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On day 3, 7, 15, 30 and 90, renal biopsies were performed. Renal histology was checked under light microscopy with HE and Masson's trichrome staining sections.

在肾炎不同时间点(3、7、15、30和90 d)行肾活检,HE和Masson's染色,光镜观察肾组织形态,免疫组化ABC法检测Mф、纤维连接蛋白、Ⅲ和Ⅳ型胶原。

Six rats of each group were randomly sacrificed on day 7,14,28 and 42 respectively.The histological changes of lung tissue were studied by HE and Masson's trichrome staining.Hydroxyproline level in lung tissue was measured by digestion method.Protein and mRNA expression of transforming growth factor-β1(TGF-β1) were assayed by immunohistochemistry and RT-PCR respectively.

各组分别于造模后第7、14、28及42 d随机处死6只,取肺组织行HE、Masson染色评价肺组织病理变化,消化法测定肺组织羟脯氨酸含量,分别采用RT-PCR和免疫组化法测定肺组织转化生长因子β1(TGF-β1)的mRNA和蛋白表达。

Then all the rats were sacrificed after body weighing. The liver and splenic indices were calculated. HE and Masson's trichrome staining were used to evaluate the degree of liver inflammation and fibrosis. The ultrastructure of hepatocytes was observed by transmission electron microscope.

实验结束后称量大鼠体重,处死大鼠,计算肝、脾指数;HE和Masson三色染色评价肝组织炎症和纤维化程度;透射电镜观察肝细胞超微结构的变化。

The histological sections, with HE and Masson's trichromic staining made, to evaluate the effectiveness of repair of bony defect. Results There existed new cartilage and new immature bone tissue in the bone substitute in 4 weeks after operation, as well as mass of cartilageous cells,osteoblasts, collagen tissue and more small vessels were seen.

方法高温法制备出磷酸四钙,然后在模拟体内环境下将其与无水磷酸氢钙发生水化固化反应,合成水泥型羟基磷灰石人工骨,将自制的CPC人工骨植入新西兰兔桡骨大段骨缺损模型中,术后4、8、12、16周取材,采用HE染色和Masson三色染色分析评价骨缺损修复效果。

Methods Tetra-calcium phosphate was prepared by high heating, than imitated the internal situation of humanbody, for hydrolization and solidation,to synthesize the hydroxy-calcium phosphate,as artificial bone in sement-type ,then im-planteted them into the large segmental defect of radius of New Silan rabbits model. The implanted materials were taken out at 4th, 8th, 12th, and 16th weeks after implantation. The histological sections, with HE and Masson's trichromic staining made, to evaluate the effectiveness of repair of bony defect.

方法高温法制备出磷酸四钙,然后在模拟体内环境下将其与无水磷酸氢钙发生水化固化反应,合成水泥型羟基磷灰石人工骨,将自制的CPC人工骨植入新西兰兔桡骨大段骨缺损模型中,术后4、8、12、16周取材,采用HE染色和Masson三色染色分析评价骨缺损修复效果。

Four weeks later, the vein grafts were removed ,and HE staining, P/VB staining ,Ⅷ R:Ag staining, and Oil Red "O" staining were performed. Establish hypercholesterolemic rabbit model of vein grafting with PTFE external stent on one side and unexternal stent on the other side.

建立兔的高胆固醇血症模型并在其基础上行动静脉吻合,定期测量兔的血清总胆固醇(TC,4周后行VG的HE染色、VB染色、Ⅷ因子染色、油红&O&染色。

Long filament individual with thin filament size and premature mutant were obtained in Bombyx mandarina and Antheraea yamamai respectively after they were irradiated by He-Ne laser. Thus it is important for breeding variety that has choice characters

野桑蚕、天蚕经He-Ne激光辐照后,在野桑蚕、天蚕中分别产生长茧丝细纤度个体和早熟性变异体,对优良品种选育有重要意义。

If you are interested in the above, please do not hesitate to get in touch with my colleague Yark He Tel:+86 21 2405 1404 or email: Yark.

如果您对上述活动有兴趣,请与我的同事何锴 Yark He 联系电话:+86 21 2405 1404 or 邮箱: Yark。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量,结果表明,虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin,白色闭壳肌中未检测到类胡萝卜素,其中肝胰腺除上述两种类胡萝卜素外,还含有其他多种未鉴定的类胡萝卜素;在各组织中,雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高;橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝;雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察,并未观察到异常现象;超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌,在橘红色闭壳肌中有三种肌纤维,其中疏松型肌纤维为橘红色闭壳肌特有。

After 8 weeks,PDGF-B measured with reverse transcription polymerise chain reaction and pathologic alter were observed with HE staining.

高脂喂养6周后以球囊拉伤方法建立兔颈总动脉内皮损伤模型。8周时处死动物,以逆转录聚合酶链式反应法检测动脉损伤处PDGF-B mRNA表达,HE染色观察颈动脉组织病理形态学改变。

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这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

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