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DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The whole field of ethical science was thus left at the exclusive command of the writers who followed Grotius; and it still exhibits in an extraordinary degree the traces of that entanglement with Roman law which is sometimes imputed as a fault, and sometimes the highest of its recommendations, to the Grotian theory Many inquirers since Grotius''s day have modified his principles, and many, of course, since the rise of the Critical Philosophy, have quite deserted them; but even those who have departed most widely from his fundamental assumptions have inherited much of his method of statement, of his train of thought, and of his mode of illustration; and these have little meaning and no point to the person ignorant of Roman jurisprudence.

这样一来，全部伦理学的领域便完全留归追随格罗修斯的著者们控制了；它在很大程度上仍表现出和罗马法纠缠在一起的迹象，这有时被认为是对格罗修斯理论的一种过失，但有时则被认为是对它的最高贡献。

I like to see people fall in love infatuatedly, create career passionately ,to enjoy live fully ,but ,never froget the key matter .you still belong to youself ,we are all a universe,we all should have a self-satisfied spirit world ,this is safe place where have your most valuable things which nothing can destroy it .soul is a strange account note where no payment ,only have income never ,all pain and happy of life will been writen into its page as your most meaningful experience .in fact ,the pain is a income also.we as if have to self ,one self go to strive ,to pursue dream ,maybe he will success ,or he will fail ,.and another one welcome the self who strived for dream with warmly smile ,and show him the booty,even the failare still have .!

但是，不要忘记了最主要的事情：你仍然属于你自己。每个人都是一个宇宙，每个人都应该有一个自足的精神世界。这是一个安全的场所，其中珍藏着你最珍贵的宝物，任何灾祸都不能侵犯它。心灵是一本奇特的账簿，只有收入，没有支出，人生的一切痛苦和欢乐，都化作宝贵的体验记入它的收入栏中。是的，连痛苦也是一种收入。人仿佛有了两个自我，一个自我到世界上去奋斗，去追求，也许凯旋，也许败归，另一个自我便含着宁静的微笑，把这遍体汗水和血迹的哭着笑着的自我迎回家来，把丰厚的战利品指给他看，连败归者也有一份。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果：通过对数据的处理和分析，我们初步发现：1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域，小脑左侧更明显，左侧颞叶皮层激活点多于右侧，且脑部左侧深层激活点多于右侧；2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域，激活点主要集中表现在大脑皮层，以颞叶为主，小脑及深部未发现明显激活点；3、非穴真针刺组结果分析初步表明，BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点，大脑左、右侧均有激活点，但右侧更多，双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点，激活点主要集中在大脑皮层，深部激活点主要在壳核；4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点，主要分布在大脑的右侧，左侧也有不少激活点，就其具体分布主要在颞叶和额叶，少部分分布在顶叶和枕叶；5、外关穴真针刺与假针刺对比发现，BA8区和小脑等区域有明显的激活点；6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点；7、外关穴假针刺与非穴假针刺对比发现，BA7、8、9、18等区有激活点，主要反应点在左侧大脑的枕叶中回、楔叶，顶叶楔前叶及额上回、中回，就其分布不难看出主要在大脑中部，颞叶未见明显激活点。

This text analyzes anna from the female doctrine angle, this is the textual breakthrough to order to order with innovation.the thesis sees annas love, marriage and her behavior from the female doctrine angle first, anna has power to pursue personal happiness, anna is requesting happy, pursue the conflict of oneself liberation medium, she once resists and fight shot to lead, but unfortunate of the marriage trampled upon her ocean to overflow angry mind and body, unfortunate of love again make the hope that she lives fall through;then the reason analyzed annas tragedy from the female doctrine angle, the morals of the traditional patriciate standard and religion realize a check and supervision annas life, her life is very narrow, anna didnt chase oneself oneself of the liberation together social liberation contacts to have no category that break propertied class characters liberation mutually, end still drive crime of ages demolish;put forward annas realistic meaning finally, the social women relieve nowadays of road still very difficult, conduct and actions is a female, have to have anna that kind of to pursue personal happy courage but should also self-renewal independently, have to have higher life ideal, dont regard as the only happy exit of affections.

本文从女性主义角度分析安娜，这是本文的切入点。论文首先分析了安娜的独立意识的觉醒，安娜有权追求个人幸福，安娜在要求幸福，追求自身解放的斗争中，她反抗过、搏击过，但不幸的婚姻摧残了她的生命力，不幸的爱情又使得她生活的希望破灭；然后分析了女性出路问题，传统的贵族社会的道德标准和宗教意识制约着安娜的生活，她的生活非常的狭隘，安娜没有把自己本身的解放同社会的解放相联系没有突破资产阶级个性解放的范畴，同时她在追求爱情的时候迷失了自我，最终还是被罪恶的时代所毁灭。女性要站立1席之地，要有安娜那种追求个人幸福的勇气但也应该独立自强，要有更高的人生理想，不要把感情当作幸福的唯1出路。

Comparing the landscape types of 1995 with that of 2005, we find out that:①the natural arbors are decreasing, with patches more fragmentation, size more odds, shapes more complex;②the area of natural shrubberies is increasing, with patches less fragmentation, shapes more simple and formulary ,the size of patches more even;③the area of natural grasses is increasing, with patches less fragmentation, shapes of patches more odds;④the area of timbers is increasing, with patches more dominance in landscape, more connectivity;⑤the area of Eucalyptus increases, with patches less fragmentation, shapes of patches more complex , more convergence, more connectivity;⑥the area of orchards is increasing, more connectivity;⑦the patches of the rubber is becoming more assemble, more connectivity, more formulary;⑧the area of plowland increases;⑨the area of reservoirs, rivers and ponds increases, with patches more assemble;⑩the area of cities decreases, with patches more dispersing, with shape more complexthe area of countries increases, with patches less connectivity, more dispersing, with shape more complex; the patches of the rest lands are more dispersing, with shape more complex;(3)In the process of economic development in the upper reaches of the Changhuajiang river watershed, a series of the ecological environment problems have appeared because a lot of landscape function broke off, and the connection of human landscape with natural landscape decreased, which aroused by the fragmentation of natural and semi-nature landscapes increased, the stability of the landscape structure decreased, as well as stress of human disturbance, resource and environment upon the landscape increased gradually.

3昌化江上游流域在经济发展进程中，自然与半自然景观破碎度增加，自然景观结构稳定性下降，人为干扰、资源和环境对景观胁迫逐渐增大，中断了许多生态过程，导致城市等人文景观与自然景观连接度很差，产生了一系列的生态环境问题。

After some 20 years of effort, more than 3,000 audio tapes have been recorded, almost 300 hand-copied and block-printed editions of the epic have been collected, and 62 volumes of the epic in Tibetan have been edited and published, with a distribution of more than three million copies. Meanwhile, over 20 volumes of its Chinese edition have been published so far, and some of them have been translated into and published in English, Japanese and French.

经过20多年的努力，现已录制了3000多盘磁带，搜集藏文手抄本、木刻本近300部，整理出版藏文版62部，发行300多万册，同时还出版了20多部汉译本，并有多部被译成英、日、法文出版。

The management that try a database, especially the school try a database has to have great capacity, the category have another of characteristics, the normal regulations management contain work have great capacity, search difficulty, not easy more recent weakness, try a database the management system can to try an execution science of management, make use of the modern computer function strong, operate the quick advantage of speed, carry on the concentration and have the preface, valid management towards trying, renew the convenience and search fast, a winding is vivid and can also pass the printer after set book be over to print directly try book, lowered the labor strength consumedly, need with the password entry system, have the certain safety.

论文价格：30元，程序价格：30元全套价格：原价60元，会员价50元摘要试卷生成的管理，特别是学校的试卷生成具有量大、种类多的特点，常规管理有工作量大、查询困难、不易更新的缺点，试卷生成管理系统可对试题实行科学的管理，利用现代电脑功能强大、运算速度快的优点，对试题进行集中、有序、有效的管理，更新方便、查询快捷、组卷灵活还可在组卷结束后通过打印机直接打印成试卷，大大降低了劳动强度，需用密码登录系统，具有一定的安全性。

The management that try a database, especially the school try a database has to have great capacity, the category have another of characteristics, the normal regulations management contain work have great capacity, search difficulty, not easy more recent weakness, try a database the management system can to try an execution science of management, make use of the modern computer function strong, operate the quick advantage of speed, carry on the concentration and have the preface, valid management towards trying, renew the convenience and search fast, a winding is vivid and can also pass the printer after set book be over to print directly try book, lowered the labor strength consumedly, need with the password entry system, have the certain safety.

该套毕业设计包括：论文+程序+开题报告+PPT，若想看更详细的资料介绍，请联系 QQ：49016366摘要试卷生成的管理，特别是学校的试卷生成具有量大、种类多的特点，常规管理有工作量大、查询困难、不易更新的缺点，试卷生成管理系统可对试题实行科学的管理，利用现代电脑功能强大、运算速度快的优点，对试题进行集中、有序、有效的管理，更新方便、查询快捷、组卷灵活还可在组卷结束后通过打印机直接打印成试卷，大大降低了劳动强度，需用密码登录系统，具有一定的安全性。

The management that try a database, especially the school try a database has to have great capacity, the category have another of characteristics, the normal regulations management contain work have great capacity, search difficulty, not easy more recent weakness, try a database the management system can to try an execution science of management, make use of the modern computer function strong, operate the quick advantage of speed, carry on the concentration and have the preface, valid management towards trying, renew the convenience and search fast, a winding is vivid and can also pass the printer after set book be over to print directly try book, lowered the labor strength consumedly, need with the password entry system, have the certain safety.

论文 字数：12970页数：48 带程序）摘要：试卷生成的管理，特别是学校的试卷生成具有量大、种类多的特点，常规管理有工作量大、查询困难、不易更新的缺点，试卷生成管理系统可对试题实行科学的管理，利用现代电脑功能强大、运算速度快的优点，对试题进行集中、有序、有效的管理，更新方便、查询快捷、组卷灵活还可在组卷结束后通过打印机直接打印成试卷，大大降低镣动强度，需用密码登录系统，具有一定的安全性。关键词：分析，设计，测试，试卷生成系统，调试，查询，打包

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。