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Result The inhibitory effect on pulmonary metastasis of transplanted tumor were 46.36%, 32.73%, 18.2% in the Runing granule preparation group, warming kidney group, dispersing liver and promoting blood circulation group respectively. There wasn't significant difference between Runing granule preparation group and CTX group (50.91%). The expression of VEGF was dropped in Runing grannie preparation group and dispersing liver and promoting blood circulation group. The expression of TIMP-1mRNA was up-regulated in Runing Granule group and warming kidney group. The expression of MMP-9 was dropped in Runing granule preparation group and warming kidney group.

乳宁冲剂组及拆方温肾组、疏肝活血组对裸鼠乳腺癌移植瘤的肺转移抑制作用分别为46.36%、32.73%、18.2%,乳宁冲剂组作用与CTX组(50.91%)比较无显著差异;而乳宁冲剂组、疏肝活血组均可下调肿瘤VEGF蛋白表达;乳宁冲剂全方、温肾方可明显使TIMP-1mRNA的表达升高,MMP-9表达下降;对MMP-9/TIMP-1蛋白表达比值影响以温肾组下降最明显。

Three hundred and twenty-four crossbred (Landrace×Yorkshine) barrows with average body weight of 27 kg were randomly assigned to 6 dietary groups: group Ⅰ(chlorotetracycline group, added 50 mg/kg chlortetracycline in basal diet); group Ⅱ(origanum oil group, added 20 mg/kg oreganum oil in basal diet); group Ⅲ(probiotics group, added 1.5 g/kg probiotics in basal diet); group Ⅳ(nutrient enhancement group, elevated dietary vitamin E, vitamin B family and micro elements Zn, Mn, I); group Ⅴ(origanum oil+nutrient enhancement group); group Ⅵ(probiotics+nutrient enhancement group).

试验选用平均体重27 kg,健康长×大杂交阉公猪324头,随机分为6组:Ⅰ组(金霉素组,在基础日粮中添加50 mg/kg金霉素)、Ⅱ组(牛至油组,在基础日粮中添加20 mg/kg牛至油)、Ⅲ组(益生素组,在基础日粮中添加1.5 g/kg益生素)、Ⅳ组(营养调控组,提高日粮中维生素E和B族维生素、微量元素Zn、Mn和I水平)、Ⅴ组、Ⅵ组。

The three groups were loaded and analyzed under the same loading according to the mean value of the bite force of incisors. RESULTS:(1)The maximum stress of dentin in group A was twice higher than that in group B. The maximum primary stress, minimum primary stress and maximum shear stress of group A were respectively 236.35, 228.83 and 218.05 percent of those in group B. The difference of maximum stress values of group B and group C was neglectable.(2) The maximum stress distribution of dentin in group A and group B was quite different (the stress was concentrated in labial and lingual side of the cervical dentin in group A, otherwise in group B it was concentrated in the area around the alveolar and the labial and lingual side of the dentin which was opposite to the tip of the cast metal post and core). The maximum stress distribution of dentin in group A and group B was almost the same.

结果:(1)从牙本质的应力大小来看,A组中牙本质最大应力值比B组中牙本质最大应力值增大了1倍以上(最大主应力、最小主应力和最大剪切应力A组分别是B组的236.35%、228.83%和218.05%),而B组与C组的牙本质最大应力值相差极小;(2)从牙本质的应力分布位置来看,A组与B组的牙本质最大应力分布位置相差甚远(A组主要集中在牙颈部唇舌侧肩台部的牙本质上,B组主要集中于牙槽嵴顶附近及铸造桩核尾端相对应的唇舌侧的牙本质上),而B组与C组的牙本质最大应力分布几乎在同一位置。

To date, the Group has successfully become the Wal-Mart, Budweiser, Tsingtao Brewery, China Resources Blue Sword, Coca-Cola, Pepsi, unified enterprise, Ting Hsin Group, Wahaha Group, Pacific Insurance, China Merchants Bank, Construction Bank of China, Haier Group, Changhong Group, Jialing Group, Zongshen Group, Hongta Group, Stentor Group, New Hope Group, Youngor Group, Taiji Group, Sunshine 100 Real Estate Group and many other well-known enterprises, umbrella-type suppliers.

迄今为止,已成功成为沃尔玛集团、百威啤酒、青岛啤酒、华润蓝剑、可口可乐、百事可乐、统一企业、顶新集团、娃哈哈集团、太平洋保险、招商银行、建设银行、海尔集团、长虹集团、嘉陵集团、宗申集团、红塔集团、宏声集团、新希望集团、雅戈尔集团、太极集团、阳光100地产集团等众多知名企业的伞类供应商。

24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组无显著性差异(p>0.05)。本实验结果发现,与单纯缺血12小时、24小时大鼠相比,药物干预组缺血侧大脑皮层TGF-β1免疫反应明显增强,计算机辅助图像半定量分析两组间缺血侧大脑皮层TGF-β1免疫阳性细胞数差异非常显著。说明药物对局灶性脑缺血损伤可能有一定保护作用,其机制可能与诱导脑细胞TGF-β1合成增加有关。然而,药物调控TGF-β1表达,其促TGF-β1表达增强的机制可能是这样的:脑缺血时TGF-β1mRNA表达增加可能与缺氧诱导神经和胶质细胞刺激生长因子表达有关。

METHODS: 40 rats were randomly divided into four groups:normal group, asthenic spleen group, high-dosage group and low-dosage group with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR.

40只大鼠随机分为空白对照组、脾虚组、桂枝汤大剂量组和桂枝汤小剂量组等4组,每组10只;用规范的脾气虚证大鼠模型造模方法将B组、C组、D组大鼠造模,B组、C组、D组分别用蒸馏水、桂枝汤大剂量、桂枝汤小剂量灌胃,标本采集后用RT-PCR检测NFAT mRNA,IL-4 mRNA和IFN-γ mRNA表达水平。

Results l.Serum concentrations of VCAM-1 in PIH (93.39 + 57.3)ug/ml were higher than that in normal pregnant group. Serum concentrations of VCAM-1 in moderate(97.89 + 34.07)ug/ml and severe PIH(132.24 + 60.97)ug/ml were significantly higher than that in normal group.(P.05, P.01). There was no difference between serum levels of VCAM-1 mild PIH and normal pregnant group. 2.Serum levels of IL-6 in PIH group(102.17 ?48. 31)pg/ml were significantly higher than that in normal group(49.16 + 12.9)pg/ml.Serum levels of IL-6 in moderate(95.79+31.19)pg/ml and severe PIH( 127.27+11.3 8)pg,ml were significantly higher than that in normal group. There was no difference between serum levels of PIH in mild PIH (52.13 + 12.90)pg/ml and normal pregnant group. 3. In PIH group, serum concentrations of VCAM-1 correlate with the levels of IL-6,r=0.63. 4.The expression of VCAM-1 in cytotrophoblast of spiral arteries in normal pregnant group(100%) were significantly higher than that in PIH group.Theexpression of VCAM-1 in moderate PIH(37.50%) and sever PIH were lower than that in normal group.There was no difference between the mild PIH and normal group.Conclusions The increased levels of serum of VCAM-1 may participate in the process of vascular endothelium damages in PIH.

结果 1、妊高征组血清VCAM-1浓度为(93.39±57.3)μg/ml明显高于正常妊娠组(44.87±15.60)μg/ml,差别有显著性(P<0.05);中、重度妊高征组VCAM-1浓度分别为(97.89±34.07)μg/ml和(132.24±60.97)μg/ml,与正常妊娠组比较,差异有显著性(p<0.05)和非常显著性(P<0.01);轻度妊高征组VCAH-1为(48.46±15.60)μg/ml与正常妊娠组比较,差异无显著性(P>0.05)。2、妊高征组血清IL-6含量为(102.17±48.31)pg/ml,明显高于正常妊娠组(49.16+12.9)pg/ml,差异有非常显著性(P<0.01);中、重度妊高征组IL-6含量分别(95.79±31.19)pg/ml和(127.27±11.38)pg/ml,与正常妊娠组比较,差异有显著性(P<0.05)和非常显著性(P<0.01);轻度妊高征组IL-6含量为(52.13±12.90)pg/ml与正常妊娠组比较,差异无显著性(P>0.05)。3、VCAM-1与IL-6水平呈明显正相关,r=0.63(P<0.01)。4、子宫胎盘床螺旋动脉滋养细胞VCAM-1表达,正常妊娠组都存在阳性表达(阳性表达率100%),妊高征组有10例阳性表达阳性表达率为叩们,差别有非常显著性河<0.01L 中、重度妊高征组阳性表达率分别为37、50%和0,与正常妊娠组比较,差别有显著性河<0.05)和非常显著性汀<0.01太轻度妊高征组阳性表达率为83.33凡与正常妊娠组差别无显著性问>0.05)。

Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.

二整体动物水平上探讨心肌有效转染途径:以PLacZ为报告基因,将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入);转染质粒心包腔内注射组及其阴性对照组:质粒+微泡+酶的混合物心包腔内注射,超声导入;转染质粒舌下静脉注射组及其阴性对照组:质粒+微泡混合液经舌下静脉注射,超声导入。

Methods: Wistar rats were divided into normal group, model group, TCM group Ⅰ, TCM group Ⅱ and western medicine group. In addition to the normal group, the other four groups were reproduced animal model of pulmonic fibrosis. The model group were given normal saline gavage, The TCM group Ⅰ given Feifltkang Mistura (1g/ml) gavage, The TCM group Ⅱ were given Fe4fukang Mistura (0.5g/ml) gavage, WM group were given prednisone gavage. Half of each group were taken blood to test in 28th, 56th day.

选择Wistar大白鼠随机分为正常组、模型组、中药Ⅰ组、中药Ⅱ组、西药组,除正常组外,其余四组均复制肺纤维化动物模型;模型组用生理盐水灌胃,中药Ⅰ、Ⅱ组分别用高、低剂量肺复康合剂灌胃,西药组用强的松灌胃;各组分别检测血清相关指标。

All the indexes were tested in 2 -. 4 .. 6 weeks of operative intervals respectively. Results:(1) The general observation in the experimental group ,such as spirit, hair, body weight and activity ,was superior to that in the control group.(2) The recovery rate of sciatic nerve functional index (SF1) was statistically significantly higher in the experimental group than in the control group .(3) electrophysciological indexes: m the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon, thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organell a and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在木后第2、4、6周时进行各项指标的检测,结果发现:(1)实验组SD大鼠在整个实验过程中,其精神、毛发、体重,运动灵活性等一般情况优于对照组;(2)实验组的坐骨神经功能指数恢复率显著优于对照组,P<0.01;(3)神经电生理指标:实验组运动神经诱发电位潜伏期的延迟率显著优于对照组,P<0.05;复合肌肉动作电位振幅的恢复率显著高于对照组,两组比较第2周时P<0.01,第4、6周时,P<0.05;小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组,P<0.01;(4)小腿三头肌湿重的恢复率,第2、4周时实验组高于对照组,差异非常显著,P<0.01;第6周时,差异仍显著,P<0.05;(5)组织形态学指标:有髓种经轴突计数、再生轴突直径的恢复率,毛细血管面积,实验组均显著大于对照组,P<0.01、P<0.05、P<0.001;实验组髓鞘厚度的恢复率也显著高于对照组,第2周时P<0.01,第4、6周时,P<0.05;(6)透射电镜超微结构观察:实验组再生轴突中细胞器丰富,髓鞘结构成熟,神经再生情况优于对照纽。

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