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At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

All the study subjects had known colonic disease (a history of colorectal cancer or adenomatous polyps, ulcerative colitis, or positive radiographic findings) or were suspected to have it based on signs and symptoms such as rectal bleeding, hematochezia, melena, or positive fecal occult blood test.

所有受试者都患有结肠疾病（结直肠癌，腺样息肉，溃疡性结肠炎或影像学阳性发现），或根据症状和体征（如直肠出血，便血，黑便，或者大便潜血阳性），疑似患有上述疾病。

A Large of microseismic events have been recorded in the test.

通过信号处理与解释，确定了这些事件的空间位置。

Our magazine havs start publication for one year. After one year's test and hardening, the innovation of columns and the multivariant design have been mature. We will present readers with a totally fresh appearance.

杂志已经创刊二周年，经过二周年风雨的洗礼，无论栏目的创新和设计的多元化都已完成了成熟的蜕变，我们以崭新的面目呈现在读者面前。

Viscous oils may be diluted as necessary with a suitable sterile diluent such as isopropyl myristate shown not to have antimicrobial activity in the conditions of the test.

如需要，粘稠油质可以用适合的无菌稀释剂进行稀释，例如已证实在该试验条件下不具有抗微生物活性的豆蔻酸异丙酯。

Systematic infection of mice is used as a model to test the virulence of several mutants. According with their ability to form hyphae, nonfilamentous mutant is avirulent, and hyphae defective mutants have decreased virulence.

用系统感染小鼠模型分析这些突变株的致病能力，同它们形成菌丝的能力相一致，野生菌的致病能力最强，形成菌丝弱的突变株致病能力也减弱，不能形成菌丝的cph1 efg1突变株和crk1则无毒性。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Test holes have confirmed that the site was occupied as early as the Bronze Age, with a sequence of pottery finds that extend forward to Middle Geometric times, when the clay-floored peripteral temple was constructed, in the second half of the eighth century BCE.

巴比伦空中花园当然不是吊於空中，这个名字纯粹是是出自对希腊文paradeisos 一字的意译。纯粹是因为人们把原本除有「吊」之外，还有「突出」之意的希腊文「kremastos」及拉丁文「pensilis」错误翻译所致。

I have chosen my approach as a 'Sinological experiment,' because I believe that Sinology is not exhausted by use of Western methodology, and that one part of Sinology should be to test our own ability, as Sinologists, to apply Chinese methodologies.

我选择了一种类似于&汉学实验&的读解方法，因为我觉得，汉学不应局限于使用西方的方法论，而应该充分运用中国的方法论，这也可以帮助我们这些汉学家检验自己的研究能力。

Fhe second part elaborates the procedure and the method of this cxpcriment.According to the achievement analysis of the final English examination (July, 1999) for Junior One in Wuzhou CLty, we applied the English Learning Strategies Inquiry Paper(Oxfoxd, 1993) to alt the samples. After being made t-Test~ to make sure that they have no obvious dissimi Ian tics in English achievement and strategies level,3 experiment classes and 3 contrast classes are chosen and dovided to 3 groups. Then they are trained half a year in 3 different ways:Group instructing Method, Self-learning---3--and-discussing Method and Permeating-in-teaching Method. The thirt part------Results and Analysis.

研究结果表明，不同的策略训练方祛、不同性别、不同成绩以及不同的训练时间对训练的效果均有不同程度的影响。1、分组指导法、自学讨论法和随堂渗透法这三种训练方式取得了不同的训练效果。2、从整体上说，男、女生英语学习策略的短期和长期训练效果都非常显著，但自学讨论法学生的短期学习策略训练效果存在性别差异。3、在不同成绩组学生的英语学习策略训练中，其短期训练效果有显著的差异，但其长期训练的效果则基本一致。4、英语学习策略训练的时问长短不同，训练效果的大小就不同。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。