查询词典 hexamer

Reerse transcription was performed with 3 g of total RNA, random hexamer (Amersham Bioscience, Buckinghamshire, United Kingdom), and Superscript II reerse transcriptase at conditions recommended by the manufacture, and then incubated with 1l of RNase at 37oC for 20 minutes.

再根据生产说明介绍的条件，从中选取3克RNA，随机六聚物（英国白金汉郡Amersham生物科学公司生产的）以及逆转录标记II进行逆转录，用1LRNA酶(Initrogen公司生产)在37度的环境中孵化20分钟。

The binding enthalpy change, the binding molar ratio and the binding equilibrium constant of harmine with hexamer are obtained.

而对d〓与潜在的抗肿瘤药物骆驼蓬碱的相互作用研究，已得到harmine与d〓结合的摩尔结合焓变，结合常数与结合摩尔比。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

It is possible that the linker proteins located in the center of APC discs may play a important role in stabilizing this loose APC hexamer.

有可能位于APC盘中心的连接蛋白对稳定这个APC疏松的六聚体起重要作用。

Through the comparison analysis for two MD simulation data, it was found that for the system with Zn2+ ions and phenol, the conformational flexibility of insulin hexamer was decreased.

用分子动力学模拟方法设计了两个模拟时间为600 ps的对比计算机模拟实验，研究了R6态的胰岛素六聚体在水溶液中的构象柔性。

CECH was found to form hexamer according to size exclusion chromatography with multi-angle light scattering detection.

多角度静态光散射实验表明该蛋白的聚合状态为三聚体遥。

The existence of a symmetrical hexamer in which all six insulin molecules have a helical conformation from B1-B20 has been observed.

结构解析结果表明，胰岛素单斜晶体中组成六聚体的六个分子的B1-B20为全螺旋构象。

In addition, an improved hexamer usage preference model, which can effectively reduce the dependence of coding potentials on C+G content by considering both the usage frequency and C+G content of hexamer, is presented.

同时，对蛋白编码区的序列统计特征也进行了研究，提出了一种改进的编码测度方法，该方法通过综合考虑六联体使用频率与六联体的C+G含量，可简便有效地减小序列编码潜力大小对序列C+G含量的依赖性。

In crystal, two APC-PY trimers associate into a hexamer by face. The assembly of two trimers within this hexamer is very similar to that of C-phycocyanin and R-phycoerythrin hexamers, but the tight level of the assembly of two trimers within this hexamer is clearly not so much as that of C-PC and R-PE hexamers.

晶体结构中，两个APC-PY的〓三聚体通过面对面的方式缔合成一个〓六聚体，该六聚体内两个三聚体的组装方式十分类似于C-藻兰和R-藻红六聚体，但六聚体内两个三聚体组装的紧密程度上显然不如C-藻兰和R-藻红六聚体。

These results reveal that the APC〓 trimers in the core cylinders of phycobilisomes favour a standard face to face manner to associate into a〓 hexamer, but probably, the APC〓 trimers cannot form a stable〓 hexamer like C-PC and R-PE hexamers due to the relatively weaker association between two trimers, but a relatively looser hexamer.

这些结果表明位于藻胆体core cylinder中的APC〓三聚体趋向于以通常的面对面的方式缔合成〓六聚体，但由于六聚体内两个三聚体之间的相对较弱的相互作用，APC三聚体很可能不能形成象C-藻兰和R-藻红一样的稳定的六聚体，而只能形成一个相对松弛的六聚体。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。