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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

The transverse wave disturbance along tangential base flow of circular vortex is discussed By using numerical method we study this instability of baroclinic flow which is mesoscale inertia wave instability on the cylindrical coordinate The influence of stratification stability N 2 , Coriolis parameter f 0 , the vertical shear of tangential wind z of the ambinent atmosphere and the latent heat on the growth rate of instability is studied From the above analysis and calculation, we can learn that there are Eady mode and mesoscale mode of transverse wave instability in circular vortex as well Furthermore we also learn that distribution character of mesoscale mode disturbance field of transverse wave instability, anomalistic "cats eye" structure of lower level and the character of the slowly spreading disturbance converges at low level and the quickly spreading disturbance converges at high level

作者中文名:李红金;陆汉城;宋晓亮;李鲲摘要:讨论涡旋大气中,存在沿切向基流传播的横波型扰动,并采用数值方法讨论了柱坐标系下圆形涡旋系统斜压气流中这类扰动的不稳定,这是一类中尺度的重力惯性波的不稳定。研究了涡旋环境大气的层结稳定度参数N2、切向风垂直切变Vz、凝结潜热、涡旋特性及科里奥利参数f0对不稳定增长率的影响。圆形涡旋中同样存在横波不稳定的Eady模态和中尺度模态,得到了中尺度模态的扰动场分布特征:流场的不规则"猫眼"结构及慢速传播的扰动均集中在低层,而快速传播的扰动均集中在高层的扰动特征。

DSP is used to realize digital controllers of velocity-loop and position-loop. Analyze and validate that dividing high-level continue controller to several low-level controllers, then discretize the low-level controllers to form series controller can depress the discretization control error.

采用DSP实现速度环和位置环的数字控制器,分析并验证了连续域高阶控制器分解成连续域低阶控制器,将低阶控制器离散后进行串联控制,这种实现方式的数字控制器与连续域控制器之间的控制误差,将远小于直接将连续域高阶控制器离散成高阶数字控制器产生的控制误差。

In contrast, DBA/2 mice from re-infected with PbANKA showed the high level of parasitemia and the same as that of primary infection; the level of IFN-γ in spleen cell super natants significantly increased on day 4 pi compared with control group (P.01); however, the level of specific IgG in serum didn't obviously elevated (P.01), and all mice died.

相比,PbANKA再感染小鼠出现高水平的原虫血症,与其初次感染水平柯近,再感染后第4d脾细胞培养上清中IFN-γ水平出现明显升高(P.01),而血清中特异性的IgG抗体水平却无明显变化,与正常小鼠水平接近,小鼠全部死亡。

Main work in low level vision include researching methods of vehicle detecting and segmentation, as well as techniques of image pretreatment, background updating and shadow removal. In middle level, techniques of vehicle tracking were treated in detail, and Parameters of traffic flow were calculated too. Methods of pedestrian recognition were discussed and alarm of peccancy in traffic was detected in high level.

低层视觉中,主要研究了车辆的检测与分割的方法,还包括图像的预处理、背景更新以及阴影消除等技术的研究;中层视觉中,主要研究车辆跟踪的方法,以及交通参数的检测;高层视觉中,对行人识别技术进行了一定的,同时研究了违规情况的报警。

Results The rate of premature labor was 9.40% in the very low-level group, 14.07% in the low-level group and 23.81% in the high-level group respectively.

结果 产妇血铅水平的高低与新生儿脐血铅的高低呈正相关(X^2=229.1, P.05)。

Hours post-hCG treatment, there was a high level of SPARC expression in newly formed corpus luteum, and this occurred until structural regression of corpus luteum when a basal level was observed in ovary matrix and moderate level in theca cells.

在结构性退化的黄体中,仅能在基质细胞观察到基本水平的SPARC表达,在发育卵泡的膜细胞有较强水平的表达。

Results:①The rate of dysacousis in infants was 9.4% in the very low-level group ,14.6% in the low-level group and 20.0% in the high-level group respectively.

铅对胎儿的听觉影响没有最低值,低水平铅暴露是导致新生婴儿听觉异常的主要原因之一。

Urban centres to the existing residential areas are hotbeds of high-level, high land prices and valuable land resources to the development of real estate development had only to the high altitude, it is the only way further thinning out the high land prices and high returns developers obtain the most direct means.

城市中心区是高层住宅得以存在的温床,居高不下的地价和宝贵的土地资源令房地产开发不得已只能向高空发展,这既是摊薄高昂地价的惟一方式,也是开发商获取高额回报的最直接手段。

In the last new years , as the demands of wireless communication system grows rapidly , the bands of microwave frequency had been saturated with various communication applications . To achieve wider spectrum for high-speed and high-capacity transmission , bands of millimeter-wave frequency are the solution of this problem . The advantage of MMW wireless communication systems is wider bandwidth comparing with microwave communication systems . To utilize the spectrum efficiently , many methods of complexly digital modulation is proposed . The more complex modulation , the higher linearity is required , and more various phase of signals are required to accomplish process of modulation . Therefore high-linearity and quadrature-phase modulator and demodulator are the emphasis of this thesis . For high level of integration , high performance and low cost , this chip is fabricated by the complementary oxide semi-conductor 0.13um process . A compact size of direct down-conversion and direct up conversion circuits will be presented in this thesis .

近年来随著无线通讯产业的蓬勃发展,微波频带已经趋近於饱和,为了追求更宽的频谱来达到更快传输速率及高资料容量的传输,宽频的毫米波频段提供了解决此问题的途径,毫米波无线通讯系统提供了相对於微波通讯系统宽频的优点,且为了更有效的使用频谱,各种不同且复杂的数位调变方式及方法被发表出来,越复杂的调变方式其所需的线性度越高,且需要更多不同的相位讯号来完成调变的过程,因此高线性度及四相位的调变及解调器将是此篇论文的研究重点,为了达到高整合性,高效能,及低成本的电路设计,我门利用互补式金氧半导体制程来完成此电路设计,因此一小面积的直接降频(Direct down-conversion)及直接升频(direct up-conversion)电路将在文中介绍。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。