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Blood were obtained for blood RT and plasma endotoxin concentration check. 6 All data underwent ANOVA analysis. Results: After 3hrs of LPS injection, 1 in hypermetabolic sepsis group, the average HR went to 334/min, the average body temperature went to 41.0℃, while in control group they were 274.9/min and 38.2℃ respectively. p=0.000, presented significant statistical differences. The average BP and breath rate in HS group were 97.1mmHg and 94.3/min , while they were 95.9mmHg and 76.4/min in control group, respectively presenting no statistical differences. 2 In HS group plasma endotoxin concentration was 0.088EU/ml while it was 0.013 EU/ml in control group presenting statistical significance, p=0.002. 3 Blood white cell ratio and total white cell counting were 82.73% and 1.90×109 in HS group, while they were 64.39% and 7.58×109 in control group presenting very significant statistical differences(p=0.001). There was no statistical significances in RBC counting and hemocrit between two groups.

结果:LPS持续灌注3h后,结果如下:1)高代谢脓毒症组的心率升高至334.0次/min、体温升高至41.0℃,而对照组则分别为274.9次/min和38.2℃,两组间有显著的统计学差异,p=0.000;血压在高代谢脓毒症组是97.1mmHg,而对照组为95.9mmHg,两组间无统计学差异;脓毒症组的呼吸加快至94.3次/min,对照组则提高至76.4次/min,两组间无统计学差异(p=0.212);2)其血浆内毒素含量为0.088EU/ml,而对照组为0.013 EU/ml,两组间有显著的统计学差异,p=0.002;3)血中性粒白细胞比值升高至82.73%,对照组则为64.39%,两组间有显著的统计学差异(P=0.001);白细胞总数则明显下降至1.90×109/ml,而对照组则为7.58×109/ml,p=0.001,两组间有显著的统计学差异;两组间的红细胞数及血细胞比容均无统计学差异。

Material and Methods:From March,2008 to January,2009,250 patients(146 Males and 104 Females,ranged from 18 to 82 years old,mean age was 48 years old) enrolled in the First Affiliated Hospital of Kunming Medical College were performed dual energy scan(Group A,200 patients) and Neuro-DSA scan(Group B,50 patients) using DSCT.The patients were randomly divided into Group A and Group B.Regarding to the justification of scan protocol,100 patients in Group A were selected as sub-group to explore the scan protocol.The image quality Was analyzed both in four categories(4.0/60、4.5/60、4.0/70、4.5/70) of flow rate and total amount of contrast-enhanced material and in three categories(100—150HU、150—200HU、200-250HU) of CT value of left common carotid artery.The comparison of two scan approach was based on image quality,radiation does, postprocessing methods,time of scanning and subtraction and data storage amount.To validate the diagnostic capability of DE-CTA virtual unenhanced images,100 patients in Group A were undergoing head scan both in routine non-contrast program and DE-CTA virtual unenhanced program.Mean CT values,signal-to-noise ratio,image quality,sensitivity of lesion identification,and radiation does were applied to comparison.According to diagnostic results of dual energy of cerebral arterial angiography,62 patients out of 200 patients in Group A were confirmed with cerebral vessel lesions,the results were complied with DSA findings(the time between the two methods were within 1 week).

材料与方法收集2008年3月到2009年1月间在昆明医学院第一附属医院行DSCT CTA检查者250例(男性146例,女性104例,年龄范围18-82岁,平均48岁),随机分为A、B两组,A组200例行双能量头颅CTA扫描;B组50例行Neuro-DSA扫描。A组前100例按对比剂不同流率和总量分为4组(4.0/60、4.5/60、4.0/70、4.5/70)对图像质量比较;按触发时左颈总动脉内平均CT位分3组(100—150HU、150—200HU、200-250HU),比较图像质量的差异;对A、B两组血管图像质量、辐射剂量、后处理的方法、扫描和后处理时间及数据的容量进行比较分析;A组中前100例行常规与DE-CTA虚拟平扫的平均CT值、信号噪声比、图像质量、病灶显示情况、辐射剂量的比较分析;A组中行DSA检查62例(两种方法间隔时间均1周内),比较两种检查方法诊断结果是否符合,并对其中24例动脉瘤行动脉瘤部位、数目、形态、瘤颈显示程度、瘤颈大小及瘤体长径和短径的比较研究。

The effect of Jiusuyu on ebriety rate, ebriety time of acute alcoholism mice observed after preventing treatment 80 healthy mice, bisexual and each half, were randomly divided into 4 groups, 20 mice every group, namely model group (equal volume physiological saline were given through the means of watering stomach), low-dose group of Jiusuyu (called low-dose group, 1.62g/100g avoirdupois Jiusuyu drank through the means of watering stomach), high-dose group of Jiusuyu (called high-dose group, 3.24g/100g avoirdupois Jiusuyu drank through the means of watering stomach), and Gehuaji-echeng liquid control group (called control group, 1.31g/100g avoirdupois) Gehuajiecheng liquid were drunk through the mea

酒速愈预防性给药对急性酒精中毒小鼠醉酒时间、醉酒率的影响健康昆明种小鼠,雌雄各半,随机分为4组:模型组、酒速愈低剂量组(灌服酒速愈1.62g生药/100g体重)、酒速愈高剂量组(灌服酒速愈3.24g生药/100g体重)、葛花解酲汤组(灌服葛花解酲汤1.31g生药/100g体重),每组20只。各组小鼠禁食12hs,各治疗组均一次性给药,模型组灌服生理盐水,30mins后均以56°红星二锅头酒灌胃(1.4ml/100g体重)。观察记录小鼠醉酒时间(从清醒到翻正反射消失的时间),小鼠醉酒与否以翻正反射是否消失为标准:小鼠灌酒后将其背向下轻轻放入鼠笼,若小鼠背

Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果:SPG-Rg3 IC50为(155.70±0.71) mg·L-1,当SPG-Rg3浓度在37.5~600.0mg·L-1时,MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大,与对照组比较,细胞增殖受到明显抑制(P.05);MCF-7细胞的生长周期也发生变化,S期细胞数增加,明显高于对照组(P.01,G2/M期细胞数则明显少于对照组(P.01;并且在G1峰前出现明显的凋亡峰,凋亡细胞数增加。

The healing rate of wound of control group is obviously higher than the model group.(3) Observe under the microscope: When 3 days after we practice the surgery, HE and observe control group wound have a large number of inflammation nature cell soak, model group wound surface inflammatory cell relatively sparse in Balb/c mouse;The control group can seeⅠtype collagenic and green type collagenic day, model group type be obviously less than the control group; When 7 days after we practice the surgery,HE and bitter acid-staining observe control group peripheral inflammation cell soak, have appeared granulation organize.

3镜下观察:创伤后第3d,HE染色观察对照组伤口有大量炎性细胞浸润,模型组小鼠创面炎症细胞较稀疏,天狼猩红染色对照组可见少量呈黄色的Ⅰ型胶原和呈绿色的Ⅲ型胶原,模型组Ⅲ型胶原要明显少于对照组;创伤后第7d, HE染色观察对照组周边炎性细胞浸润,有肉芽组织出现。

The results were compared among 4 groups. Results:①OCB positive rate was 37% in MS patients, which was lower than other reports, 24% in central nervous system infectious diseases group,5% in central nervous system non-infectious diseases group and 0 in peripheral nervous system diseases group,respectively.②OCB positive rate in conventional MS was 56%, which was higher than that in neuromyelitis optica.

结果①多发性硬化组OCB阳性率为37%,中枢神经系统感染组为24%,中枢神经系统非感染性疾病组为5%,外周神经系统疾病组为0;多发性硬化组的OCB阳性率比其他各组高,但低于国外报道的阳性率;②多发性硬化患者中传统型多发性硬化组OCB阳性率为56%,高于视神经脊髓炎组的11%。

The test group with PMSG and HCG were compared with the control group in the oestrus rate,conception rate and live farrowing rate.

试验比较了PMSG+HCG与对照组的发情率、受胎率、产仔率等繁殖性能指标。

Electrophysciological indexes: in the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon,thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organella and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在术后第2、4、6周时进行各项指标的检测,结果发现:(1)实验组SD大鼠在整个实验过程中,其精神、毛发、体重,运动灵活性等一般情况优于对照组;(2)实验组的坐骨神经功能指数恢复率显著优于对照组,P<0.01;(3)神经电生理指标:实验组运动神经诱发电位潜伏期的延迟率显著优于对照组,P<0.05;复合肌肉动作电位振幅的恢复率显著高于对照组,两组比较第2周时P<0.01,第4、6周时,P<0.05;小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组,P<0.01;(4)小腿三头肌湿重的恢复率,第2、4周时实验组高于对照组,差异非常显著,P<0.01;第6周时,差异仍显著,P<0.05;(5)组织形态学指标:有髓神经轴突计数、再生轴突直径的恢复率,毛细血管面积,实验组均显著大于对照组,P<0.01、P<0.05、P<0.001;实验组髓鞘厚度的恢复率也显著高于对照组,第2周时P<0.01,第4、6周时,P<0.05;(6)透射电镜超微结构观察:实验组再生轴突中细胞器丰富,髓鞘结构成熟,神经再生情况优于对照组。

Result:1:in the aspect of controlling of the swelling development, treatment group is distinctly superior to control group.2:in the aspect of deswelling rate,treatment group is superior to control group.3:in the aspect of controlling pain degree,shorten the applying time of the anodyne, treatment group is distinctly superior to control group.4:in the aspect of controlling the large-area tension bullae incidence,treatment group is superior to control group.

在消肿速度上,治疗组优于对照组。3。在控制疼痛程度,缩短止痛药应用时间方面,治疗组明显优于对照组。4。在控制大面积张力性水泡发生率上治疗组优于对照组。

Patients in observation group were treated by folacin,vitamin B6、B12 and patients in control group were treated by placebo.Results:The total effective rate of observation group is 75%(marked effective rate 21.9%).

我们将62例确诊为高同型半胱氨酸血症的患者随机分为观察组及对照组,观察组给予叶酸、维生素B6、维生素B12,对照组给予安慰剂,用药2周后复查血浆同型半胱氨酸水平。

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The split between the two groups can hardly be papered over.

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