查询词典 guinea-pig

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans.

几内亚的反对者责备中国和一个残暴的国家同流合污，并认为就算这桩交易成功的话，对几内亚的民众也不会有好处。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans.

几内亚的反对派则谴责中国政府不但同一个如此血腥的政权进行合作，而且拒不认同：若取消这一项目合作，是会惠及普通的几内亚民众的。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans.

几内亚的反对派谴责中国和暴力派做交易，并宣称这样的交易，即使成功几内亚民众也得不到什么好处。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans .

几内亚的反对派则谴责中方与几内亚暴戾当局媾和，并认为即使合约生效，也不可能为几内亚的平民百姓带来任何福利。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The Solomon Islands, situated in the South Pacific off the east of Papua New Guinea and northeast of Australia, is made up of more than 990 mountainous islands and low-lying coral cays.

所罗门群岛是南太平洋的一个岛国，由超过990个岛屿组成，多为火山岛和环礁岛，位於澳大利亚东北方，巴布亚新矶内亚东方。

Tip : Did you know guinea pigs eat special droppings called cecal feces?

附：你知道猪猪吃掉的自己的排泄物被称为盲肠排泄物嘛？

He had catered for silkworms, guinea-pigs, dormice, canary-birds, and dogs, without number, during his boyhood, but he had never been called upon to provide for a young person of five years old.

在他的童年时代，家里给他搜罗了蚕宝宝、豚鼠、睡鼠、金丝雀以及小狗，不计其数，但从来没有责成他为一个五岁的小孩子置备东西。

I have been in all my Circumstances a Memento to those who are touch'd with the general Plague of Mankind, whence, for ought I know, one half of their Miseries flow; I mean, that of not being satisfy'd with the Station wherein God and Nature has plac'd them; for not to look back upon my primitive Condition, and the excellent Advice of my Father, the Opposition to which, was, as I may call it, my ORIGINAL SIN; my subsequent Mistakes of the same kind had been the Means of my coming into this miserable Condition; for had that Providence, which so happily had seated me at the Brasils, as a Planter, bless'd me with confin'd Desires, and I could have been contented to have gone on gradually, I might have been by this Time; I mean, in the Time of my being in this Island, one of the most considerable Planters in the Brasils, nay, I am perswaded, that by the Improvements I had made, in that little Time I liv'd there, and the Encrease I should probably have made, if I had stay'd, I might have been worth an hundred thousand Moydors; and what Business had I to leave a settled Fortune, a well stock'd Plantation, improving and encreasing, to turn Supra-Cargo to Guinea, to fetch Negroes; when Patience and Time would have so encreas'd our Stock at Home, that we could have bought them at our own Door, from those whose Business it was to fetch them; and though it had cost us something more, yet the Difference of that Price was by no Means worth saving, at so great a Hazard.

一般人往往有一种通病，那就是不知足，老是不满于上帝和大自然对他们的安排。现在我认识到，他们的种种苦难，至少有一半是由于不知足这种毛病造成的。患有这种病的人大可以从我的一生经历中得到教训。就拿我自己来说吧，正是由于我不满自己原来的境况，又不听父亲的忠告--我认为，我有悖教训，实为我的&原罪&，再加上我后来又犯了同样的错误，才使自己落到今天这样悲惨的地步。当时，造物主已安排我在巴西做了种植园主。如果我自己不痴心妄想发财，而是满足于逐渐致富，这时候我也许已成了巴西数一数二的种植园主了，而现在我却白白地在这荒岛上流落了这么多年，过着悲惨孤寂的生活。而且，我在巴西经营时间不长；就是在这段短短的时间里，我也获利不少。因此我确信，要是我继续经营下去的话，到现在一定拥有十几万葡萄牙金币的家财了。当时，我的种植园已走上了轨道，并且日益兴旺。可是，我偏偏把这一切丢弃，甘愿去当一名船上的管货员，只是为了到几内亚去贩卖黑奴。现在想来，我为什么要这样做呢？要是我守住家业，只要有耐心，经过一段时间之后，同样可以积聚大笔财富，我不是也可以在自己的家门口，从那些黑奴贩子手里买到黑奴吗？虽说价钱贵一点，但这点差价绝不值得自己去冒这样大的风险！

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。