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Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段，首先确定维生素B族对视网膜神经元是否有神经营养作用，并根据细胞活力找出最佳有效作用浓度；然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元，并在不同时间终止培养，收集细胞，HE染色和RGCs的免疫细胞化学染色，分别测量视网膜神经元和RGCs的轴突长度，评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用；最后，观察视网膜神经元对低糖损伤的反应，及维生素B族对受损的细胞是否有保护作用。

After active infection of HCMV, in adult NLBP patients, there were nontypical HCMV inclusion bodies in HE dyeing uropsammus smears under light microscope.

成年人HCMV活动性感染后导致的NLBP患者，尿沉渣涂片经HE染色后光镜下见不典型HCMV包涵体。

The iliac bone defect moulds were prepared and the compound scaffolds were implanted. The compound scaffolds were taken out after four and eight weeks separately, bone healing status and appearance of scaffolds were observed by eyes, the osteoplast growth status in compound scaffolds were viewed by HE and chinalizarin dyeing separately, the degradation of the compound scaffolds in vivo were observed by SEM.

制作髂骨骨缺损模型，在骨缺损处植入骨支架复合体，植入后4周、8周取材大体观察材料与周围骨愈合情况，HE染色及茜素红染色观察支架复合体内成骨细胞生长及扫描电镜观察支架体内降解情况。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

After giving perfusion 4% paraform trans-heart to internal fixation, the specimens were get and made bucco-lingual paraffin sections at lower first molar and made HE staining. The area of buccal and lingual gingival epithelial and connective tissue, the length of the longest rete pegs were measured with the image analysis system. Data were analyzed by two-way analysis of variance of factorial design.

内固定取材后制作下颌第一磨牙颊舌向石蜡切片，HE染色，利用图像分析系统分别测量颊侧和舌侧牙龈上皮面积、结缔组织面积、最长钉突长度，行完全随机分组两因素析因设计资料的方差分析。

The pathological changes of lung was observed by HE staining,and the invasive depth of peribronchial inflammatory ce...

HE染色观察肺组织的病理改变，半定量评定支气管周围炎症细胞浸润程度。

Effects of He-Ne laser irradiation on VEGF expression in periodontium of tooth movement in rabbits .

He-Ne激光对兔正畸牙周组织血管改建影响的实验研究。

Methods The HE staining and immunohistochemistry combining with computer-assisted quantitative image analysis system were adopted to examine the mean percent area densities of stroma,epithelium and glandular lumen and the index of PCNA among periurethral zone,intermediate zone and supcapsular zone of prostate specimens from 24 patients with BPH.

应用HE染色和免疫组化染色结合计算机辅助图像分析方法，对24例BPH手术标本的三个区域中组织构成和增殖细胞核抗原表达进行定量分析。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。