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So we selected the colchicine optimumparameters were 0.2μg/ml and 6 hour cultured duration.The third, there were significantly differences percent between 10 hourgroups and other three groups (4 hour groups, 6 hour groups and 8 hour groups) in0.1μg/ml groups; and there were significantly differences between 8 hour groupsand 10 hour groups, but they had no significantly differences among other twogroups in 0.3μg/ml groups; and there were significantly differences between 10hour groups and 6 hour groups, but 10 hour groups had no significantlydifferences among other two groups in 0.5μg/ml groups; and there weresignificantly differences between 8 hour groups and other three groups in0.7μg/ml groups on the metaphases (P＜0.05), in the experiment of the effect onthe mouse 4- cell embryo stage single blastomere of vinblastine with differentconcentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.2μg／ml和6小时。3、在确定长春花碱不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著(4小时组、6小时组和8小时组)；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与6小时组差异不显著，而与其他两组差异显著；阻断培养0.7μg／ml浓度组中8小时组与其他三组差异显著(P＜0.05)。

Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.

对荷W256大鼠，生兴克癌冲剂20、10、5 g / kg三组的平均瘤重明显低于对照组(P.01，P.05)，生兴克癌冲剂20、10 g / kg组的平均肿瘤抑制率均大于32 %；⑵与空白对照组相比，生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01)，生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上；⑶与S180荷瘤组相比，生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势，其中生兴克癌冲剂5 g / kg组对荷瘤小鼠的胸腺指数有一定的提高作用(P.05)；⑷与S180荷瘤组相比，生兴克癌冲剂20、10 g /kg组可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01)，表明其可提高荷瘤小鼠体液免疫功能；⑸与对照组相比，生兴克癌冲剂20 g /kg组可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01)，表明其可提高荷瘤小鼠细胞免疫功能。

The results were as follows:The first, there were significantly differences percent between 0.6μg/mlgroups and other three groups (0.2μg/ml groups, 0.4μg/ml groups and 0.8μg/mlgroups) in 2 hour groups; and there were significantly differences between0.4μg/ml groups and other three groups in 4 hour groups; and there weresignificantly differences between 0.6μg/ml groups and other three groups in 6hour groups; and there were significantly differences between 0.4μg/ml groupsand other three groups in 8 hour groups on the metaphases (P＜0.05), in theexperiment of the effect on the mouse 4- cell embryo stage single blastomere ofcolchicine with different concentrations and duration, by x~2 statistical analysis.

实验结果如下：1、在确定秋水仙素不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中0.6μg／ml浓度组与其他三组差异显著(0.2μg／ml组、0.4μg／ml组和0.8μg／ml组)；阻断培养4小时组中0.4μg／ml浓度组与其他三组差异显著；阻断培养6小时组中0.6μg／ml浓度组与其他三组差异显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

So we selected thevinblastine optimum parameters were 0.1μg/ml and 8 hour cultured duration.The fourth, there were significantly differences percent between 10 hourgroups and other three groups in 0.1μg/ml groups; and there were no significantlydifferences between 8 hour groups and 10 hour groups, but they had significantlydifferences among other two groups in 0.3μg/ml groups; and there weresignificantly differences between 10 hour groups and other three hour groups in0.5μg/ml groups; and there were significantly differences between 10 hour groupsand other three groups in 0.7μg/ml groups on the metaphases (P＜0.05), in theexperiment of the effect on the mouse 8- cell embryo stage single blastomere ofvinblastine with different concentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg／ml和8小时。4、在确定长春花碱不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.7μg／ml浓度组中10小时组与其他三组差异显著(P＜0.05)。

Sowe selected the colchicine optimum parameters were 0.6μg/ml and 6 hour culturedduration.The second, there were no significantly differences between the four groupsin 2 hour groups; and there were significantly differences between 0.2μg/ml groups and other three groups in 4 hour groups; and there were no significantlydifferences between the four groups in 6 hour groups; and there were significantlydifferences between 0.4μg/ml groups and other three groups in 8 hour groups onthe metaphases (P＜0.05), in the experiment of the effect on the mouse 8-cellembryo stage single blastomere of colchicine with different concentrations andduration by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.6μg／ml和6小时。2、在确定秋水仙素不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中四个浓度组差异均不显著；阻断培养4小时组中0.2μg／ml浓度组与其他三组差异显著；阻断培养6小时组中四个浓度组差异均不显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

Results: For CRF rats which made by fed with feedingstuff contains 0.75% of adenine for 4 weeks, the weight of model control group rats were lighter than that of Yi Shen Granule groups rats. The water wastage of Yi Shen Granule groups rats were less than that of model control group rats. Compared urine volume, the ejectable quantity of Na, K, Cl and proteinuria in 12 hours , the data of Yi Shen Granule groups rats were less than that of model control rats. The specific gravity of Yi Shen Granule groups rats was higher than that of model control rats. The numerical value of RBC, HGB, HCT, MCV, MCH in model control group was lower than that in Yi Shen Granule groups. The content of BUN and Cre in serum of model control groups rats were higher than that of Yi Shen Granule groups. The results of excretion test of phenolsulfonphthalein showed that the RPF of Yi Shen Granule groups rats was larger than that of model control group rats.

结果： 益肾颗粒可以使0.75%腺嘌呤饲喂法致大鼠慢性肾衰模型动物体重增长加快；给药后观察，给药组动物饮水量较少，12小时尿量、12小时钠、氯、尿蛋白排泄总量均显著低于模型组动物，尿液比重值较大；模型组动物的RBC、HGB、HCT、MCV、MCH等指标的数值较给药组动物的数值小；给药组动物血清中BUN和Cre的含量较低；给药组动物酚红排泄率均高于模型组动物；给药30天，处死各组动物，尸解观察，模型对照组动物肾脏呈灰白色，各给药组动物肾脏表面均出现不同程度的红、白相兼的颗粒状纹理，各组动物肾脏均肿大，切面可见不同程度的腺嘌呤结晶沉积。

Examples of metal chelating groups include hydroxides especially aryl or heteroaryl hydroxides such as phenolic hydroxides; amines which may be aliphatic aryl or heteroaryl; mercaptans which may be aliphatic aryl or heteroaryl; carboxylic acids which may be aliphatic aryl or heteroaryl; oximes and ketoximines; acetarylamides; hydroxy silanes and silicones; N-containing heterocycles such as imidazoles benzimidazoles triazoles benzotriazoles thiazoles isothiazoles acid anhydrides and more preferably acid groups (especially carboxylic acid groups phosphoric acid groups polyphosphoric acid groups phosphonic acid groups sulphuric acid groups and sulphonic acid groups).

金属螯合组的例子包括氢氧化物，特别是芳基或如酚醛氢氧化物杂氢氧化物；胺可能是脂肪族，芳基或杂；硫醇可能是脂肪族，芳基或杂，这可能是脂肪族，芳基或羧酸杂；肟和ketoximines； acetarylamides；羟基硅烷和有机硅，含N，如咪唑，苯并咪唑类，三唑类，苯并三氮唑，噻唑类，isothiazoles，酸酐杂环，多最好酸组（特别是羧酸团体，磷酸，团体，多聚酸组，膦酸组，硫酸团体和磺酸组）。

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示，未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色，核区较暗；CDDP及β-Ele处理组细胞为暗绿色，其中可见皱缩的凋亡细胞；THP处理的细胞胞质荧光弱呈灰绿色，由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和，胞核被染成桔红色，结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

The hydroxyl and carboxyl, belonging to auxochromic groups, were oxidized into the carbonyl and ester groups, belonging to chromophoric groups, during inducing discoloration under the pretreatment conditions, which makes the wood color deeper. There were plenty of auxochromic groups and chromophoric groups in the acetone extractives of silver chain. The interaction between the auxochromic groups and the chromophoric groups made the wood color deeper.

结论］羟基、羧基等助色基团在预处理条件下氧化成羰基、酯基等发色基团，使木材的颜色加深；刺槐木材丙酮抽出物的主要成分中含有大量发色基团和助色基团，这些基团间的相互作用使木材颜色加深。

3NE、MTT、LDH、ET-1、ICAM-1 and Tie-2experiment show: in the process of oxygen glucose deprivation/reoxygenation ,BMEC have subjected great damagement, I/R groups have significant differences compared with NC groups (p.05);polymorphonuclear leukocyte can aggregrate this injury, I/R+PMN groups have significant differences compared with I/R groups (p.05); after Sivelestat Sodium treatment, the effect of I/R injury has been decreased, I/R+PMN+Siv groups have significant differences compared with I/R+PMN groups (p.05), and NE、MTT、LDH experiment show that the three dose groups there was a significant dose-effect relationship.

3测定NE、MTT、LDH、ET-1、ICAM-1、Tie-2 mRNA均显示：氧糖剥夺/复氧复糖过程能对BMEC造成损伤，I/R组与NC组比较有明显的统计学差异(p.05)；中性粒细胞能加重对BMEC的损伤，I/R组与I/R+PMN组之间比较亦有统计学差异(p.05)；西维来司钠处理后，血管内皮细胞的损伤明显减轻，I/R+PMN组与I/R+PMN+SIR组比较有统计学差异(p.05)，并且NE测定以及MTT、LDH实验提示在西维来司钠三个剂量组之间存在明显的量-效关系，其对BMEC的保护作用有剂量依赖性。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。