查询词典 hapten

For developing an immunoassay for multiple residues of pyrethroid pesticides, the hapten N-2- carbanmoyl-(3"-phenoxyphenyl) methyl 3-(2"-Cldoro-3",3",3"-trifluoropropenyl)-2, 2-dimethylcyclopropane- carboxylate was synthesized from cyanohydrin and 3-(2"-Chloro-3",3",3- trifluoropropenyl)-2, 2-dimethylcyclopropanecarboxylic acid via hydrolysis and esterification. The synthesis of the hapten was foundation of preparaing anti- cyhalothrin polyclonal antibodies.

为了制备对单一拟除虫菊酯高特异性的抗体，以菊酸和氰醇为起始原料，通过水解、酯化反应合成了半抗原N-2-氨甲酰-(3-苯氧基苄基)甲基-3-(2-氯-3，3，3-三氟丙烯基)-2，2-二甲基环丙烷羧酸酯，为进一步制备抗三氟氰菊酯的特异性抗体奠定基础。

The hapten can be synthesized with a spacer attached at the aromatic moiety of the pyrethroid, through the middle of the molecule and at the cyclopropane moiety.

半抗原的合成入手点主要为菊酯分子的三碳环、中间和芳环部分，连接一个连接臂作为半抗原。

The hapten was conjugated to BSA via amino diazotization linkage.

半抗原衍生物再通过重氮化反应与BSA偶联。

The hapten synthesis of pyrethroids is introduced in this paper, as well as the development of ELISA.

主要介绍了拟除虫菊酯类农药半抗原的合成方法以及酶联免疫分析技术的研究进展和展望。

Hapten of chlorpyrifos was synthesized and the structure was identified.

本文合成毒死蜱的半抗原并对其进行了结构鉴定。

In this paper,the design of hapten synthesis,the synthesis of hapten-protein conjugation,the pre-treatment of sample as well as the application of the immunoassay in residues detection of insecticides,fungicides,herbicides,and growth regulators,were introduced and...

本文简要介绍了农药残留免疫分析方法建立中半抗原合成设计、人工免疫抗原合成、样品前处理等技术的关键环节和免疫分析技术在杀虫剂、杀菌剂、除草剂和生长调节剂等农药残留快速监测中的应用进展，并对农药残留免疫分析技术存在的问题和应用前景进行了讨论。

The hapten was conjugated to horseradish peroxidase to prepare enzyme tagged hapten by means of mixed anhydride method.

在此基础上，首次成功建立了对三唑磷具高特异性的间接竞争、包被抗体直接竞争酶联免疫吸附分析技术。

Four kinds of hapten with the similar structure of parathion-methyl were designed and synthesized. These four haptens were coupled with different length and structural spacer arms through nitro-group or methoxyphosphate group of parathion-methyl. Then six kinds of hapten-protein conjugates were synthesized by different methods.

根据甲基对硫磷的结构特征，分别从甲基对硫磷的硝基一端或磷酸酯基甲氧基一端引入一定长度的间隔臂和活性基团，设计并合成了 4种结构的半抗原；针对各种半抗原所带活性基团的性质，通过多种方法制备了与不同的载体蛋白偶联的人工抗原。

The effects of hapten structure on antibodies properties and hapten heterology on ELISA sensitivity were also investigated. The results show that haptens with simple spacer-arm can generate antibodies with desired properties, and hapten heterology can improve ELISA sensitivity significantly.

建立检测硫代磷酸二乙酯类农药的间接竞争酶联免疫分析方法，分析探讨了免疫半抗原结构对抗体特性的影响，并阐述了包被半抗原结构对ELISA灵敏度的影响规律。

According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因，是残留标示物和蛋白偶联后的特征结构发生改变，诱导产生的抗体不能够对游离MQCA很好的识别，因此，本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构，并对其合成路线进行了筛选和优化，最终以对甲基邻硝基苯胺为起始原料，经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸，为制备对游离残留标示物高亲和力的抗体，高灵敏度的ELISA残留检测方法的建立奠定基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失