查询词典 guanidine

Denaturation with guanidine hydrochloride or urea is one of the primary ways of measuring the conformational stability of proteins and comparing the stability of mutant proteins.

测定天然蛋白质和蛋白质突变体构象稳定性的一个基本方法是在盐酸胍或脲作用下进行蛋白质变性研究。

Except for unstable V66W102, the guanidine and thermal denaturation of fragments are cooperative and well approximated by the two-state transition.

除了不稳定的V66W102，其它片段的盐酸胍和热变性过程协同，符合二态模型。

We dissolve it in guanidine hydrochloride, purify it with Ni++ affinity chromatography column and renature it in vitro.

同时，使用RT-PCR只涉及引物使用与保存，不涉及VSV的使用，可以避免散毒的危险。

To explore a unified mechanism of protein folding, the unfolding of pepsin induced by urea or guanidine hydrochloride has been studied by "phase diagram" method of fluorescence.

为了探索一个统－的蛋白质折迭机理，用荧光相图法分别对脲和盐酸胍诱导的胃蛋白酶的去折迭过程进行了研究。

Inclusion body was dissolved using 6M guanidine hydrochloride/2M hydrogen chloride and adding cyanogen bromide to solution Expression production was removed N terminal fusion protein AMP-A/B/C/D antibacterial activity was detected.

实验结果发现AMP-A没有抑菌活性；AMP-B/C/D均表现出广谱的抗菌活性，它们的最小抑菌浓度约为9μM，其中AMP-C对革兰氏阴性菌的抑菌效果最强，AMP-D抗菌广谱性较AMP-B/C差。

Hence this study demonstrated that hot steeping guanidine phosphate treatment is able to promote protective char layer formation and to improve red Lauan veneer flammability.

因此本研究证实磷酸胍热浸处理可促进红柳桉单板燃烧后炭层之形成，并降低单板燃烧性。

Major procurement of goods: a three-chlorosilanes, dichloromethane, AE activity ester, 3-iodine Silane, special acid, pentyl chloride, triethylamine, thiadiazole, tetrazolylazo acid, 2 - Acetamide, tetrahydrofuran, the four-guanidine, isopropanol, five phosphorus trichloride, sodium vary bitter, acid, sodium phenylacetate, 6-2 silicon n-amine, Ethylacetoacetate , Methyl isobutyl ketone, potassium dihydrogen phosphate, aluminium oxide, DL methionine, N, N-dimethylaniline, NN-diethyl aniline, 4 sodium EDTA, Anhydrous sodium sulfate, ammonium sulfate, potassium sulfate, sodium acetate, sodium carbonate, DMC, formic acid, sodium chloride medicinal, oxalate, protopine, acetone, alcohol, acetic acid, vinegar Ethyl, butyl acetate, methanol, ethanol (anhydrous, industrial, medicinal), formaldehyde, Ye Jian (30%), hydrochloride (industrial-grade, refined grade, reagent level), sulfate (98%), Ammonia, calcium carbonate, chlorine dioxide, 6 - APA ,7-ACA ,7-ADCA ,7-ANCA, sulbactam, ceftazidime activity ester, Deng salt (hydroxymethyl-K, acid precursors Potassium, sodium dihydrogen methyl), resin, the enzyme, water treatment agent, Xiao Mo agent, demulsifier, flocculants, activated carbon, all kinds of medicinal materials, All kinds of additives

三甲基一氯硅烷、二氯甲烷、AE活性酯、三甲基碘硅烷、特戊酸、特戊酰氯、三乙胺、噻二唑、四氮唑乙酸、二甲基乙酰胺、四氢呋喃、四甲基胍、异丙醇、五氯化磷、异辛酸钠、苯乙酸、苯乙酸钠、六甲基二硅胺烷、乙酰乙酸乙酯、甲基异丁酮、磷酸二氢钾、三氧化二铝、DL蛋氨酸、N，N-二甲基苯胺、NN-二乙基苯胺、乙二胺四乙酸四钠、无水硫酸钠、硫酸铵、硫酸钾、醋酸钠、碳酸钠、碳酸二甲酯、甲酸、药用氯化钠、草酸、片碱、丙酮、正丁醇、冰醋酸、醋酸乙酯、醋酸丁酯、甲醇、乙醇、甲醛、液碱（30％）、盐酸（工业级、精制级、试剂级）、硫酸（98％）、氨水、碳酸钙、二氧化氯、6－APA、7－ACA、7－ADCA 、7－ANCA、舒巴坦、头孢他啶活性酯、邓盐(羟甲基钾、前体酸钾、二氢甲基钠)、树脂、生物酶、水处理剂、消沫剂、破乳剂、絮凝剂、活性碳、各种药用辅料、各种添加剂

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

The highly sensitive chromogenic reaction of a ternary complex of 4-(2-benzothiazolylazo) pyrocatechol with Mo-, W-diphenyl guanidine and the application of partial least squares method in simultaneous spectrophotometric determination of trace Mo and W have been studied.

本文研究了新显色剂4-(2-苯并噻唑偶氮)邻苯二酚-Mo、W-二苯胍三元配合物的高灵敏显色反应以及偏最小二乘法在同时分光光度测定痕量钼和钨中的应用。

According to the prior research , a highly efficient ext raction method was modified1 High2concent ration and DNA 2f ree RNA could be attained by using CTAB/ Guanidine Thiocyanate ext racion buffer1The total RNA can reach 1.7～2.0 of the OD260/ OD280 ratio with the yield of more than 015mg per gram of f resh tissues1 The method is suitable for high2quailty total RNA ext raction in leaves , root s , corollas , seeds and developing fibers.

本研究在前人方法的基础上对快速提取棉花组织总RNA 的方法进行了探讨。利用改进的CTAB 异硫氰酸胍裂解缓冲液，获得的RNA完整性好，无 DNA 污染，含量高，每克鲜组织能提取015mg 总RNA ， OD260/ OD280比值均在117～210之间。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。