查询词典 hemocyanin

Two haptens of mono-p-nitrophenyl phosphonates were designed and synthesized according to the structural features of the substrate and its hydrolytic transition state. The phosphonate haptens were coupled to the carrier proteins bovine serum albumin and keyhole limpet hemocyanin .

以对硝基乙酸苯酯水解反应中间体的结构信息进行分子设计，经过Arbzov等五步化学反应合成了二种对硝基苯基类膦酸酯半抗原，将其与载体蛋白（牛血清白蛋白BSA和钥孔〓血兰素KLH）偶联，制得了具不同抗原价（10～60）的半人工合成全抗原。

METHODS: Two polypeptides named peptide 1 and 2 were synthesized based on the bioinformatics analysis of the sequence of hMR-1 by using software TMHMM and DNAStar, then coupled with keyhole limpet hemocyanin for immunization.

TMHMM、DNAstar等软件分析hMR-1蛋白序列，选取2段优势抗原序列合成多肽并与钥孔戚血蓝素偶联，混合后免疫家兔。

Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth.

材料与方法选健康成年雌性SD大鼠 2 0只(购于同济医科大学实验中心动物学部)，体重 180～ 2 0 0g ，分为健康对照组(n =5)和实验组(n =15)。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIL多克隆抗体，用亲和层析法纯化抗体。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列，利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列，应用Blast软件对所选序列进行同源性分析并合成多肽，与载体蛋白钥孔戚血蓝素偶联，免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Thus we conclude that JA might be one of the potent factors, other than auxin, involving in the regulation of LR formation.3. Relationship between JA and SA on LR formation in rice seedlingMouse monoclonal antibodies against salicylic acid was produced and characterized for the first time. The McAb S2-8F, were derived from an immunogen in which 5-aminosalicylic acid (5-ASA) was conjugated to KLH (hemocyanin from keyhole limpets) through its-NH2 group.

实验结果还表明IBA对水稻幼苗侧根发生的促进作用可能并非是通过转化为吲哚乙酸(indole-3-acetic acid，IAA)而实现，因为外源IAA不能促进水稻幼苗侧根的发生，荣莉酸对水稻幼苗侧根发生的诱导效应及其与生长素和水杨酸的关系却和 IBA一样可以抑制种于根伸长及促进侧根伸长，只是IAA具有这种效应需要的浓度是IBA的20多倍。3。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了