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The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草毒叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本，采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系，用MTT法选择紫外线照射细胞的适宜强度；采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线；采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

The study results indicated: The growth and output of the Sabaigrass was in orderon condition that soil holding water ratio was between 70% and 80%. The segregatingbranch of Sabaigrass have not grow rapidly in the procreative period when the soilheld higher(90～100%) or lower water (40～50%), and in August the death ofsegregating branch appeared.The growth of laminae was also impacted, the quantityor length of laminae, even shorter than 80cm. Finally with the result that the outputdecreased evidently.In June, the nutrition blooms in this time, the growth ofsegregating branch of Sabaigrass was affected by the field waterlogging, the caudexand segregating branch were half less than in the normal condition.

如果土壤含水量过高(90～100％)和过低(40～50％)，龙须草整个生育期没有分蘖快速增长的现象，进入8月份，甚至还出现分蘖死亡的现象，叶片的生长也受到很大影响，叶片总数极显著地减少，叶片的长度缩短，长于或等于80cm的叶片根本没有，最终导致产量显著降低；尤其是在6月份龙须草营养旺盛生长的时期，大田淹水极大地影响龙须草的生长，抑制分蘖的发生，收获时龙须草的总茎蘖数只有正常的一半。

The effects of safener on the growth and ALS activity were also examined. It showed that low concentration of AE F107892 could stimulate the growth of s-18 cultivars, but had little effect on the r-6 cultivars. High concentration of AE F107892 could inhibit the growth of both of the two cultivars.

测定了安全剂对小麦生长及ALS活力、GSH含量和GST活性的影响，结果表明：低浓度安全剂刺激敏18号小麦的生长，对抗6号的影响则较小，高浓度则都抑制了小麦幼苗的生长。

Additionally, the water fraction from wood extract can inhibit the root growth on Pak-choi. At 1000 μg/mL, after 72 h, the length ratio of root to shoot was 0.69, and the dry weight of Pak-choi root was only the 20.6% of control. But the extracts of Sapindus mukorossi have no influence on the growth of Pak-choi with normal growth 7 days.

此外，木材抽出物之水分离部则具有抑制胚根生长的作用，经浓度1，000 μg/mL处理72 h之小白菜，胚根与胚茎长比值为0.69，且其胚根乾重仅为对照组的20.6%，但无患子抽出物对正常生长7天后之小白菜并无显著抑制生长作用。

The results of studying the effects of several plant growth regulating substances on pollen germination and tube growth in pear (Pyrus serotina Rhed.) by cultivating pollens in liquid media showed that GA3,TRIA,IAA and 2,4D stimulated pollen germination and tube growth when pollen was cultivated in their lower concentration media.

采用花粉液体培养法研究了植物生长调节物质对梨花粉萌发和花粉管生长的影响，结果表明：较低浓度的赤霉素、三十烷醇、吲哚乙酸及2，4-D均能促进花粉萌发和花粉管生长，而超过一定浓度时却起抑制作用，最适宜于花粉萌发和花粉管生长的赤霉素浓度为50～300mg/L，三十烷醇为3～100mg/L，吲哚乙酸为5～25mg/L，2，4-D为5～10mg/L。

To investigate the significance of serum growth hormone,serum insulinlike growth factor1(IGF1) and urinary growth hormonelevels in the diagnosis of short statured children.

目的探讨血清生长激素、胰岛素样生长因子1(IGF1)及尿GH检测对矮小儿童诊断的意义。

The succedent implications are positive from a long-term perspective, and the reform will help to improve the exchange rate formation mechanism and the initiative and flexibility of monetary policy, change the growth of economic depending on external demand too much, be easy to correct the imbalances in global economic growth, and make positive contribution to world economic growth.

改革之后，随之而来的影响从长期来看是积极的，人民币汇率形成机制的改革将有利于完善中国汇率形成机制，提高货币政策的主动性和灵活性，改变过分依赖外需的经济增长，同时也易于校正全球经济增长的不平衡，对世界经济增长做出积极贡献。

The results indicated that the fragments (HF3～HF8) with higher MW (≥3000) but not those (HF1, HF2) with lower MW (＜3000) had effects on CFU-MK growth. Moreover, HF3～HF8 could act synergically with TPO(10ng/ml) in promoting CFU-MK growth, but HF1, HF2couldnot. In addition, there were no differences in the effects ofHF3～HF8 on CFU-MK growth, suggesting that the synergy of heparin with TPO was associated with its MW, but not its sulfate content or activity of anticoagulation.

结果表明分子量较小的HF1和HF2(＜3000)没有促进CFU-MK生长作用，而分子两较大的HF3～HF8具有促进作用，并且前2者没有协同TPO(10ng/ml)的作用，后6者都有协同作用，后6者之间的作用比较没有差异，提示肝素协同TPO的作用与其分子量有关，而与硫酸基团含量和抗凝活性无关。

Chromatic plastic film and light conversion film has been applied in protected cultivation, and plastic films with different light quality may serve as a non-chemical approach regulating growth and thermoperiodic responses of plants. A few studies showed that light quality affected growth and yield of strawberry, but little is known about its effects on photosynthesis and chlorophyll fluorescence in strawberry leaves, and fruit quality. So it is beneficial to scientific regulating growth and fruiting for strawberry protected cultivation to study on those effects and the interactions between CO2 and N supply on photosynthesis.

而低氮影响植株生长、叶片黄化，进而影响光合作用，导致抗性减弱和产量品质下降；③CO_2供应问题：目前我国草莓栽培常在设施环境下进行，在封闭性强的设施中空气流通不畅，常导致设施内部CO_2供应不足而影响光合作用，进而影响产量和品质；④光不足与光质问题：设施栽培中，光通过玻璃和塑料棚膜后使设施内的光强与光质均发生改变，这些变化特别是光质变化对草莓生长与结果的影响程度尚无明确认识。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。