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Methods The embryo calvarial periosteum tissue was taken and, human osteoblasts were obtained by enzyme-assimilating methods. The morphological change, growth feature and osteogentic capability of osteoblasts were observel during culture in vitro, drew the growth curve was graphed and the cells was identified by alkaline phosphatase dye. At the same time, the morphology and bioactivity of 3 to 5 th-generation osbeoblasts and anabiotic cells were studied comparatively.

取人胚胎颅骨骨膜，采用酶消化法获取成骨细胞体外培养并传代，观察细胞形态，生物特性，绘制生长曲线，并经碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3~5代与冻存后成骨细胞的特点。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The first passage of YS-MSCs which grew in good condition was obtained, and adipogenic differentiation of YS-MSCs was induced by dexamethasone and insulin, and angioblast was induced by vascular endothelial growth factor and basic fibroblast growth factor.

取生长状态良好的第1代细胞，以地塞米松、胰岛素定向诱导卵黄囊间充质干细胞向脂肪细胞方向分化；以血管内皮生长因子和碱性成纤维细胞生长因子体外诱导其向血管内皮细胞方向分化。

Vascular endothelial growth factor is an angiogenic factor that promotes the growth of solid tumor by inducing angiogenesis.

实质固态的肿瘤细胞生长可藉由血管内皮生长因子激活血管的形成。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Precursors of sulfates and alkali halides prepared in co-deposition process were mixed homogenously, annealed at high temperature and then formed CoO and NiO fibers, XRD, XPS, Raman and SEM were used to characterized the samples, the growth of these fibers might be a special LS mechanism: soluble precursors in molten salt decomposed gradually to form initial crystal nucleus and succeeding particles adhered to these nucleus and grew anisotropically in the special high temperature fluent medium, the higher decomposition temperature and solubility of the precursor are two key factors in this method, which are in favor of a longer growth process and result in larger sizes of the final metal oxides crystals.

以共沉淀方法生成硫酸盐与碱金属卤化物融盐的均匀混合前驱物，在高温下退火制备出了CoO和NiO的晶须，以XRD和XPS等对成份进行了分析，SEM分析了形貌特征，研究了不同前驱物热分解特性，认为本方法是一种简单的LS生长机制：可溶性前驱物于融盐中热分解后逐渐析出成核，后续的粒子附着在核上，在融盐环境下沿一定方向作准一维生长，其产物形貌之不同源于硫酸盐的高分解温度和在融盐中的可溶性，导致晶体生长过程中成核过程与生长过程竞争的结果对一般前驱物结果的偏移。

Were studied. The optimum growth cycle of stem callus of A. crenata was 25-30d. The size of inoculums greatly influenced callus growth rate of Ardisia spp.

接种量对愈伤组织的生长影响很大，朱砂根的茎愈伤组织的接种量在1.920-3.280g／瓶之间时与生长呈负相关关系。

In the former growth period, the accumulation of various nutrient components was gradually increasing and the declining rate of crude protein content was slow; in the later growth period, the accumulation of all kinds of nutrient components easily being digested was slow until stopped. The best utilization time of Arundinaria argenteostriata was in the period from May to Sept., with the content of crude protein of 13.78%-15.10%; crude fat, 3.77%-8.49%; soluble sugar, 14.57%-18.47% and acid detergent fiber,

在其生长发育前期，各种营养物质积累速度快，粗蛋白含量下降速度缓慢；生长后期，各种容易消化的营养物质积累速度缓慢直到停止。5~9月份为铺地竹的最佳利用时期，在该生长期间其粗蛋白含量在13.78%~15.10%，粗脂肪含量在3.77%~8.49%，可利用糖含量在14.57%~18.47%，酸性洗涤纤维含量在

Solutions to these problems are put forward into Chapters 4,5, and 6 respectively, namely, a lozenged development distribution of the state-level science and technology industrial parks with Beijing, Shanghai and the Guangzhou-Shenzhen region as the primary growth poles and Wuhan and Xi抋n the secondary growth poles; a scheme of institutional innovation consisting of the introduction of a new mode of technological innovation that combines autonomous innovation with imitative innovation and the establishment of statutory regional administrative setups; and a general proposal that venture investment in China should follow the 損attern of scattered investment?.

第四章通过对中国高新区现有布局的不合理分析，提出本文以北京、上海、深穗为&主增长极&，以武汉、西安为&次增长极&的国家级高新区菱形发展布局的设想。第五章通过对中外高新区创新体系的研究，提出中国高新区自主创新与模仿创新相结合的技术创新模式和建立法定区域管理机构等制度创新方案。第六章通过对中外风险投资机理的研究，提出了中国的风险投资实行&分立模式&的总体构想。

Allelopathic effects of vanillin and cinnamic acid, two eggplant autotoxic substances, on pepper seed germination, seedling growth and mycelium growth of Fusarium oxysporum f.sp with different concentrations were studied in laboratory and bioassay experiments by a simulative way.

运用模拟的方式，采用生物测定和室内培养的方法，研究了两种茄子自毒物质香草醛和肉桂酸各浓度对辣椒种子萌发和幼苗生长的化感效应，及其对辣椒枯萎菌菌丝生长的影响。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失