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These ingredients includes epidermal growth factor, hepatocyte growth factor, dimethyl sulfoxide, phenobarbital sodium and all trans-retinoic acid. We isolate and culture primary 10-weeks-old human fetal hepatocytes, observe hepatocytes form and its function markers. Inoculate hepatocytes with HBV Dane particles to observe if HBV susceptibility of fetal hepatocytes can be induced in vitro. To detect HBV covalently closed circular DNA within hepatocytes, we improve the existent PCR method.

基于上述假设,我们设计了10种培养条件,在自行设计的无血清培养基中添加不同的细胞分化诱导物,包括表皮生长因子、肝细胞生长因子、二甲基亚砜、苯巴比妥钠、全反式维甲酸等,分离孕10周龄人胎肝细胞进行原代培养,观测肝细胞形态及功能指标变化,并定时给予HBV感染,观察能否在体外诱导胎肝细胞出现HBV易感性;为检测肝细胞内HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA),我们改进了现有的PCR检测法。

Then, the experiment that edelfosine inhibited the phosphatization of Spm1 was carried out in order to elucidate whether edelfosine affect on the phosphatization of Spm1 induced via Mid2 and inhibited via Pmp1.(1)、On the inhibition experiment of parallel growth of S. pombe wild-type cells, spm1 mutants, pmp1 mutants, mid2 mutants and relevant retransform strains, the spm1 mutants grew well treated with 5.0μM and 10.0μM edelfosine for 24 h; the growth ratio of spm1 mutants had a statistic significance in between the cells previously mentioned and the S.

(1)、通过野生型粟酒裂殖酵母和胞质分裂突变体spm1Δ、pmp1Δ、mid2Δ及其再转化株对依地福新的平行生长抵抗试验,进一步确定依地福新对spm1、pmp1、mid2基因的影响;(2)、应用依地福新对Spm1磷酸化的影响试验,阐明依地福新是否通过影响Mid2蛋白和Pmp1蛋白的表达而影响Spm1蛋白的磷酸化。

The invention has the advantages that by means of single film growth craft, supporting structures of a pier, a bridge leg, and a deck are accomplished, thus greatly lowering the difficulty and complicity of the craft; the pier, the bridge leg, and the deck are formed into a trinity, thus assuring the mechanical support of the whole micro bridge to be firmer and to have higher stability; meanwhile, the pier manufactured by means of the single film growth craft has a hollow shape, thus ensuring that the manufacturing process of the electrical channel of the micro-bridge structure is more simple, without the need of tapping in the pier; only simple photoetching craft is needed to guarantee the connection between the electrode at the upper end and the electrode at the lower end.

本发明利用一次薄膜生长工艺完成桥墩、桥腿和桥面的支撑结构,大大降低了工艺难度和复杂性,且桥墩、桥腿和桥面三位一体,使得整个微桥的机械支撑更为牢固,稳定性更高;同时,本发明利用一次薄膜生长工艺制作出的桥墩成空心状,使得微桥结构的电学通道制作过程变得更为简单,无需另外在桥墩中开孔,只需简单的光刻工艺即可保证上端电极和下端电极相连。

Effects of tillage systems and nitrogen applied amount on wheat growth and development and yield of wheat and maize 3.1 Effects of tillage systems and nitrogen applied amount on wheat growth and development In the second year of experiment, there was no significant difference in the wheat flag leaf photosynthesis rate, flag leaf senescence characteristics, photosynthate transferring amount, photosynthate transferring ratio and dry matter accumulation of between R, H and C.

3耕作模式与施氮量对小麦、玉米生长发育及产量的影响 3.1耕作模式与施氮量对小麦生长发育的影响在试验第二年,旋耕和耙耕模式在旗叶光合速率、旗叶衰老特性和光合产物转移量和转移率,以及干物质积累量上与常规耕作模式无显著差异。

Objective To observe the effects of piperazine ferulate on synthesis of extracellular matrix and expression of connective tissue growth factor in transforming growth factor-betal (TGF-β1) induced rat glomerular mesangial cells, and to investigate its mechanism on the prevention of glomerulosclerosis.

目的 探讨阿魏酸哌嗪对转化生长因子-β1(TGF-β1)诱导下肾小球系膜细胞结缔组织生长因子及细胞外基质成分表达的影响及其意义。

Gonadotropin-Ⅱ and growth hormone are the key hormones released from the anterior pituitary regulating reproductive functions and body growth, respectively.

促性腺激素与生长激素由脑下垂体前叶所释放,为调控生殖与成长最重要的激素。

But15℃is require for normal growth of plantlet growth,while high temperature for bulb induction.

因此作者认为,较低温度是大蒜绿体生长的需要,高温才是鳞茎形成的直接诱导因素。

To fully utilize the advantages of 3S technologies, crop growth simulation model and crop management knowledge model, and to grope info the integration method and application mode of key technologies in precise farming adapted to Chinese circumstance, a model and 3S-bascd digital wheat farming system was developed based on plentitude combination of wheat growth simulation model, wheat management knowledge model and 3S technologies.

为了充分发挥和利用3S技术、生长模型和管理知识模型的优势,探索适合中国国情的精准农业关键技术集成方法及应用模式,以软件开发方法论为指导,充分结合小麦生长模型、管理知识模型和3S技术,应用ASP。

In this study, 30 mgL^(-1) Zn treatment promoted the growth of root and increased zinc content and accumulation in root. If plumule was concerned, 60 mgL^(-1) Zn treatment was beneficial to their growth and zinc accumulation.

Zn30处理有利于根的生长,能显著提高根中Zn的含量并促进Zn在根中的积累;Zn60处理有利于芽的生长,能显著提高芽中的Zn含量并促进Zn在芽中的积累。

And if the current slowdown in growth momentum to continue, this year will be almost 10 years to maintain the rapid growth of household savings of the inflection point.

如果目前的经济增长放慢的势头继续下去,今年将是近10年保持快速增长,居民储蓄的拐点。

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She gently rebuff ed him, but agreed that they could be friends

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If in the penal farm, you were sure to be criticized.

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Several theories about reigniting and extinguishing of the arc have been refered.

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