查询词典 grouper

Effects of dopamine and apomorphine on the release of GH and the level of GH mRNA were analyzed in a static incubation system for pituitary fragments of orange-spotted grouper.

应用静态孵育系统研究了多巴胺和DA激动剂阿扑吗啡对斜带石斑鱼脑垂体GH释放和GH mRNA合成的影响。

I can not sleep in bed, imagine the sea grouper and barracuda.

我在床上无法入眠，幻想着海中的石斑鱼和梭鱼。

The farm raises barramundi, a new fish farmed as a replacement for grouper, according to the Web site for Australis Aquaculture, an Australia-based company.

澳洲「南极光水产养殖」公司在网站中指出，他们不养殖石斑鱼而改养龙鱼。

Protein sequence of snakehead IgH has 55%, 50%, 45%, 36% and 28% identical to orange-spotted grouper, blackfin icefish, bastard halibut, rainbow trout and zebrafish IgH, respectively.

序列相似性比较显示乌鳢IgH与点带石斑鱼、黑鳍冰鱼、牙鲆、虹鳟的相似性依次为55%、50%、45%和36%，与斑马鱼类的相似性较低（为28%）。

Including bass, halibut, swordfish, red snapper, ahi, bluefish, grouper, and trout.

包括低音，比目鱼，旗鱼，红快， ahi ，下来，石斑鱼，鲑鱼。

Hovering over a bed of brain coral, a coney grouper regards the OceanEye camera used to photograph it.

一条蹄兔鲶科鱼注视着"海洋眼照相机"经常的拍它的照片。

Huge viewports are filled with flashing shoals of grouper, wrasse, amberjack, conger eel and, finally, a huge ray cruising the turquoise depths imperiously.

巨大的观景窗外充斥着闪闪发光的鲶鱼群、濑鱼、琥珀鱼、海鳗，最后，一道巨大的光线透过深深的松石绿色的海水。

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58％(n=6) and 5. 69％(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体，羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法，该系统的灵敏度为0.412ng GH／ml(n=6)，组内变异系数为2.58％(n=6)，组间变异系数为5.69％(n=6)，斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行，金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行，表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性，灵敏度达到了测试血清样品中GH的水平。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。