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And mices'embryos of various ages were gotten and 81 of 793 embryos had fluorescence. Secondly, we directly injected the gene/ liposome complex into 5 male mice's spermaduct and 75 of 810 embryos had fluorescence.

其中睾丸注射法、输精管注射法和外源基因/精子共孵育法分别各处理公鼠5只，7d后各配母鼠25只，结果分别冲出胚胎793枚、810枚和357枚，荧光显微镜下检测到荧光的分别有81枚胚胎(10.2%)、75枚胚胎(9.26%)和53枚胚胎(14.9%)。

Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptors mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.

以昆明系小白鼠为实验动物，取性成熟个体，先进行发情鉴定，确定其发情周期及不同的阶段，然后按发情阶段分组，分别用不同剂量的孕马血清促性腺激素和人绒毛膜促性腺激素进行超排处理并与公鼠合笼，在见栓后的第3.5天冲胚，对所得胚胎进行鉴定；将质量较好的胚胎选出，移入同期发情并经结扎公鼠交配刺激后见栓的受体小鼠子宫内，得到移植小鼠。

While he lay in the bog, the weight of sodden sphagnum moss had flattened his crushed head, and the dark waters had tanned his skin to leather and dyed his hair orange red.

在沼泽发现他时，他那业已粉碎的头部已经被厚重的水藓包裹，长时间在黑色污水中的浸泡，使他的皮肤呈现出一种类似皮革的棕色，而头发则被染成了橘红色。

The off-axis reflection system composed of four aspheric mirrors was designed, but the system structure was not so simple and the performance can also be improved, besides, manufacture and test for large aspheric mirrors are difficult; and then the spherical Fresnel mirror which is easier to be manufactured and tested was used to replace the aspheric mirror, the spherical Fresnel mirror has low cost of replication, but this design also can\'t be used in projection lens because it was only feasible in narrow wavebands; Furthermore, the design making use of catadioptric projection lens was accomplished, this design is greatly applied in rear projector because it has merits of both refraction and reflection. But it had difficulties in color aberration correction and had complex structure.

设计了四片非球面组成的离轴式反射系统，然而，仅采用非球面设计系统结构不够精简，性能也有待进一步提高，且非球面面形较大时加工和检测都存在一定的困难；继而提出了用球面Fresnel反射镜代替非球面的设计方法，虽然Fresnel反射镜加工和检测更为简单，且复制成本低，但对波长具有选择性；进而，应用折反系统结合的方式设计了离轴式投影镜头，该方法因兼具折射和反射的优点，在大屏幕超薄背投系统的设计中广为应用，缺点是会产生难以校正的色差，且结构复杂。

Then, as if he had suddenly realized where he was, he turned to the old mother who ran the place and gave her an eloquent spiel about his two friends who had come down from Paris expressly to see the joint.

后来姑娘们上床睡在他身边，用一个个吻封住他的嘴，他这才不说这些离题的话了。

To detect effects on apoptosis of cortex neuron in DHCA rats, the rat model of occluding bilateral common carotid artery in the deep hypothermic (21C) rats had been established, and flow cytometry, TUNEL, electron microscope, immunohistochemistry and RT-PCR had been studied.: part I: To establish a new rat model of cerebral ischemia/protect during DHCA: 20 SD rats were used in the experiment, little animal respirator was to assist anesthetized rat's respiration after tracheal cannula, artery press through artery spile in femoral artery, electrocardiograph, temperature, blood saturation of O2 (SaO2) and arterial blood gases were monitored during the whole experiment, blood flow of bilateral common carotid artery of rat were occluded about 60 minute when its temperature was reduced to 21 C by ice, then normal temperature was resumed.

本研究采用大鼠深低温颈总动脉阻断模型，应用流式细胞仪、电镜、原位缺口末端标记法、免疫组化法和RT-PCR技术分别检测深低温脑缺血对皮层神经细胞调亡的影响方法：第一部分深低温停循环脑保护大鼠模型的建立：SD大鼠20只，麻醉后气管插管辅助呼吸，用冰块将大鼠的体温降至(21±1)℃，阻断双侧颈总动脉，记录体温、心率、血压、血氧饱和度和血气分析，60分钟后恢复颈总动脉血流，用Longa等的5级评分标准评定4小时和24小时后大鼠神经系统损伤情况。

The child had spina bifida and had already endured a dozen operations in her short life.

孩子患有先天性脊柱裂，在她短暂的生命中已承受了十多次手术。

Flashed had collapsed. It won't eat or drink. It had become spiritless and tired.

"闪客"崩溃了，不吃不喝，日日无精打采，萎靡不振，在拍摄现场接连出错，连与摄制组工作人员的那种亲密神态也不见了。

Third, people with spirit of bushido in Japan have died as a trend along with the death of ancient power of Mufu, bushido that self-scarification and faithfulness for emperor, exactly speaking the said heroes who are willingly to sacrify their lives of flesh for spirit of loyalty and faithfulness have lost the soil that can provide basic condition of generation and growth of the bushido, especially for Japan, which had been seriously beaten by atom bomb in World War two, situation of lack of this sort of spirit can only be described as "lose something along with its root and seeds ", so the formation of Wooden Child with Iron Arms is a sort of mean of Japanese spiritsick and self-making for the bushido which had ever experienced its glories and splendidness, because spirit of self-scarification of Wooden Child with Iron Arms has already died among Japanese.

第三，日本人具有武士道精神的人随着日本古代幕府政权的消亡而渐趋衰竭，武士道为天皇孝忠的精神，明确一点讲就是为忠义精神而勇于牺牲肉身的所谓英雄已经失去了其产生和成长的土壤，特别是作为在二战遭受原子弹打击而投降的日本，这种精神的匮乏更是可以用一厥不振来形容，铁臂阿童木的形成是日本人对往昔辉煌武士道精神的一种怀念，一种意淫，因为阿童木的勇于牺牲的精神已经在日本人的精神中死亡了。

And There was no serious complication;②In the course ofinterventional therapy, Direct portal vein angiography demonstrated vena coronaria ventriculi(100%)andgastricveins(65.26%)andvenagastricaposterior38.43%, Angiography demonstrated venacoronariaventriculi communicate esophagus varicose veins, gastric veins and vena gastrica posterior communicategastric varicose veins. vena coronaria ventriculi had only a small percentage of double vein, about30.57%. The sites of vena coronaria ventriculi arising from the portal vein, splenic vein, portosplenic junction, were found in 52.06%、27.39%、20.55% respectively.③12 extrahepaticprotosystemic shunts were found in these patients. Include gastro-nephrosshuntof 3 cases, 7 caseswere splenetic- nephros shunt and 2 cases shown recanalization of umbilical vein .④The averageportal pressure before and after the procedure were 3.87±1.82kPa and 3.64±1.14kPa in 73patients, but to the time of rebleeding, it was 3.96±0.23kPa in the 11 cases.⑤There werethree kinds of variceal outcome: disappearance (54,low degree (19).⑥Spearman logisticanalyse and ANOVAtest shown liver function class, variceal degree of the splenic necrosis area,the blood direction in portal vein before operation and remain small collateral routes were thesignificant factors concerning outcome of varices.⑦The bleeding volume and portalhypertensive gastropathy are main risk factors of rebleeding.⑧The course of livercirrhosis is the risk factor of survival and extrahepatic portosystemic shunt , fine varices are thebeneficial factors to survival.⑨During all cases'followed-up data, the 1, 2, 3, 4, 5 yearcumulative survival rates and rebleeding rates were 17.81%, 28.77%, 38.36%, 43.84%, 47.95%and93.15%,91.78%,86.30%,83.56%,80.82%respectively. Conclusion The interventional disconnection treatment for liver cirrhosis and portalhypertension was designed suitability. It rapidlycontrol bleeding,butpressure of portal vein was notobvious high, perfusion was not low .it was compared with surgery therapeutic that interventionaldisconnection treatment was safe and had a significant clinical effect to hemorrhage and preventfrom rebleeding.

结果：①术后一过性发热62例(84.9%)，腹痛腹胀48例(65.8%)是介入断流术常见的并发症，未发生严重并发症；②门静脉造影显示胃冠状静脉、胃短静脉和胃后静脉的曲张分流的出现率是100%、65.26%和38.34%；显示食管静脉曲张主要由胃冠状静脉供血，胃静脉曲张主要由胃短静脉和胃后静脉供血；胃冠状静脉大多数为单支，少数为双支，其双支的出现率分别为30.57%；胃冠状静脉开口于门静脉主干的为52.06%，开口于脾静脉主干的为27.39%和开口于门脾静脉交汇处的为20.55%；③发现胃肾分流3例，脾肾分流7例、腹膜后门腔静脉分流2例，以及CTA检查发现脐静脉开放者2例；④73例患者介入断流术前和术后平均自由门静脉压力分别为3.87±1.82kpa和3.64±1.14kpa，前后比较存在显著性差异；11例再次介入手术患者的术前、术后和复发后的自由门静脉压力分别为4.02±0.24kpa、3.82±0.25kpa和3.93±0.23kpa ，前后比较发现首次术前与术后存在显著性差异，首次术前和复发出血术前门静脉压力比较无显著性差异；⑤介入术后复查曲张静脉转归基本消失54例，轻度19例；⑥Spearman相关分析和Logistic多因素回归分析，肝功能分级、静脉曲张程度、门脉血流方向和残存小侧支四个因素对曲张静脉转归有影响；Spearman相关分析和Logistic多因素回归分析门脉高压性胃病和出血量等因素对复发出血时间有影响；⑦COX回归分析，门体分流和曲张静脉转归两个因素对术后生存有影响；⑧术后随访6-70月，1、2、3、4、5年的累计复发出血率和累计生存率分别为17.81%、28.77%、38.36%、43.84%、47.95%和93.15%、91.78%、86.30%、83.56%、80.82%；结论：介入断流术治疗门脉高压食管胃底静脉曲张有独特的优点，可以快速直接控制曲张静脉出血而门静脉压力无显著增高，保证了肝脏灌注；与外科分流术相比适应证广、损伤轻、术后恢复快，不易遗漏曲张静脉；肝功能分级、曲张静脉程度、门脉血流方向和残存侧支血管对食管胃曲张静脉转归有影响；门脉高压性胃病对复发出血时间有影响；门体分流和曲张静脉转归对生存时间有影响。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。