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Xinjiang Daye'is higher than male sterile lines. 2、The pistil characters of the male sterile plants BC1-2、BC1-19、BC1-21、BC1-22、BC1-23 are improved. Hairy cells of stigma of the male sterile plants enwrap loose mastoid cells. The area of mastoid cells exterior is larger. Hairy cells of stigma of the male sterile plants are longer and abduction.Their Style is larger and wide. The cubage of the epidermal cell is larger and the arrangement of passage cells is tight.

BC1-2、BC1-19、BC1-21、BC1-22、BC1-23的雌蕊的性状得到改良,改良的杂交后代不育株的柱头毛状体细胞将中心的乳突细胞包裹较疏松,乳突细胞明显突起且裸露面积较大,毛状体细胞较原不育系MS-4的相对长而外展且分泌物较多,;改良的杂交后代不育株的花柱较原不育系的花柱宽且长,表皮细胞的体积较大,通道细胞排列较紧密。

The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91%, respectively.

利用野生型的发根农杆菌K599对属于不同科属的大豆、黄瓜和凤仙花进行活体感染实验,结果表明,发根农杆菌K599可使切割后的大豆、黄瓜和凤仙花子叶形成不定根,生根频率分别为100%、65%和91%。

Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒,编码NPTⅡ基因和GUS基因)转化烟草沙姆逊,获得了具有卡那霉素抗性烟草发状根,并筛选出良好的单克隆株系,该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长),通过根尖压片分析,烟草发状根不具备典型的根冠;发状根中烟碱含量1.61%稍高于与天然栽培品种母体根的含量(1.5%左右),发状根向培养液中释放烟碱,释放量最多时占总烟碱量的81.1%;在无激素的培养基上获得了再生植株。

Such as inflammation of joints, the lower leg, such as chronic venous insufficiency lead to long-term sustainability of the skin caused by congestive hairy; hyperthyroidism in Pretibial myxedema patch Department often long acuminatum out; hypothyroidism and limbs of children in the back outside a large number of hair growth; children with viral encephalitis and inter-brain barrier after mumps-induced systemic hairy; head injury, especially after the children more prone to hair; children with primary malnutrition or enteropathy, or other malabsorption, or severe infections caused by malnutrition could rise to a large number of multi-body hair; anorexia nervosa patients in the face, trunk and upper limbs have more hair grow; infant limb pain patients in the limbs, face, trunk and more hair; dermatomyositis patients, mainly in children can be located hairy forearm, leg and temporal, but also a broader scope; Berordinelli syndrome, that is, growth and maturity accelerated from an early age, and accompanied by malnutrition and intestinal muscle atrophy, common hepatomegaly and high blood fat, the skin of patients with rough, often hairy; addition of certain drugs such as streptomycin, such as cortisone can cause iatrogenic hairy; some women as a result of adrenal, ovarian and other diseases caused by increased androgen can also result in more hair.

如炎症性关节,小腿慢性静脉机能不全等导致长期持续的皮肤充血造成多毛;甲状腺机能亢进者在胫前粘液性水肿的斑块处常有粗毛长出;甲状腺机能减退的儿童背部和四肢外侧有大量毛发生长;儿童在病毒性脑炎及流行性腮腺炎后间脑障碍所致的全身性多毛;头部外伤后特别是儿童易发生多毛;儿童原发性营养不良或肠病,或其他吸收不良,或严重感染引起的营养不良均可引起大量全身性多毛;神经性厌食症患者可在面部、躯干和上肢有较多的毛发长出;婴儿肢痛病患者可在四肢、面部、躯干部多毛;皮肌炎患者,主要发生于儿童中,多毛可位于前臂、小腿和颞部,但范围也更广泛;Berordinelli综合征,即生长和成熟从小就加速,并伴有肠营养不良和肌肉萎缩,常见肝肿大和高血脂,其患者皮肤粗糙,常多毛;此外某些药物如链霉素、可的松等可造成医源性多毛;某些妇女因肾上腺、卵巢等疾病致雄性激素增多也可造成多毛。

It is to say that the patch of RAFT agent on the hairy chains.PS particles with the shell of copolymer of styrene and vinylbenzyl chloride were prepared by similar seeded and starved addition processes. RAFT agent with carboxyl end-group attached on the shell surface of PS particles by group reaction. Adding NIPA and photo-initiator into the continuous phase of polymerization system, hairy PNIPA were obtained.

最后,以表面负载一层乙烯基苄氯的亚微米级单分散聚苯乙烯微球为基质,将水溶性RAFT试剂与聚苯乙烯微球表面的官能团反应以负载到其表面,再在连续相中直接引入NIPA和光引发剂,RAFT聚合得到PNIPA毛发。

The results showed that the thermo-sensitivity of hairy PNIPA changed obviously in the presence of hairy PAA as polyelectrolyte.4-cyanopentanoic acid dithiobenzoate as soluble reversible addition-fragmentation chain transfer agent was directly added into the above PS particles with photo-initiator on the surface and NVP or NIPA as monomer.

其次,将水溶性RAFT试剂直接引入上述光引发聚合体系,探讨连续相中RAFT试剂的存在对微球表面N-乙烯基吡咯烷酮或N-异丙基丙烯酰胺聚合的影响,考察了RAFT试剂对聚合过程中微球壳层高分子毛发厚度和单体转化率变化趋势。

2D hairy brush model, 3D hairy brush model and parametric curve scheme are analyzed and various algorithms are compared.

把绘制方法分为2D,3D毛笔模型以及基于参数化曲线的纯几何图形学方法,对其中的典型算法进行了分析和比较。

Moreover,thepattern of sinigrin biosynthesized in hairy roots of B.juncea var.foliosawas the same as that in seeds,and sinigrin content in hairy roots reached29% of that in seeds.

并且叶用芥菜毛根中含有与种子中成分相同的芥子苷,毛根芥子苷含量可达种子含量的29%。

Results: The hairy root was successfully induced and the hairy root culture system was set up; The opines assay was positive for mannopine and agropine; The result of determination showed that there were a lot of anthroquinone constituents in the rhubarb hairy root and the contents of rhein, aloe-emodin, emodin or 8-O-methylchrysophanol were respectively higher than those in wild plant root.

结果:诱导出了天山大黄发根,并建立了发根离体培养系;从发根中检测到甘露碱、农杆碱的存在;定量分析发现,天山大黄发根中含有多种蒽醌类成分,其中大黄酸、芦荟大黄素、大黄素、大黄酚-8-甲醚的含量分别高于原植物根中的含量。

Methods: The medicinal plant Rheum wittrochii Lundstr., was genetically with Ri plasmid harbouring in Agrobacterium rhizogenes 9402 strain to induce hairy roots; transformed the opines from the hairy roots were assayed by paper electrophoresis; the contents of free anthroquinones such as aloe-emodin, rhein, chrysophanol, emodin, physcion, 8-O-methylchrysophanol from the hairy roots were quantitatively determined by HPLC technique.

用发根农杆菌Agrobacterium rhizogenes 9402 所含Ri质粒遗传转化药用植物天山大黄以诱导发根用纸电泳法检测发根中冠瘿碱的存在;用高效液相色谱法测定发根中芦荟大黄素、大黄酸、大黄酚、大黄素、大黄素甲醚、大黄酚-8-甲醚等游离蒽醌化合物的含量。

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