用于对手工电弧焊的焊缝质量检测中。

Decades, the system does not always produce pipes, tiles, cement products and other traditional rod, but the development should be high (high-strength, high pressure) and large, new (new building materials and products) as a key to develop new products In detail: to develop high-strength prestressed concrete pile; apply to set up more than 110 kilovolt high voltage transmission lines and electrified railway lines, etc. Catenary large t m poles; application of new high-speed heavy rail with pre-stressed concrete sleeper; high-pressure , large-diameter prestressed concrete cylinder pipe; steel concrete and prestressed steel-concrete composite pipe; new type of reinforced concrete pipes, such as the mouth and socket bearing steel reinforced concrete pipes, square pipes, short-shaped pipes, common drains ; reinforced concrete inspection and; urban and rural construction using a variety of hollow interior wall panels and composite wall panels, concrete hollow block.

不能几十年一贯制生产管、瓦、杆等传统水泥制品，更应把发展高、大、新作为重点开发新产品，具体讲：大力发展预应力高强混凝土桩；适用于架设110 千伏以上高压输电线路和电气化铁路接触网线路等的大吨米电杆；适用高速重载铁路用的新型预应力混凝土轨枕；高压、大口径预应力钢筒混凝土管；钢板混凝土和预应力钢板混凝土复合管件；新型钢筋混凝土排水管，如钢承口和承插式钢筋混凝土排水管、方型渠、短形渠、共同渠；钢筋混凝土检查并；城乡建设用的各种空心内墙板和复合外墙板、混凝土空心砌块。

The main products of this company have professional production of various kinds of high middle and low audio and video lines (75-3,75-5) audio line, anti-theft line, anti-theft alarm line, high-rise buildings in multi-core control engineering intercom lines, computer lines, monitoring project wire series as well as the USB line, AV cable, power cord Series connector line and accessories, suitable for color TV, computer, VCD, Monitor, building intercom and reproducing apparatus supporting audio-visual equipment to use and so on.

本公司主要产品有专业生产各类高中低档音视频线（75-3，75-5）音响线、防盗线、防盗报警线、高层楼宇对讲工程多芯控制线、电脑线、监控工程线材系列以及USB线、AV线、电源线系列接插线及配件等，适合于彩电、电脑、VCD、监控器、楼宇对讲及音响视听设备等等的配套使用。

Our main products are: Digital Board with RF coaxial cables, digital bureaux symmetrical RF cables, high frequency coaxial cable, digital communications cable symmetrical, high-frequency digital transmission cables, security cables and industrial inspection, multi-structure control of an integrated multi-core cable , multimedia cables, cross-linked communications cables, control cables, shielded cables, electrical lines installed, UL electronic lines, plant rooms and so on with flame-retardant flexible cables, common backbone network cables, network cables ordinary slip, elevator cables, instrumentation cables, PTFE high temperature cables, ultra-five twisted-pair, program-controlled switchboard wiring cables, high frequency cables, low smoke zero halogen cables, various types of flame-retardant and fire-resistant cables, nylon jacket line, car line, data transmission cables, HFC access network using coaxial cable, 75 ohm foam Physics Series, CATV coaxial cable, 50 ohm series of RF coaxial cable, low loss 50 ohm series of physical foaming coaxial cable, control cable, rubber cable sets , electric welding cables, silicon rubber cables, marine cables, mining cables, PVC insulated wire and cable, computer cable, power cable, electrical wiring primer, rubber insulated wire, woven wire, high temperature wire, XLPE cables ship cables, high temperature cables, such as fire, at the same time to take a special type electric wire and cable products specifications to develop a business.

公司主要产品有：数字局用同轴射频电缆，数字局用对称射频电缆，高频同轴电缆，数字通信对称电缆、高频数字传输电缆、保安与工业检测电缆、多重结构多芯控制综合电缆、多媒体电缆，交联通信电缆，控制电缆，屏蔽电缆，电器安装线， UL电子线，机房等场合用阻燃软电缆，普通主干网络电缆，普通支路网络电缆，电梯电缆，仪器仪表电缆，聚四氟乙烯高温电缆，超五类双绞线，程控交换机配线电缆，高频电缆，低烟无卤电缆、各类阻燃和耐火电缆、尼龙护套线、汽车线、数据传输电缆，HFC接入网用同轴电缆、物理发泡75欧姆系列、CATV同轴电缆、50欧姆系列RF同轴电缆、低损耗物理发泡50欧姆系列同轴电缆，控制电缆、橡套电缆、电焊机电缆、硅橡胶电缆、船用电缆、矿用电缆、聚氯乙烯绝缘电线电缆、计算机电缆、电站用电缆、电机引接线、橡皮绝缘电线、编织电线、高温导线，交联聚乙烯电缆船用电缆、耐高温防火电缆等，同时承接特殊型号规格电线电缆产品的研制、开发等业务。

Special line: PET bottles to send the wind line, PET bottles transmission line, eight-treasure Li transportation, storage assembly line speed chain, refrigerator-line detection of, stainless steel transmission lines around with CPT, conveyor Store line, groove belt, transportation of finished Line, the size and quality control systems, improve water vat, with tailgate climbing belt, with the assembly line stands, the knife-edge transition belt turn, to roll lines, cell delivery systems, computer assembly lines, power assembly lines, power lines Raceway , To send the wind-line, high-speed sorting lines, transmission lines Raceway, drum line, after the assembly lines overhang, the chemical industry transmission lines, aging transmission lines, Tetra transmission lines, transmission lines plate chain, chain lost keel box line, spiral Chain plate line, spiral belt, a motorcycle transmission lines, motorcycle assembly line, climbing belt, a belt transmission line group, bottles of beer delivery systems, aerodynamic balance suspended, the lines between the pneumatic hoist, automotive transmission lines, light-top Transfer or devices, thermal plastic packet transmission line, stretching line wheel, food delivery lines, transmission lines, transmission lines climbing boxes, fruit cleansing transmission lines, the level of circulating conveyor chain, no power lines Raceway, no power telescopic Raceway lines, lines between the hoist, the letter-box sorting systems, conveyor suspension, tobacco conveyor, the parcel sorting conveyor systems, packet transmission lines, packet transmission line combinations, straight plate section, gravity-shelf access device , Heavy-duty power lines Raceway, heavy-duty conveyor chain, chain of beads to lose face envelope, turning plate, vehicle loading and unloading conveyor, the assembly line, assembly line, automatic transmission storage systems, feeding

特殊流水线：PET瓶风送线，PET瓶输送线，八宝粥输送骊，倍速链储装配线，冰箱检测线，不锈钢输送线，彩管绕带机，仓储输送线，槽型皮带机，成品输送线，尺寸与质量检测系统，大桶水提升设备，带挡板爬坡皮带机，带灯架装配线，刀口过渡转弯皮带机，地滚线，电池输送系统，电脑装配线，动力装配线，动力滚道线，风送线，高速分拣线，滚道线输送系统，滚筒线，后悬装配线，化工行业输送线，老化输送线，利乐包输送线，链板输送线，龙骨链输箱线，螺旋链板线，螺旋皮带机，摩托车输送线，摩托车装配线，爬坡皮带机，皮带输送线组，啤酒瓶输送系统，气动平衡吊，气动线间提升机，汽车输送线，轻型顶升移载装置，热塑膜包输送线，伸缩滚轮线，食品输送线，输送线，输箱爬坡线，水果输送清洗线，水平循环链式输送机，无动力滚道线，无动力伸缩滚道线，线间提升机，信盒分拣系统，悬挂输送机，烟草输送机，邮包输送分拣系统，邮包输送线，邮包组合输送线，直段板式机，重力式货架存取装置，重型动力滚道线，重型链式输送机，珠面链输包线，转弯板式机，装卸车输送机，装配流水线，装配线，自动仓库输送系统，上料机

From the beginning of the 1990s has so far developed more than 100 specifications of the products presses are the main bellows production lines, large-diameter PVC drainage, sewerage lines, PE large-diameter gas supply, drainage pipe production lines, plate production line, granulation production lines, Plum of the production line, PP-R pipe production lines, one-to two-pipe production lines and 240 T, 180T Profile production line, plastic extrusion machines, high-speed mixer, mixed plastics and various auxiliary units and exported to the Philippines, Vietnam, Thailand , Malaysia, Russia, Iran and other places.

从90年代初起至今共开发了100多个规格的塑机产品，主要有波纹管生产线、大口径PVC排水、排污生产线、PE大口径煤气、供、排水管材生产线、板材生产线、造粒生产线、梅花管生产线、PP-R管生产线、一模双出管材生产线及240T、180T异型材生产线、塑料挤出机、高速混合机、混合机组以及各种塑料辅机，并远销菲律宾、越南、泰国、马来西亚、俄罗斯、伊朗等地。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。