查询词典 group by group

Methods 37 patients was randomly divided into the test group(21 cases) and the control group(17 cases).The test group was treated by the method of iodine cautery combined with medicine,the control group was treated only by medicine.The therapeutic effect between the two groups were analysed by statistics analysis.

37例真菌性角膜溃疡的患者随机分为治疗组21例和对照组16例，治疗组应用碘酊烧灼联合药物治疗，对照组单纯应用药物治疗，对两组疗效进行统计学分析。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

Results: Enter 38 patients of clinical pathway group, press the surgical operation method to is divided into abdominal panhysterectomy group and panhysterectomy by abdominoscope group;The non- clinical pathway group chooses to use the last year same period patient 34, press the surgical operation method to is divided into abdominal panhysterectomy group and panhysterectomy by abdominoscope group.

结果：进入临床路径组病人38例，按手术方式分为腹式手术组和腹腔镜手术组；非路径组34例，按手术方式分为腹式手术组和腹腔镜手术组。

Objective To observe the pathological changes and investigate the hepatocyte apoptosis relating with CYP2E1 in alcoholic liver diseaseof rat. Method the Model of Alcoholic liver Disease: Rats were randomly divided into model group(n=37)and control group(n=33). The dose of 40％ethanol was administerd at 8g/kg body weight by garage twice daily for 8 weeks in model group, and control group was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detect by autobiochemistry instrument. The pathological changes of liver was observed under light microscope after HE staining, hepatocyte apoptosis was detected by the TUNEL method.

目的： 研究酒精性肝病细胞凋亡程度与细胞色素P4502E1表达的关系方法：用酒精灌胃法制备大鼠酒精性肝病模型，将70只wistar大鼠随机分为正常对照组(33只)和酒精灌胃组(37只)，连续灌胃8周后病理切片观察细胞凋亡程度，干化学法测定ALT、AST的含量变化，用PCR法测定细胞色素P450 2E1的表达变化。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Results: 1. The value of all experimental indexes decreased significantly after heat stress, and reached the lowest at the time 2-8 hours after heat stress. This indicates that the immunity of the mice is damaged to the largest extent at 2-8 hours after heat stress. 2. L-arginine supplementation with appropriate dose could remit the acute atrophying of thymus and spleen tissue caused by heat stress. 3. After L-arginine supplementation with appropriate dose, the lymphocyte proliferation, the level of concentration of IL-2 and * expression of IL-2R raised in the group with room temperature; in the heat stress group the level of three indexs decrease significantly. Furthermore, the decrease of the group given 1 .Smglg.bw L-arginine is the smallest. This result indicates that the concentration of 1 .Smglg.bw L-arginine supplementation could remit the depressment of the immunity caused by heat stress. 4. The [Ca2~] in activated thymocytes of the group with L-arginine -2- supplementation is significantly higher than that of the group with water supplementation. This indicates that L-arginine supplementation could protect thymoeytes of mouse under heat stress. We also found that the fluorescence intensity of [Ca2~] in activated thymocytes of the group given 1 .Smglg.bw L-arginine is the highest.

结果：1、热应激后小鼠的各项免疫指标均有显著性下降，其中在2.8小时降到最低，提示在这个时间段小鼠的免疫功能受到最大损害；2、适量补充精氨酸有助于缓解热应激导致小鼠胸腺和脾脏的急性萎缩；3、常温组，补充适量精氨酸后小鼠淋巴细胞增殖活性、IL-2的浓度及IL-2R的表达均有显著性上升，而热应激后三指标均显著降低，其中在精氨酸给予浓度为1.5mg/g.bw时三指标的降低程度最少，提示该浓度可最大程度缓解热应激对小鼠免疫功能的抑制；4、补充精氨酸组的活化胸腺细胞［Ca~(2+)］较给水组有显著性上升，说明精氨酸对热应激小鼠胸腺细胞具有保护作用，并发现在精氨酸给予浓度为1.5mg/g.bw时活化胸腺细胞的胞浆钙离子荧光强度达到最大；5、热应激后小鼠血清NO的浓度均有不同程度的上升，补充精氨酸后血清NO上升更加明显，但并非随精氨酸给予浓度的增加而增加。

Basing on the setup of animal model of root avulsion of the brachial plexus, we divided the experimental animal into four groups by the operative method: Group A: one end of the maternal nerve graft was connected with the upper trunk of the healthy side by end-to-side neurorrhaphy, and the other end was sutured to the distal stump of musculocutaneous nerve infraclevicularly by end-to-end neurorrhaphy. Goup B: on the healthy side, the operation was the same, the other end was sutured to the distal end of C〓 by end-to-end neurorrhaphy on the affected side. Group C: the maternal nerve graft bridged the phrenic nerve and infraclevicular musculocutaneous nerve of the affected side by end-to-end neurorrhaphy. Group D: the phrenic nerve was sutured to the distal end of C〓 supraclevicularly by end-to-end neurorrhaphy.

在构建了模拟小儿臂丛神经根性撕脱伤的动物模型基础上，将实验动物按手术方式分组如下，A组：母鼠提供的的神经移植体一端与子鼠健侧臂丛上干行端侧吻合，另一端与患侧已切断的锁骨下肌皮神经远端行端端吻合。B组：母鼠提供的的神经移植体一端与子鼠健侧臂丛上干行端侧吻合，另一端与患侧已切断的锁骨上颈〓远端行端端吻合。C组：母鼠提供的的神经移植体桥接于子鼠患侧膈神经与锁骨下肌皮神经之间。D组：子鼠患侧膈神经直接与锁骨上颈〓远端行端端吻合。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

METHODS Thirty six Wistar female rats used in experiment were randomly divided into three groups: the normal control group, 45 d group and 90 d group with the revival liquid of quantum. All rats were administered by free drinking, the normal control group were treated by drinking water in 3 months, 45 d group was performed drinking water in 1.5 months and then revival liquid of quantum in 1.5 months, and the 90 d group was administrated the revival liquid of quantum in 3 months.

方法］ 取雌性Wistar大鼠36只，按体重随机分为对照组、量子复活液45 d组和90 d组；以自由饮用方式给药，给予对照组大鼠普通自来水喂养90 d，给予量子复活液45 d组大鼠普通自来水45 d后，再给予量子复活液喂养45 d，给予量子复活液90 d组大鼠量子复活液90 d。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。