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Student:Hi, This is Ryan. Is this the phone-in line?

你好，我叫Ryan。是声讯台吗？

On the back cover of their final issue was a phot ograph of an early morning country road, the kind you might find yourself hi tchhiking on if you were so adventurous.

最后一期的封底上是一张清晨乡间小路的照片，就是那种爱冒险的人等在那儿搭便车的那种小路。

The optical fiber sensor used hi oil contamination measurement is also developed. Some key techniques such as selection of light source and photoelectric cell, designs of measurement set and flow cell are analyzed.

阐述了颗粒检测原理，设计了反演求解颗粒粒度分布的函数限制算法，并讨论了光纤传感器测量系统的标定和油样清洗等问题。

You still pretending to be photojournalist?

hi，你还在伪装摄影记者吗？

Pica o The test of a real comedia i whether you laugh at him efore he ope hi mouth.

检验一个人是不是真正的喜剧家就是你是否在他未开口时就笑。

Hi, I have seen the free pattern gallery picture Stitched by Kate for Florida,...

嗨，我看到了免费模式的橱窗展示图片缝合由凯特为佛罗里达州，。。。

Tim:Hi,Jean!I have a new picture-book.

嗨，JEAN！我有一本新的图片书。

It is of great importance to find out the antigenic epitope of TRP-2 as an antigen in pigmental cells, hi order to set up a foundation to investigate the epitope of TRP-2, we studied on the cloning and expression of human TRP -2 code gene.

确定其抗原表位对于深入研究该抗原及临床应用具有极其重要的意义，为了给TRP—2抗原表位确定的研究打下基础，本研究克隆了人TRP—2编码基因并表达了TRP—2蛋白。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

Pillsbury's hi-tech expertise with frozen dough wouldn't count for much unless it had an authentic product and a secret recipe .

皮尔斯伯里在冷冻面团方面的高科技技能，如果没有一个可靠的产品和一个秘方的话

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。