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Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

To explore the relationship between T cells and aging. Methods 15 mice were divided into three groups: the 2month, the 6 month and the 15month groups. Splenocyte suspensions were prepared in sterile conditions.

目的 分析小鼠脾细胞中CD4+ CD25+ Foxp3+及CD4+ CD25- Foxp3+调节性T细胞随小鼠年龄增长的变化，探讨调节性T细胞与衰老的关系。

156 cases of pediatric outpatients with ostium praeputiale stenostomia, smegma praeputii residual and acrobystia-balanus accretion collected from January of 2005 to October of 2007 were randomly divided into two groups, i.e. MEBO group and erythromyein ointment group. Both groups were treated with surgeries of ostium praeputiale distention, smegma praeputii clearance, and acrobystia-balanus syneehiolysis, and then post-surgery pain was compared by visual analongue cale in the three time periods after surgeries. The observation of recovery and aerobystia re-accretion was taken over the three months following visits post surgery.

2005年1月～2007年10月门诊156例包皮口狭窄、包皮垢残留及包皮龟头粘连症的儿童，随机分为湿润烧伤膏治疗组及红霉素软膏对照组，均采用包皮口扩张、包皮垢清除及包皮龟头粘连分离术治疗，术后疼痛采用视觉类比量表(Visual analongue cale， VAS)对两组术后3个时间段疼痛程度进行对比，对比痊愈情况，同时术后观察三个月，包皮是否再粘连。

The facts indicate that apart from the asymmetric induction of chiral diamine backbone,sterically hindered substitute groups on the aromatic rings,and the presence of sp~3-hydridized amino donors and its N,N\'-disubstituted groups in chiral SalanCrX complexes all play important roles in controlling polymer stereochemistry and enantioselectivity.

研究发现除了手性二胺骨架的不对称诱导作用，苯环上的大位阻取代基、N，N\'-二取代基的位阻作用以及N原子上sp~3杂化的供电子效应都对调控聚合物的区域和对映体选择性有很大作用。

Methods: 480 patients were double-blind and stochastical divided into two groups, benazepril and alprostadil were uniformity given to take orally and intravenous drip to the treatment group and the comparison group. on this foundation, jinqi capsules were given to the treatment group, and compared micro-albuminuria with medicine around that two groups of patient to use the variety medicine after three months.

将早期dn患者480例双盲随机分为治疗组、对照组，两组病人均给予贝那普利口服，前列地尔静脉滴注，治疗组予以金芪胶囊口服，3个月后，比较两组病人尿微量白蛋白的变化。

Methods Eighty Class Ⅱ isogeneic F344 rats, weighing 250-350 g, were randomly divided into 2 groups(n=40). The control group adopted the Strome model. The allograft used for the experimental group preserved ascending pharyngeal artery, which formed a complex allograft together with tongue base, larynx and pharyngolarynx. End-to-end anastomosis was performed between both allograft common carotid arteries and anterior neck veins. The patency rate and survival rate of the allograft of the 2 groups were compared.

80只Ⅱ级近交系F344大白鼠，体质量250~350 g，随机分为2组，每组40只，共20对对照组采用Strome喉移植模型，实验组在切取供体喉时保留咽升动脉，将舌根、喉、喉咽作为复合移植体，供体大鼠双侧颈蓉动脉分别与受体大鼠颈总动脉、颈前静脉进行端端吻合重建移植喉血液循环，比较两组术后动静脉通畅率及移植喉存活率。

24 New Zealand white rabbits were divided into control group,Saline group and Mailuoning group,on the latter two groups the models of Hemifacial spasm were made by the temporal superficial artery closely contacting the main trunk of facial nerve at stylomastoid foramen.From the 5th week,the Saline and Mailuoning were injected intravenously into ear margin for 2 weeks on Saline and Mailuoning group respectively.At the 7th week,the MDA and SOD in serum were measured,meawhile the microstructure and ultrastructure of facial nerve were observed on 3 animal groups.

将24只新西兰大白兔随机分为对照组、脉络宁组和生理盐水组，在手术显微镜下分离出其茎乳孔处的面神经主干和颞浅动脉，使二者密切接触，以制备半面痉挛动物模型；术后第5周起对脉络宁组和生理盐水组分别自耳缘静脉推注脉络宁注射液和生理盐水持续2周；第7周取3组动物血清测定丙二醛和超氧化物歧化酶，并处死取材行面神经光、电镜观察。

Results Compared with normal group and sham operated control groups, BrdU positive and Nestin positive cells were increased in the hippocampus, dentate subgranular zone and subependymal zone in operated groups after 24 hours of reperfusion of omni-cerebral ischemia.

结果 脑缺血再灌流24 h后，海马、齿状回和室管膜下区的BrdU阳性细胞和Nestin阳性细胞增多，7～10 d达到高峰，术后20 d仍有表达；在室管膜下区，BrdU阳性细胞和Nestin 阳性细胞有向皮质、海马迁移的现象。

Results compared with normal group and sham operated control groups, brdu positive and nestin positive cells were increased in the hippocampus, dentate subgranular zone and subependymal zone in operated groups after 24 hours of reperfusion of omni-cerebral ischemia. the expression of brdu and nestin reached a peak 7-10 days after the operation, and could still be detected 20 days after the operation. the migration of brdu positive and nestin positive cells to the cerebral cortex and hippocampus was observed in subependymal region.

结果 脑缺血再灌流24 h后，海马、齿状回和室管膜下区的brdu阳性细胞和nestin阳性细胞增多，7～10 d达到高峰，术后20 d仍有表达；在室管膜下区，brdu阳性细胞和nestin 阳性细胞有向皮质、海马迁移的现象。

Methods All cases were divided into two groups:medical obturation glue group, which were used medical glue in hepatolobectomy of APLD from March 2002 to December,2005;Controlled group, which were not used medical glue from January,1995 to February,2002.Abdominal drainage amount, reserved drainage tube time, subphrenic infection were compared between the two groups.

将所有病例分成两组，2002年3月～2005年12月应用医用创面封闭胶在APLD肝叶切除中病例设为A组，1995年1月～2002年2月未用医用创面封闭胶于肝叶切除APLD病例进行对比研究，比较应用创面封闭胶处理肝断面和不用创面封闭胶的两组病例腹腔引流量、带管时间以及膈下感染发生情况。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。