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The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜,用茜素红-台盼蓝染色染色行角膜内皮细胞计数;用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变;并于正常对照组角膜比较。

The menu consists of five dishes; Half dozen chilled oysters with lemon and shallot vinegar sauce (148*), 3 pieces oyster sushi with dip (75*), 3 pieces oyster maki with sweet and sour sauce (75*), Half dozen crumbed deep fried oysters with Asian mayonnaise (148*), Half dozen oysters set on buttered spinach, glazed with white wine sauce (148*).

这款菜单包括 5道菜式,分别是:半打生蚝配柠檬和小葱醋汁(148元*),3片生蚝寿司(75元*),3片生蚝寿司卷配酸甜酱(75元*),半打炸生蚝配亚洲蛋黄酱(148元*),半打生蚝配黄油菠菜和白葡萄酒汁(148元*)。

The highest fruit set percentage was obtained when hand-pollination was done in one day (at the stage of half opened petals) before the anthers naturally dehisced. Hand-pollination could increase fruit set from 2 days to 4 hours (at the stage of tiny dehiscent petals to over half-opened petals) before the anthers naturally dehisced. The pistil compatibility was strong and fruit set percentage with hand-pollination was the highest one day before the anthers naturally dehisced (at the stage of half-opened petals).

从花药自然裂开前2 d到自然裂开前4 h(相当于花瓣呈微张开到超过半张开状态),雌蕊都具有容受性,这期间进行人工授粉能提高坐果率,但以花药自然裂开前l d(相当于花瓣呈半张开状态时),雌蕊容受性最强,人工授粉坐果率最高。

The quality of the pirouettes (half-pirouettes) is judged according to the suppleness, lightness, cadence and regularity, and to the precision and smoothness of the transitions; pirouettes (half-pirouettes) at canter also according to the balance, the elevation and the number of strides (at pirouettes 6-8, at half-pirouettes 3-4 are desirable).

3.6。定后肢回旋的品质好坏根据其柔韧性、轻盈、节奏与规律性,以及移行的精确度和流畅性来判断;以跑步做定后肢回旋时,其品质还要按其平衡性、运步高度和步数来判断(定后肢回旋为 6-8 步,定后肢半回旋为 3-4 步)。

The quality of the pirouettes (half-pirouettes) is judged according to the suppleness, lightness, cadence and regularity, and to the precision and smoothness of the transitions; pirouettes (half-pirouettes) at canter also according to the balance, the elevation and the number of strides (at pirouettes 6-8, at half-pirouettes 3-4 are desirable).

3.6。定后肢回旋的质量好坏根据其柔韧性、轻盈、节奏与规律性,以及移行的精确度和流畅性来判断;以跑步做定后肢回旋时,其质量还要按其平衡性、运步高度和步数来判断(定后肢回旋为 6-8 步,定后肢半回旋为 3-4 步)。

When both teams are executing good half-courts down the line, neither of them able to cross court and both reluctant to lift to the back, the woman who first recognizes this stalemated half-court duel and "poaches" or cuts off the next half court will usually win the rally.

当双方都在中场平抽快挡的时候,双方都很难回斜线球,并且都不愿意挑后场高球,当双方的女选手谁先意识到这样的僵局,并能够抓住机,是取得胜利的关键。

My manufacture method: Uses half bread flour to add the half mixed grain flours first (for example maize meal, broom corn millet sub-powder, sorghum powder, to be possible), mixes symmetrically, with the boiling hot boiling water synthesis pasta, and the little honey mixes with half two oils; Makes the stuffing, with the little surface, the brown sugar, the walnut not be broken, the candied and preserved fruit broken and the Dried scallop; Then the package becomes the steamed stuffed bun and light pressure Flat (best presses Discus style in order to artistic); Puts in the warm wok with cooking oil to fry in oil Sallow, a good food then completed.

我的制作方法:先用一半面粉加一半杂粮粉(如玉米粉、糜子粉、高粱粉、均可),掺和匀称,用滚烫的开水合成面团,用半两油及少许蜂蜜掺和;做馅,用少许面、红糖、核桃碎、果脯碎及瑶柱;然后包成包子并轻压成扁状(最好压成铁饼状以求美观);放入温油锅油炸到焦黄,一个美食便完成了。

Eighty SD rats were randomly divided into four groups: acute spinal cord injury groups ( SCI groups, n=20);administration of Ulinastatin (U groups, n=20), use Ulinastatin after SCI at half an- and 4- and 8- and 24- and 48- hour.;administration of Methylprednisolone (M groups, n=20), use Ulinastatin after SCI at half an- and 4- and 8- and 24- and 48- hour; administration of Ulinastatin and Methylprednisolone(M+U groups, n=20), use Ulinastatin and Methylprednisolone after SCI at half an- and 4- and 8- and 24- and 48- hour;Allen's weight drop model of acute spinal cord injury was prepared.

本课题利用Allen重物打击模型,选用SD大鼠80只,随机分为四组:脊髓损伤组(SCI组,n=20);使用乌司他丁组(乌组,n=20);使用甲基强的松龙组(甲组,n=20);乌司他丁+甲基强的松龙联合用药组(乌+甲组,n=20),所有给药组在脊髓损伤后(半小时、4小时、8小时、24小时、48小时)给药。

By applying the drop testing machine to automatically simulate half-sine pulse,the calculating model was set up to describe the course of half-sine pulse generated by the rubber waveform generator,based on the basic mechanical principle of half-sine pulse generation and non-linear of the rubber waveform generation in the condition of quasi-static compress.

采用跌落式冲击试验机机械模拟半正弦冲击波,以半正弦冲击波形产生的基本力学原理以及橡胶波形发生器在准静态压缩情况下的材料非线性为基础,建立描述橡胶波形发生器生成半正弦冲击波过程的理论计算模型。

Kutuzov simply shrugged his shoulders when they came to him one after another with projects of manuvres with the ill-shod, half-clothed, and half-starved soldiers, whose numbers had in one month dwindled to one-half without a battle, and who would even, under the most favourable circumstances, have a longer distance to traverse before they reached the frontier than they had come already.

当库图佐夫接到一个接一个的这种拙劣的作战计划时,他只耸耸肩:要执行这些计划,就要使用那些穿着破鞋、没有皮衣、饿得半死,在一个月中没有打仗就减少了一半的士兵,而且即便在最好的条件下继续追赶到边境。前面的路程比已经走过的还要远。

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She gently rebuff ed him, but agreed that they could be friends

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If in the penal farm, you were sure to be criticized.

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