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However, the lack of a large number of and low cost laccases has become one of the most important obstacles to commercial application. And one of main solutions to this problem is heterologous expression of a laccase gene.

但是缺少大量廉价的酶源供应阻碍了漆酶商业化的应用,解决这个问题的一个主要方法就是通过漆酶的异源表达来获得大量的漆酶。

All tumors were histologically high grade (6 grade III and 10 grade IV). Three tumors showed heterologous elements, 2 osseous, and 1 rhabdomyoblastic. More often scattered than diffuse, S-100 protein staining was noted in 11 of 16 tumors and variable collagen IV staining in 10 of the 16. Immunoreactivity for p53 protein was diffuse and strong in 7 of 11 tumors. Twelve patients died within 17 months to 3 years of diagnosis, 1 was lost to follow-up, 2 are very recent cases, and 2 patients are currently alive, 1 after 2 recurrences, and another with spinal leptomeningeal metastases.

所有病例在组织学上都是高级别(6例III级和10例IV级)。3例肿瘤含有其他成分:2例含骨和1例含 rhabdomyoblastic.S-100蛋白染色显色于16例肿瘤中的11例,IV型胶原染色显色于16例中的10例,这些染色通常分散而不弥散。11例肿瘤中7例的p53蛋白免疫反应弥散且强烈。12例病人死于确诊后的17个月至3年,1例随访失败,2例是最近的病例,还有2例现在还活着,其中1例复发,另1例发生脊髓脑膜转移。

It can induce complete immune responses, provide heterologous cross protection, and can be easily prepared as multivalency vaccine. On the other hand, compared with traditional vaccines, live attenuated Salmonella typhimurium S.

而与传统疫苗相比,活减毒鼠伤寒沙门菌这一新型口服疫苗载体释放系统亦具有无需纯化抗原,无需佐剂,可避免抗原在胃内的降解和变性等优点。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主,近几年已在枯草芽胞杆菌中成功表达了多种基因,但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因,由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象,故都不是枯草芽胞杆菌的最佳选择,还有待改进。

Cerevisiae GAPp, suggesting that H. polymorpha △9-fatty acid desaturase was compatible with desaturation system of S. cerevisiae whereas the promoter of H-OLE1 gene had no activity in heterologous cells.

说明H-OLE1基因编码△9-脂肪酸去饱和酶,多形汉逊氏酵母的△9-脂肪酸去饱和酶和酿酒酵母的脂肪酸脱饱和系统相亲和,而H-OLE1基因的启动子在异源细胞中没有活性。

To investigate the cellular significance of targeting of PKCα to mitochondria by PICK1, REH cells clones stably expressing heterologous wild type and the non-interacting mutant PICK1 are selected.

为要探讨PICK1 将PKCα带到线体上对细胞功能的重要性为何,将PICK1及失去和PKCα交互作用能的PICK1 蛋白过表现於母巴球细胞REH。

However, opioid receptors are not expressed naturally in great abundance, they are relatively labile and frequently difficult to solubilize as monomers. The availability of their cDNAs makes them good candidates for overexpression in heterologous cells, which offers hope for the purification of membrane receptors for biochemical and structural studies.

在生物体内,阿片受体的含量很低,分离纯化过程中受体蛋白不稳定、易形成多聚体。cDNAs的克隆成功使阿片受体的异源高效表达成为可能,为研究阿片受体蛋白的生化特性和结构特征创造了条件。

The results demonstrate that the heterologous expression product on Xenopus oocytes is P2X_4 receptor.

第二部分pH改变对大鼠P2X_4受体激活电流的调制。

Results After HBO treatment,it was found that the passive skin allergic reaction in homozygous rats and heterologous mice all suppressed(P<0.01)compared with the control group,the number of mase cells degranulated by casein in abdominal cavity of mice was markedly decreased(P<0.01).On clinical observation,the near effects concerning symptoms and signs in 30 cases after HBO were 98.7%;follow-up 3 years,the effect was 73.7%.

结果 吸高压氧后大鼠同种被动皮肤过敏反应受抑制(P.01);小鼠耳异种被动皮肤过敏反应受抑制(P.01);酪蛋白引起脱颗粒的小鼠腹腔肥大细胞数与对照组比较明显减少(P.01)。30例患者治疗前后症状、体征比较,近期疗效99%,随访3年有效率达74%。

The prokaryote E. coli is most elaborated as a heterologous host, because most of the genes were already expressed in the strain for functional analysis.

该原核生物大肠杆菌是最阐述了作为异种主机,因为大部分的基因,已经体现在应变功能分析。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。