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Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Then they don't have to have naphthalin either," said Anna-Felicitas,"and don't all have to smell horrid in the autumn when they take their furs out.""

那么他们没有任何有萘说：&安娜费利西塔斯&，并没有都闻到了可怕的秋天时他们把自己的皮毛。&&

The wolframite, scheelite, tantalum niobite and fine spar have uneven distribution in the raw ores. Relatively, the wolframite and scheelite have coarse embedded granularity, generally 0.8 ~ 0.04 mm. The tantalum niobite and fine spar have fine embedded granularity, generally 0.2 to 0.04 mm.

原矿中黑钨矿、白钨矿、钽铌铁矿、细晶石的嵌布粒度皆为不均匀嵌布，黑钨矿与白钨矿的嵌布粒度相对较粗，一般为0.8~0.04毫米，而钽铌铁矿及细晶石的嵌布粒度则很细，一般为0.2~0.04毫米。

I have learned from the experts that f those who are 'normal' so far are only in the first stage. All they need are safe environment, basic living, and rhythm of life, so if they felt beloved, can play game, receive some presents, have a new school bag and can go to school they are fine. But, as long as the whole crisis has gone, everything back to normal live. Then they would find out things have been changed. The psychological hurt become obversely.

据专家们分析，对于大部分正常的孩子们来说，他们还处于危机后的第一阶段，需要更多的是次序和规律，被保证是安全的，满足生存的物质条件等，所以一些礼物，玩一些游戏，有新书包，有学上，有人爱就很满足了，但是当危机趋于平静的时候，他们才会发现一切都变化了，他们的心理创伤就会明显了，所以后期的工作是非常巨大的，尤其是对于失去亲人，肢体残疾的孩子，那更是挑战。

In this experiment, eight varieties, seven of which belong to species Onobrychis viciaefolia Scop, and one to O. t〓anscaucociea Grosh, have been studied. As a result, embryogenic calli have been obtained from tissue culture of the eight vatie- ties, and four of them have regenerated plantiets.

本实验对8个品种的红豆草（其中7个为Onobrychis vicia〓folia Scop，1个为O.transcaucosia Grosh）进行了组织培养，均得到了胚性愈伤组织，并得到了4个品种(O.viciaefolia)的再生植株。

The Lakers have validated their own belief that they have the best team , and they have flowed in a natural way with the basketball ever since Christmas , even without important orchestrator Jordan Farmar .

湖人证实了他们是最强球队的信念，在圣诞后他们的比赛打得更为正常了，即使小指挥官法玛尔缺席比赛。

I won't have wanted to face Inter or Man Utd now but it will be great to meet them if we have beaten Barcelona because we can outpass and outwit them and we will have the confidence then to do it.

我现在不想面对国米或曼联，不过如果我们能击败巴萨的话，接下来面对国米或曼联将会非常美妙！

In human's life all can have many ridges, actually, the strong overstride all can the calm after the storm, even if before is was being ridiculed by the human, is being forgotten by the human, is being injured by the human ......Thinks to look that, who will have happy tomorrow to have to thank...

人的一生中都会有许多的坎儿，其实，坚强的跨过去一切都会雨过天晴，即使以前是被人嘲笑著，被人遗忘著，被人伤害著……想想看，拥有美好的明天要感谢谁呢。。。

You have destroyed your vow to Eul when you met on that fateful summers noon as a young and lost map developer who didn't have a true purpose in the world and decided to ask those with more wisdom because you were afraid of being unable to live up to the great expectations bestowed upon you, down at the serene windy lake with it's glassy surface and lone fisherman in a small and easily overturnable boat hovering quite near the center worrying about feeding his family and the deers trotting off in the distance being jealously eyed by small rabbits who envied their youthfulness but at the same time were glad they were such innocent symbols of cuteness that were sometimes ignored by the American MEDIA, you have destroyed the oath you took with him as he decided to leave the glowing jeweled crown with the more exuberant youth of his dying generation.

在那个命运中的夏午，当时还是一个年轻且彷徨的地图开发员的你破坏了你和Eul的约定。因为你在世上没有一个确定的目标。所以你决定去请教那些更有智慧的家伙们，因为你无法承受如此大的期待而你为此感到恐惧，低着头看着碧波荡漾的湖水、那玻璃一样的湖面的非常靠近中心的位置有一个乘着简陋的随时都有沉船危险的轻舟的孤独的渔夫正在为养活他的家庭而感到担心。林中的小鹿们踏着碎步而在另一边小兔子们则充满嫉妒地注视着它们。兔子们羡慕它们的活力同时又为自己的可爱能作为纯真的象征而感到高兴。因为有时这一点能够迷惑美国媒体，你已经破坏了你和他在他决定把镶嵌着炽热宝石的皇冠留给更有朝气的年轻人作为他的继承人时的誓约。

In this part, the densities of sodium benzoate, sodium nitrobenzoate, sodium chlorobenzoate and sodium methylbenzoate in DMFwater mixed solvents have been measured at 298.15K with an Anton Paar Model 55 densimeter. From these densities, apparent molar volumes and partial molar volumes have been calculated, transfer volumes from water to DMF-water mixtures and substituent contributions to the transfer volumes have also been obtained. Solvent and substituent effects are discussed in detail. The Scaled Partical Theory has been applied to the ternary solutions to evaluate the partial molar volumes of cavity formation and the volumes change associated with solute-solvent interaction.

选择了含有取代基团的芳香类有机电解质，主要是苯甲酸钠、硝基苯甲酸钠、氯苯甲酸钠和甲基苯甲酸钠的邻、间、对同分异构体作为研究对象，用DMA 55型振荡管密度计测定了它们在不同质量百分数组成的DMF-水混合溶剂中的密度，利用这些密度数据，计算了表观摩尔体积和偏摩尔体积，得到了这些单取代苯甲酸钠从水到DMF-水混合溶剂中的迁移偏摩尔体积及苯环上不同位置的取代基对迁移偏摩尔体积的贡献值，讨论了溶剂效应和取代基效应，并将定标粒子理论应用于此三元电解质溶液中。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。