查询词典 gustatory cells

RESULTS: NK cells represent a unique subset of lymphocytes that have no restriction by MHC antigens and have the ability to lyses certain tumor cells without the requirement for prior immune sensitization of the host. In addition, NK cells can also produce cytokines and regulate the function of other immune cells. So, it is emerging to apply NK cells as therapeutic agents against a broad range of malignancies. In this respect, the anti-tumor activity of NK cells is the key factor of NK-cell-based immunotherapy.

结果：NK细胞是一种独特的淋巴细胞，对靶细胞的识别无MHC限制性，无需预先致敏即可直接杀伤肿瘤细胞，也可分泌细胞因子调节其他免疫细胞的功能，是机体天然免疫的主要承担者，也是获得性免疫的核心调节细胞，故NK细胞在抗肿瘤免疫中的地位越来越受到重视，随之增强NK细胞肿瘤杀伤活性的研究也逐步深入。

Some of transplanted cells expressed titin and the assembled titin could be detected in the cells adjacent to host myocardium. Cx43 could be observed between these cells. But the size and structure of transplanted cells were different from normal contractile myocardium.3. Most of the grafted cells were isolated by fibrous tissue. Part of these cells differentiated into fibrocytes. The structure of host myocardium was slightly disturbed.

MSCs植入正常心肌组织后能存活至12周，部分移植细胞表达titin，且与宿主心肌细胞相邻的细胞表达titin组装程度更高，随时间延长表达逐渐明显，其后进入平台期，但细胞体积及胞浆发育均不及正常心肌细胞；在移植细胞与宿主细胞之间可检测到Cx43的表达。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

Review articles only; calretinin; fovea centralis; macula lutea; ora serrata; photoreceptors; rods; cones; optic tract; optic nerve; visual cortex; color vision; photoreception; opsin; rhodopsin; guanine nucleotide-binding protein; G protein-coupled receptors; ion channels (cyclic GMP-gated); guanylate cyclase; cyclic GMP; dark adaptation; visual pigments; polyenes; 11-cis-retinal; vitamin A; chromophores; arrestin; recoverin; phosducin; transducin; bipolar cells; retinal ganglion cells; retinal progenitor cells; amacrine cells; Mueller cells; light; retinogenesis; ommatidia; optic vesicles; retinitis pigmentosa; blindness; macular degeneration; blind spot; Mach bands; electroretinograms; binocular vision; visual acuity; vision; retina

唯一综述；钙网膜蛋白；中央凹；黄斑；锯齿缘；光感受器；杆状细胞；圆锥细胞；视束；视神经；直观皮层；色视觉；光感受；视蛋白；视紫质；鸟苷酸-结合蛋白；G蛋白-电偶受体；离子通道；鸟苷酸环化酶；环鸟苷酸；暗适应；视色素；多烯；11 - cis -视网膜；抗干眼醇；发色团；抑制蛋白；恢复蛋白；phosducin；转导蛋白；双极细胞；视网膜神经节细胞；视网膜祖细胞；无长突细胞；米勒细胞；光；retinogenesis；小眼；视泡；色素性视网膜炎；盲的黄斑变性；盲点；马赫带；视网膜电流图；双目视觉

Calretinin; fovea centralis; macula lutea; ora serrata; photoreceptors; rods; cones; optic tract; optic nerve; visual cortex; color vision; photoreception; opsin; rhodopsin; guanine nucleotide-binding protein; G protein-coupled receptors; ion channels (cyclic GMP-gated); guanylate cyclase; cyclic GMP; dark adaptation; visual pigments; polyenes; 11-cis-retinal; vitamin A; chromophores; arrestin; recoverin; phosducin; transducin; bipolar cells; retinal ganglion cells; retinal progenitor cells; amacrine cells; Mueller cells; light; retinogenesis; ommatidia; optic vesicles; retinitis pigmentosa; blindness; macular degeneration; blind spot; Mach bands; electroretinograms; binocular vision; visual acuity; vision; retina

光感受；视蛋白；视紫质；鸟苷酸-结合蛋白；G蛋白-电偶受体；离子通道；鸟苷酸环化酶；环鸟苷酸；暗适应；视色素；多烯；11 - cis -视网膜的；抗干眼醇；发色团；抑制蛋白；恢复蛋白；phosducin；转导蛋白；双极细胞；视网膜神经节细胞；视网膜祖细胞；无长突细胞；米勒细胞；光；retinogenesis；小眼；视泡；色素性视网膜炎；盲的；黄斑变性；盲点；马赫带；视网膜电流图；双目视觉；视敏度；视觉；视网膜

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

Section in situ hybridization demonstrated that the Fstl1 transcripts in cervico-lumbar spinal cord were distributed in the gray matter neuro-cells and ependymal cells. hybridization signals were present widely in dedifferentiated cells and mesenchyme cells at 2 wks and 3 wks while also in cartilaginous cells at 3 wks of regenerating blastema after tail amputation.

原位杂交结果显示，多疣壁虎脊髓中Fstl1的阳性信号主要存在于灰质神经元细胞及室管膜细胞；而在断尾再生2~3周尾芽中，Fstl1阳性信号广泛存在于再生尾芽的新生区未分化细胞及间充质细胞，3周尾芽中，阳性信号还存在于软骨生成区。

Was presented in 12 cases (24%) by bacterial isolation. By in-situ hybridization, PCV2 signals were chiefly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and necrotic debris. PRRSV signals were mainly located at interstitial lesions and alveolar wall, and the positive signals could be found in macrophages-like cells and epithelial cells of alveoli. Swine influenza virus could be found in interstitial and necrotizing lesions, and the signals could be found in macrophages-like cells, and epithelial cells of terminal bronchiole and alveoli.

另运用原位杂交法对PCV2、PRRSV及猪流行性感冒病毒进行检测，结果发现PCV2主要分布於PNP的间质及坏死病灶区，并能於肺泡及终末细支气管上皮细胞、巨噬细胞与坏死细胞碎片皆可见有病毒核酸；PRRSV则多分布於间质病灶区及肺泡壁处，能於巨噬细胞与肺泡上皮细胞见到病毒核酸；SIV则存在於间质及坏死病灶区，且能於肺泡及终末细支气管上皮细胞与巨噬细胞见有病毒核酸。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。