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Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中，我们在相同条件下，将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells，HEL)和3株传代细胞株：人肝癌细胞(Human hepatic carcinoma 3B cells，Hep-3B)；人肺腺癌细胞(Human lung carcinoma A549 cells，A549)；鼠正常成纤维细胞(Normal mouse fibroblastscells，NIH 3T3)。

Westem blot result: Bax in endochylema and bioblast of Hep-2 cell express augment in the experiment group, Bcl-2 in endochylema express diminutus, cytochrome C in endochylema express augment obviously, cytochrome C in bioblast express diminutus obviously, p20 in endochylema express augment obviously.

说明肿瘤抑素19肽诱导喉癌Hep-2细胞凋亡通过线粒体途径。

Besides, the series of synthetic compounds were tested for their capability to suppress growth of human hepatocellular carcinoma cell line Hep 3B and Hep G2, and myeloid leukemia cell line HL-60. The results showed compound 20, N-(2-amino- 3-nitrophenyl)cantharidinimide, exhibited more potent than others, but it was still less effective than cantharidin.

此外也利用斑蝥胺的衍生物对人类肝癌细胞Hep 3B和Hep G2，以及血癌细胞HL-60的细胞毒性进行测试，结果显示化合物20 [N-(2-amino-3-nitrophenyl)cantharidinimide]有较好的抑制作用，但是效果仍未比斑蝥素好。

Because the replication levels of A2 and MP4 in cell cultures or in cotton rat lungs were similar, the mixtures of A2/MP4 birus at the ratios of 1: 1, 10: 1, 100:1 and 1000:1 were in-oculated respectively in HEp-2 ceil cultures for the comparison in competitive replication.

将等量F212和母株A2混合后在HEp-2细胞中连续转代，在不同时间点采用差异噬斑法、核酸序列图谱分析法和限制性片段多态性法分析混合病毒群体中F212和A2的比例。

Results The HSV infected cell culture system we established stable for utilization: Cellular damage and abnormal growth were not found in Hep-2 cells cultured with SDS at 10%×2-3×10-1(1.25mg/ml).The Hep-2 cells mainly became swelling and falling off and few of them formed syncytium after infected by HSV. The CPE occurred time and scale of CPE depended on multiplicity of infectionof HSV.SDS inhibited HSV yield with the manner of dosage dependent.

在 HSV 感染的 Hep-2 细胞培养系统中分别观察 SDS 对 Hep-2 细胞的毒性作用、SDS 对 HSV 致 Hep-2 细胞病变的影响、SDS 对 HSV 空斑形成的阻断效应、四唑盐(diphenyl tretrazolium bromide，MTT)比色试验检测不同浓度 SDS 抗 HSV 效应、以及 SDS 和阿昔洛韦(Aciclovir， ACV)抗HSV 效应的比较。

Methods Hep G2 cells were treated with Aroclor1254,DDT and Toxaphene respectively for 24 hours,and further incubated in the medium supplemented with cytochalasin B(3?μg/ml)for 24 hours.Micronucleiwere scored in 1?000 binucleated Hep G2 cells.

方法人肝肿瘤细胞Hep G2经Aroclor1254、毒杀芬和滴滴涕染毒24 h，继续在补充细胞松弛素B(3μg/ml)的培养液中培养24 h后，计数1 000个双核细胞中的微核。

As shown,the antigen is a glycoprotein present on the surface of cells. Extracts from Hep G2 hepatocellular carcinoma cells, HT-29 colon cancer cells, SKHep-1 liver adenocarcinoma cells, BT-20 breast cancer cells as well as U937 histiocytic lymphoma cells were positive for the precipitation of Ag-Ab complex, which were later reactive with the anti-3A5 McAb staining. While extracts from normal human endothelial cells showed no precipitation in Western blot.

抗原分子量为63kDa，是一种存在于细胞膜上的糖蛋白，在肝细胞肝癌Hep G2细胞，肠癌HT-29细胞，肝管癌SK-Hep-1细胞，乳腺癌BT-20和人组织细胞淋巴瘤U937细胞膜提取物中，均有此蛋白带，并能被单抗3A5特异性地识别，而人正常内皮细胞则不见此反应带。

Results The strongest photoinactivation effect occured in the HEP-2 cells co-cultured with 1 mmol/L ALA for 8 h. The killing activity of ALA-PDT to the HEP-2 cells was positively correlated with the power density of the irradiation (r=0.88, P＜0.05).Conclusions ALA-PDT can effectively kill the cultured HEP-2 cells in vitro. The optimal killing activity was related to the time and the power density of the laser irradiation.

结果 (1)光照时机选择在ALA与HEP-2细胞培养8 h时杀伤效应最强；(2)ALA-PDT对HEP-2细胞的杀伤效应与照光能量呈正相关r=0.88，P 结论 ALA-PDT能有效地杀伤体外培养的肿瘤细胞，其杀伤最佳效果与照光时机和照光能量有关。

Dr. Sheau-Farn Yeh's lab found that taiwanin A, isolated from Taiwania Cryptomeriodes, is cytotoxic for several cancer cell lines, especially for human hepatoma Hep G2/A2 and Hep 3B/T2 cells. Besides cytotoxicity, the compound can arrest the cells at G2/M phase. Not only taiwanin A and its derivatives, but also compounds which isolated from the plane Clausena excavata and the roots of Rubia cordifolia have been found to have biological activities.

阳明大学生物化学研究所叶小帆老师实验室在筛选天然抗癌药物时，发现自台湾杉心材所分离出的一种化合物taiwanin A对於许多的肿瘤细胞株均有很强的细胞毒性，其中对人类肝癌细胞株Hep G2/A2与Hep 3B/T2，taiwanin A除了有显著的细胞毒性之外，同时也会造成细胞周期停滞在G2/M相。

Results Compared with solvent control,toxaphene increased significantly MN frequencies in Hep G2 cells at concentrations of 20,40?μmol/L respectively(P.01,P.01).No significant increase of MN frequencies were found in Hep G2 cells treated with Aroclor1254(23～184?μmol/L) and DDT17.8～60?

结果20，40μmol/L毒杀芬处理Hep G2细胞的微核率与溶剂对照相比显著增加(P.01，P.01)；经Aroclor1254(23~184μmol/L)和滴滴涕(17.8~60μmol/L)处理的Hep G2细胞的微核率与溶剂对照相比，差异无统计学意义。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。