查询词典 gustatory cells

We found, GLP-1-IR cells in various guts of rat, pig and foetus of human with distribution scatteredly among the basal portion of intestinal glands. The cell shape corresponded with open type cells, i.e. L-cells. The density of GLP-1-IR cells was different in races, but the distribution regularity of GLP-1-IR cells was same. It showed that a continuous increase from the proximal to the distal portion of small and bowel.

结果发现：在各段肠管都可见到GLP-1-IR细胞；GLP-1-IR细胞散在于肠腺的柱状上皮细胞之间，多位于肠腺的基底部，呈开放型内分泌细胞形态，即L细胞；不同种属问GLP-1-IR细胞密度不同，但分布规律相同，即自小肠和大肠的近端向远端逐渐增大。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

The results reveal that spectra obtained by different parametric methods are similar. The spectra of normal cells' voltage have only one noticeable lobe due to fluctuation of molten alumina at anodes, while spectra of other abnormal cells have one mainlobe and one sidelobe. In cells with carbon in bath the spectral mainlobe of cell voltage is still due to the fluctuation of molten alumina at anodes and the sidelobe is due to the fluctuation of carbon in bath; in cells with metal pad wave, the spectral mainlobe is due to metal pad waving and the sidelobe is still due to the fluctuation of molten alumina at anodes. Compared with wavelet packet analysis, spectral analysis is simpler and takes less time in calculation, which is a big merit in the large-scale application of online diagnosis systems of working conditions in aluminum reduction cells.

研究结果表明：不同的参数估计法得到趋于一致的频谱分析结果，正常槽只有1个尖锐谱峰，对应于阳极下熔融氧化铝涌动的特征峰，其他故障槽都有2个特征谱峰，即1个主峰和1个次峰，碳渣槽的主峰仍然对应着阳极下熔融氧化铝涌动特征峰，次峰对应悬浮碳渣的涌动特征峰；铝液波动槽主峰对应铝液波动特征峰，次峰对应着熔融氧化铝涌动的特征峰；频谱分析相对于小波包分析具有算法简单、耗时少、物理意义明确等特点，在槽况在线诊断系统的大规模推广中具有优势。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

That single cell contains the digital code to make thousands of other kinds of cells, from fat cells to bone cells -- from brain cells to lung cells.

这个单细胞包含的数字代码能做出成千上万的其他种类的细胞，即从脂肪细胞到骨骼细胞，从脑细胞到肺细胞。

METHODS: Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells. DMEM medium containing fetal bovine serum, penicillin, streptomycin and L-glutamine was added. Following several adherences and purification, the floating cells were discarded. Thus, many adherent cells with a confluence were collected. When cells were 60%-80% confluent, cells were digested by trypsin for subculture.

无菌条件下，应用Percoll密度梯度离心法分离脐血标本，收获中间层细胞，加入含胎牛血清、青霉素和链霉素、L-谷氨酰胺的DMEM基础培养液，再经反复贴壁纯化，除去悬浮生长细胞，得到较多呈融合状态的贴壁细胞，待细胞达60%~80%融合时胰酶消化传代。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

Results The morphology of endocrine cells in digestive tract was of multiplicity.The density of 5-HT cells was the highest in the colon,but not found in the esophagus,cardia gastric and cecum.The results showed that SS cells were distributed in the pylorus mostly,but not detected in the cardia,colon,cecum and rectum.Gas positive cells were the most in the duodenums,while in the esophagus and cardia was not discovered.In the cardia VIP cells were maximum,but in the esophagus,duodenums and ileum did not appear.

结果 消化道中4种免疫阳性细胞形态多样，大多呈椭圆形和锥体形。5-HT阳性细胞数量以结肠最多，空肠和回肠次之，幽门腺区、十二指肠和直肠较少，食管、贲门腺区、胃底腺区和盲肠中未见；SS阳性细胞在幽门腺区数量最多，贲门腺区、结肠、盲肠和直肠中未见；Gas阳性细胞大量出现于十二指肠，在食管、贲门腺区和盲肠未见；除个别器官未见VIP阳性细胞外，在消化道的其余各段均有分布。

The results showed that, after induced for 4~5 d by dexamethasone, beta-glycerophosphate and ascorbate-2 phosphate, the volume of some cells of the goat MSCs gradually largened and changed their form from spindle/polygon to cuboid/ovoid; With the induce time continuing, the numbers of the cuboid/ovoid cells continuous increased; At 15th day, there were many cuboid cells, and the modality of the cells changed from cuboid to ovoid; At 25th day, the oval and cuboid cells accumulation were observed.

结果表明，山羊MSCs在地塞米松、β-磷酸甘油和抗坏血酸的作用下，从诱导的第4~5天开始，即可见有些MSCs体积逐渐增大，由梭形或多角形转变为立方形或卵圆形；随着诱导时间延长，立方形或卵圆形细胞不断增多，到第15天时，有较多的立方形细胞，且这种变化经历了从立方形向卵圆形转变的过程；第25天，可见卵圆形和方形细胞堆积，诱导分化率为90.2%± 2.97%。

Methods: By the adherence , to isolate the bone marrow mesenchymal stem cells and adipose mesenchymal stem cells , to compare the characteristics of the two cells , including obtain the quantity of the cells 、 the morphos of the cells 、 the rate of adherence 、 cell kinetics 、 cell surface marker .

利用细胞贴壁性对骨髓间充质干细胞和脂肪间充质干细胞进行分离，比较两种细胞的情况，包括(1)单位质量组织获得两种细胞的量、(2)细胞形态、(3)细胞贴壁率、(4)细胞动力学、(5)细胞表面标记。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。