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objective:to investigate the therapeutic action of somatostatin in pancreatic leakage after pancreaticoduodenectomy.methods: to collect 100 patients with pancreaticoduodenectomy during 2003～2006,contrast 44 patients use somatostatin after pancreaticoduodenectomy as experimental group,and 56 patients with conventional therapy as control group.results:there were no pancreatic leakage case in the modified group of 44 cases.3 cases complicating pancreatic leakage occurd in control group,and the proportion of pancreatic leakage are different examined by statistic mathord.conclusions:use somatostatin after pd can restrain organism excrete diastase vera,and help anastomotic stoma to concrescent and decrease pancreatic leakage;pancreatic leakage should be treated by different grades,grade 1 can be cured by persistent drainage with somatostatin and nutritional support;grade 2 should be pay more energetic intervention.

作者单位：潍坊医学院，潍坊 261042；潍坊市人民医院肝胆外科，潍坊 261042 目的：探讨生长抑素在胰十二指肠切除术后减少胰漏的作用。方法：收集2003年1月～2006年12月施行pd患者100例，术后应用生长抑素持续泵入病人44例，并与同期施行的pd术后常规应用全肠外营养及抗生素治疗病人56例，结果进行了对比观察。结果：实验组病人44例无1例发生胰漏，对照组56例病人，3例发生不同程度的胰漏，发生率5.3%，统计学比较有显著差异（p＜0.05）。结论： pd后应用生长抑素持续泵入可以抑制胰酶的作用，使胰肠吻合口较好地愈合，从而有效地减少胰漏的发生；胰漏可进行分级治疗，ⅰ级胰漏采用通畅引流为主，同时使用生长抑素和营养支持等治疗多可治愈，ⅱ级胰漏需要积极治疗。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

Methods Twenty–four New Zealand white rabbits were divided into model group and control group randomly, of which the two groups which were made through the homemade impactive equipment by impacting the right acetabular cartilage of rabbit at 45cm with 0.400kg weight vertically and simulating the mechanism of impactive injury of human acetabular cartilage. Each group was 12.The model group was injected SH.The control group was injected NaCl solution. The observation of macrography, histology (stained by HE, safarnin O and toluidine blue), transmission electron microscope, scanning electron microscope and the method of TUNEL were made for the three groups that were killed postinjuriously at 4 day and 4 week.

方法新西兰大白兔24只，平均年龄6个月，体重2.5±0.5kg，模拟人髋臼软骨冲击伤机制，采用自制冲击装置，用质量为0.400kg的重锤，沿导向杆从高45cm处下滑垂直冲击兔右侧髋臼软骨，建立兔髋臼软骨冲击伤模型，随机分为模型组和对照组，每组12只。

The group disposed by Acetylspiramycin doesn"t show parallel results; The strip gray scale of exotoxin A decreased gradually with the increasing dosages of Azithromycin and Roxithromycin, which doesn"t show in group disposed by Acetylspiramycin and group PAO-JP2; The strip gray scale of lasR, detected by RT-PCR, decreased gradually with the increasing dosages of Azithromycin, which doesnt show in group PAO-JP2.Conclusions: There were obvious differences between PAO1 and PAO-JP2 in biology characteristics in vitro.

本研究发现大环内酯类药物在生物被膜培养过程中分别处理PAO1、PAO-JP2，结果提示大环内酯类药物(14、15元环)通过对QS系统的影响可有效抑制铜绿假单胞菌生物被膜的形成；通过QS系统有效抑制铜绿假单胞菌的毒力因子的产生，16元环的大环内酯类药物乙酰螺旋霉未见上述结论；lasR基因的反转录结果提示阿奇霉素可在转录水平抑制QS系统发挥其作用。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Among them, into the assets of the Group of Kyrgyzstan in order to transfer all the shares held by the company in the process of transfer from the Shan Coal Group to its name and to the trust after Hill Coal Group 7 of the furniture there is an independent legal personality of the coal trading company 100% equity; Hill for the injection of assets held by Coal Group 3 coal mining companies and 18 coal trading company with headquarters and its stake in the domestic coal business-related assets and liabilities of the underlying asset Hill Coal Group include coal mining and coal trading business two The integrity of the operating assets of the plate.

其中，进入吉尔吉斯斯坦集团的资产，以便将所有的该公司举行的移交给山煤业集团股票的过程和它的名字后山煤矿集团的7家具有一个信任独立法人资格的煤炭贸易公司100％股权，对因煤矿采煤集团三公司和总部和在国内煤炭企业的股份公司18日煤炭贸易有关的资产及相关资产负债的资产注入山山煤炭集团包括煤炭开采和煤炭贸易业务的两个板块的经营资产的完整性。

METHODS: Male Wistar rats were randomly divided into 4 groups: in sham group, only anesthetization and operation were performed; in MLR group, occlusion of mesenteric lymphatics for 1 h followed by 2 h of reperfusion; in SMAO group, occlusion of superior mesenteric artery for 1 h followed by 2 h reperfusion; in MLR+SMAO group, occlusion of SMA and ML for 1 h followed by 2 h of reperfusion.

Wistar雄性大鼠均分为4组：sham组，仅麻醉与手术；MLR组，夹闭肠系膜淋巴管1 h，再灌注2 h；SMAO组，夹闭肠系膜上动脉1 h，再灌注2 h；MLR+SMAO：夹闭ML和SMA1 h，再灌注2 h。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定，确认Line15是一个二体异附加系，附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关，并确定了Line15的外源染色体C-分带特征带型，为目后鉴定Line15附加系的真伪提供了简便快捷的途径；对抗旱附加系Line17进行了荧光原位杂交和SSR检测，得出Line17也是一个二体异附加系，外源染色体与小麦的第四同源群短臂密切相关。

Methods 40 healthy adult cats were equally devided into group A with sodiumchloride, group B with bFGF, group C with GbE and group D with bFGF+GbE in random. All animals were operated to establish the feline models of cerebral focal ischemia-reperfusion by the approach of left transorbital. The certain madicine intervene were take at 0 h, 24 h, 48 h after the models established. The neurological defects score was evaluated by Philips' criteria score in 1 d and 7 d. The physiological changes were observed by HE stained and electron microscope.

健康家猫40只，随机分为生理盐水对照组、bFGF治疗组、GbE治疗组和bFGF+GbE联合治疗组；采用左侧眼眶入路制作猫大脑中动脉缺血再灌注模型；制模后即刻、24h、48h分别给予相应的药物干预；制模后1d、7d分别进行神经功能缺损评分；制模后7d对脑组织切片进行光镜和电镜下病理及超微结构观察。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。