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The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

From the angle of the dialectical relation of difference and identity, connecting further peoples real patterns of expression (the pattern of expression of the category, the pattern of expression of groups, the pattern of expression of the individuals), we may make the essence of human being to be particularized to three aspects of essence of the category, the groups and the individuals, these three aspects not only prescribe intrinsically the corresponding patterns of expression ofjustice (the justice of the category, the justice of the groups, the justice ofthe individuals), but also prescribe intrinsically the corresponding contents of development and ways of realization of justice.

从差异与同一的辩证关系角度,再结合人的现实的表现样态(类的表现样态、群体的表现样态、个体的表现样态),我们可以把人的本质具体化为类本质、群体本质、个体本质三个方面,这三个方面不仅内在地规定着相应的正义的表现形态(类的正义、群体的正义、个体的正义),而且内在地规定着相应正义的发展内容和实现方式。

Using finite fields , the classical groups give rise to finite groups , intensively studied in relation to the finite simple groups ; and associated finite geometry , which has both combinatorial and algebro-geometric sides.

利用有限领域,古典的群体产生有限的群体,深入研究有关的有限简单群体;及相关有限几何,这两个组合和algebro几何双方。

Second, in the fourth character, we discuss Vague groups, give some interesting properties, and the most important result is the homomorphism and isomorphism between two L-Vague sets, the homomorphism and isomorphism theorems in the theory of classical groups are extended to the L-Vague groups.

二是研究了Vague集的群问题,给出了一些性质,并着重研究了L-Vague群的同态和同构,将群中的同态定理和同构定理推广到L-Vague群上,见第四章。

Furthermore,we consider the relations between the general linear groups and the corresponding BN-pairs and show that they satisfy a commutative diagram of groups.In Chapter 2,we study the structure of unitary groups over finite group rings R and its applications.

第一章考虑任意局部环上典型群的BN-对问题,构造了局部环上一般线性群、辛群、正交群的BN-对,并且证明了局部环上一般线性群与对应的BN-对之间满足群交换图关系。

Through interviewing 3 different adulthood subjects with the open question method and subjects rating short scale items, the results showed that (1) all 3 age groups (young adults, middle-aged adults, and older adults) responded and rated mutual considerateness and tolerance as the most important elements for maintaining marital relationships;(2) all 3 age groups shared the belief that mutual respect, trust, encouragement, concern, understanding, care, and thanksgiving and responsibility were also important elements for maintaining marital relationships; and (3) all 3 age groups rated shared interest, activity, discussion topic, belief and value, and occupied time and personal space as the least important elements for maintaining marital relationships.

经由对三不同成人期已婚受试以开放式问题访问与受试在简短量表的回答,本研究结果显示彼此体谅、容忍是各年龄组受试认为是维系婚姻关系最重要的因素。彼此尊重、信任、鼓励、关心、了解、有责任感、相互照顾彼此感恩是各年龄组受试所共同认为是维系婚姻关系较重要的因素。有共同的兴趣、活动、话题、信仰、价值观与拥有各自的生活空间与时间是各年龄组所共同认为是维系婚姻关系较不重要的因素。

One hundred and twelve in-patients undergoing cholecystectomy were randomly divided into two groups,experimental groups and contradistinctive groups.

选取胆囊切除术患者112例,随机分为实验组和对照组各56例。

RESULTS:The increase in appetite,physical strength,and body weight were observed in groups of 6,12,24 mg dosage.Dry mouth was a general phenomenon in groups of 36mg and above dosage and dry throat,sore throat and dry eye were occurred in some of them but they are tolerable and relieved with continuous treatment.Dizzy,tinnitus,oppressed feeling in the chest,dreamfulness,and oral ulcer were occurred in a few volunteers in groups of above 60 mg dosage with one case of increase in ALT and one case of increase in ALP.

结果:小剂量(6,12,24 mg组有增加食欲、体力和体重的作用;36 mg以上组(包括36mg口干现象十分普遍,部分志愿者出现咽干、咽痛、眼干等症状,但均能耐受,且随服药天数的延长而自行缓解。60 mg以上组个别志愿者出现头晕、耳鸣、胸闷、多梦、口腔溃疡等症状,受试者中有1例ALT升高,1例ALP升高,个别受试者鼻中有血丝或血痂。

METHODS: Forty rats were randomly into endocrine and exocrine groups, and rats in the two groups were sub-grouped into control and experimental groups with 10 in each group.

随机将40只大鼠均分至内分泌和外分泌2个平行实验中,各分为对照组10只和实验组10只。

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推荐网络例句

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作: 1 回顾了直线电机发展历史,电机的驱动技术演变,特别是针对直线电机的驱动,简要介绍了直线感应电机驱动电梯的优点和不同结构类型,对传统的v/f控制,基于空间矢量法的v/f控制,矢量控制,和基于电压空间矢量的直接转矩控制进行了比较,确立了本课题的研究主题:直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用,并详细阐述了注浆技术的施工流程。

I knew nor shyness nor fear, my life was boisterous.

我不懂得羞怯和惧怕,我的