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It is, therefore,of great importance to have a better understanding of the general framework of Japan's collegiate enrollment system for China, a country in which achievement test plays a dominant role in her collegiate entrance exam, to find references to this subject.
日本是吸取了我国传统文化思想的国家之一,了解并认清日本高校招生制度的基本结构,对像日本一样成绩考试占高校招生主导地位的中国来说,具有十分重要的参考价值。
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During the postgenomics era the information about genomic sequence and gene functions provides a new foundation for evolutionary biology and ecology As the first whole-genome sequenced plant Arabidopsis thaliana and its wild relatives have played a critical role in understanding the evolution of genomics and speciation Both A halleri and A lyrata are closely related to the model species A thaliana A halleri ssp gemmifera occurs in northeastern China Japan and Taiwan; while its sister A halleri ssp halleri is mainly distributed in Europe Geographical barriers such as Tienshan Mountain Range isolate these intraspecific sisters Likewise A lyrata ssp kamchatica and ssp lyrata occur in East Asia and North America respectively Such distribution patterns seem to be consistent with allopartic speciation The comparison between ancestral and extant polymorphism by multilocus can be informative about the population genetics of speciation In this study we collected and analyzed DNA sequences of 98 genes from four wild relatives of A thaliana A halleri ssp gemmifera A halleri ssp halleri A lyrata ssp kamchatica and A lyrata ssp lyrata The ancestral states of these four species were compared to each other in terms of level of genetic variation However the ancestral species at the time of speciation were substantially more polymorphic than the extant geographical populations The observations are not fully compatible with speciation by strict allopatry At some species pairs parapatric speciation seems more reasonable in speciation of Arabidopsis The 98 gene sequences are also used for the congruence test between gene genealogy and species phylogeny Only 28 genes support the species phylogeny but there are 23 genes supports another major genealogy { lyrata} thaliana Based on the phylogenetic position change of A lyrata ssp kamchatica and Ks value for each species pair suggested the recent directional gene flow between A halleri ssp gemmifera and A lyrata ssp kamchatica
阿拉伯芥是第一个完成基因体定序的开花植物,其基因体资讯提供植物学研究的重要依据;在解析阿拉伯芥属物种的亲缘关系以及种化机制等重要的演化议题时,阿拉伯芥近缘的野生物种自然成了不可或缺的关键;跟阿拉伯芥近缘的物种包括A halleri及A lyrata,其中A halleri ssp gemmifera主要分布於中国东北、日本以及台湾,与近缘的A halleri ssp halleri其分布於欧洲隔著天山及大陆的障蔽,而A lyrata ssp kamchatica主要分布於东北亚及台湾,与分布於北美五大湖的A lyrata ssp lyrata被北极圈所分隔,这样的分布模式暗示异域种化的可能。藉由多基因分析比较祖先物种与现生物种遗传歧异度的相关可提供讯息探讨种化时期的族群遗传结构,本研究针对A halleri ssp gemmifera、A halleri ssp halleri及A lyrata ssp kamchatica、A lyrata ssp lyrata四个物种,两对互为亚种的姊妹群,以阿拉伯芥为外群进行研究,在四个物种完成98个同源基因的分子序列,利用套装软体MCMCcoal来估算祖先物种的遗传变异,亦估算现生物种的核苷酸歧异度,观察到?多物种配对中祖先物种遗传多型性大於现生物种DNA歧异度,显示异域种化模型并无法完全解释阿拉伯芥属物种的种化模式,在某些物种配对间邻域种化模式应比异域种化更为可能;在基因树与物种树的比较,98个基因片段的亲缘模式只有28个是与已知物种树一致的,有23个基因其树状图支持{ lyrata} thaliana的型式,藉由kamchatica位置的变化以及估算各物种配对间的平均同义置换率,推测在A halleri ssp gemmifera与A lyrata ssp kamchatica间具有近代的单方向基因交流。
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With its unique influence, NGO's help as a government, public utilities added more channels to raise social resources of the development process in the Haidian played a good bridge and a supervisory role, on the other NGO organizations have a civic-minded at the same time representation, to assist the Government in ensuring the deal with some issues in a fair and equitable decision-making also will be more mature and concrete, to avoid the Government's major policy decisions on the imbalances and vulnerabilities.
由于其独特的影响,非政府组织的帮助下作为一个政府,公共事业增加了更多的渠道,提高社会资源的发展进程中发挥了良好的海淀桥梁和监督作用,对其他非政府组织组织有公德心的同时代表,以协助该国政府在确保处理一些问题的公正和公平的决策也将更加成熟,具体,以避免政府的重大决策上的失衡和脆弱性。
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Beichen District have a good basis for economic development and investment environment to attract a group of 500 companies at home and abroad, the introduction of a number of large compound, the construction of a number of influential good projects, I believe that these two central enterprises Michael set up a high level science and technology park project, Beichen District will promote faster economic development of the city's new energy industry cluster development, advanced manufacturing industry will play an important role in promoting.
北辰区有很好的经济发展基础和项目投资环境,吸引了一批国内外500强企业,引进了一批大院大所,建设了一批具有影响力的好项目,相信中央企业这两个高水平项目落户北辰科技园区,将促进北辰区经济更快发展,对本市新能源产业集群发展,先进制造业的发展将起到重要的推动作用。
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Fishtail wedding dress type have a very good role in the repair type, fit type of fishtail wedding dress, put on will be very significant,"thin" models will be appear at the same time charming and moving, very noble, very grand , we may as well try, there may be to imagine you do not have the effect of out Oh.
鱼尾型的婚纱礼服有很好的修型作用,合身的鱼尾型婚纱礼服,穿上会让人显的很"薄",同时款式也会让人显得妩媚动人,很高贵,很隆重,大家不妨尝试一下,也许会有你自己都想象不出来的效果哦。
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As a result of remarkable effect of new agent A, some countries frequently suffered from bird flu such as Indonesia, Vietnam and Thailand in recent years, have ordered A from Research Group through various channels, as well as many domestic large poultry farms as A has played an important role in prevention and treatment for Avian influenza.
由于A新制剂防治禽流感效果显著,印度尼西亚、越南、泰国等近年来频繁发生禽流感疫情的国家,通过不同渠道从研究课题组订购A,国内的许多大型禽类养殖场也纷纷订购,在防治禽流感方面发挥了重要的作用。
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In modern volleyball games, it is of little importance that the influence caused by players' positions, and it plays an active role in the development of volleyball games. The contribution of the Wing-spiker have a decreased trend, but the middle-blocker and the second setter have made more and more contributions to team's competitiveness year by year, and the rate of contribution which is made by the second setter is similar to that of spiker's.
不同角色位置的运动员得分差异总体上在逐渐减小,主攻在各项得分贡献上有减小趋势,而接应和副攻队员有逐渐增加的趋势。
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Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.
参考已发表的ChIL-15基因序列,设计合成引物,应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA,将其与SmalⅠ酶切处理的pUC19载体连接,构建重组质粒pUC19ChIL-15;用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因,与pMD 18-T载体相连构建重组质粒pMDChIL-15;然后用KpnⅠ/PstⅠ双酶切下mChIL-15基因片段,定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中,构建重组质粒pP_RoChIL-15,对其测序确认读框正确后,转入大肠杆菌DH5α中诱导表达并进行纯化,用重组ChIL-15(rChIL-15)免疫豚鼠,制备多克隆抗体;再用HindⅢ/PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因,定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中,经酶切、PCR和序列测定鉴定后,与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞,经三轮蚀斑纯化,构建重组杆状病毒rBac-ChIL-15,用该重组病毒感染处于对数生长期的Sf9细胞,按不同的时间收取样品,离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。
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Background— Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features. Mouse models of this genetic defect have been unsatisfactory because homozygote null animals die early in development and heterozygotes do not exhibit a cardiac phenotype.
背景——蛋白激酶A信号传导在心功能中发挥了重要作用早已为人们所知蛋白激酶A调节亚基因PRKAR1A的变化引起蛋白激酶A系统失调,导致了遗传性卡尼复合肿瘤综合症,其中包括的心脏粘液瘤作为它的一个主要特征此种遗传缺陷的小鼠模式仍然不能令人满意,因其纯合体空白动物在发展中早期死亡,而杂合体不能显示心脏表型
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Take marriage just as a game -- it is a game. Have a little sense of humor, that it is a role you are playing on the stage of life; but it is not something that belongs to existence or has any reality -- it is a fiction.
把婚姻仅仅当作游戏,它是一场游戏,有一种幽默感,那是你在人生的舞台上应该扮演的角色,但是那是不存在的,是不真实的,它仅仅是一个虚构的东西!
- 推荐网络例句
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The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.
采用不同浓度K2CrO4(0,0.4,0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗,测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。
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By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.
通过分析数学形态学在图像中的理论基础,研究二值图像的形态分析算法,探讨最小结构元素的两种基本形态:膨胀和腐蚀;分析了数学形态学复杂算法的基本原理,把数学形态学的部分并行处理理念引入到家实际应用中。
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Have a good policy environment, real estate, secondary and tertiary markets can develop more rapidly and improved.
有一个良好的政策环境,房地产,二级和三级市场的发展更加迅速改善。