查询词典 greenhouse effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The system through the temperature examination electric circuit sensation greenhouse big awning temperature change, gives monolithic integrated circuit processing through a/d conversion, through keyboard hypothesis upper limit temperature and lower limit temperature, when the greenhouse big awning temperature is lower than the hypothesis the temperature lower limiting value, start heater unit to greenhouse warming, when the greenhouse big awning temperature is higher than the hypothesis in the temperature the limiting value, stopped heating up, has realized the greenhouse big awning observation automation.

系统通过温度检测电路感知温室大棚温度变化，通过模数转换送给单片机处理，通过键盘设定上限温度和下限温度，当温室大棚温度低于设定的温度下限值时，启动点暖风机给温室加温，当温室大棚温度高于设定的温度上限值时，停止加热，实现了温室大棚测控的自动化。

Velocity field inside of arciform greenhouse , fastigium greenhouse , parabola greenhouse and slanting crest greenhouse have been studied by numerical simulation of 3D turbulent flow.

本文对拱型、屋脊型、抛物型、斜顶型大棚这四种断面型式的大棚采用上述紊流模型，对其内部流场的流动进行了数值模拟。

Learning by doing in truth achieves the abatement of greenhouse gases; it gives a hint government has to promote the action for abatement of greenhouse gases; hence, we can approach to the decoupling circumstance for greenhouse gases emission advanced.

做中学的确有助於温室气体减量成效的提升，隐含政府应及早推动温室气体减量行动，可以及早达到温室气体排放绝对脱钩之结果。

The resultsshow: the time of exchanging gas of the slanting crest is the most shortest, the second is the parabola greenhouse, the third is the arciform greenhouse ,the fourth is the fastigium greenhouse.

计算结果表明：斜顶型大棚换气时间最短，抛物型大棚次之，拱形大棚再次之，屋脊型大棚换气时间最长。

The main environment factors which affect the growth of plants are temperature, humidity, and sunshine etc, but it iswarm at most part of China East area in our country, the warming time is not long in winter, the greenhouse temperature is high in summer and the highest room temperature is often upper than 40℃, so 2/3 of the year we can not plant in greenhouse, which dilapidates the greenhouse over long time.

影响植物生长发育的主要环境参数有温度、湿度、光照等，但是我国华东大部分地区气候温暖，冬季温室加温时间不长，夏季温室室温高，最高室温常在40℃以上，植物无法生长，一年有三分之二时间不能栽培作物，造成温室长期间荒废。

This article describes the process of further changing the sunlight greenhouse into the multi greenhouse, of which the inside is crossed, double-pointed and narrow, and installing the greenhouse with this special structure to the wall below the outside of the window of building to form a window -type solar thermal utilization device.

文章介绍了将日光温室进一步改造为内部成交叉双尖窄状的多重温室，并将这种特殊结构的温室安装在建筑物窗户外面下端的墙壁上，就构成一种窗户型太阳能热利用装置。

This equipment uses advanced micro-computer technology, digital sensor technology and automatic control technology, mainly used in agriculture and greenhouse vegetables, greenhouse nursery, greenhouse flowers, Hanabusa, plant nurseries, vegetable preservation library, poultry farming, animal husbandry, as well as warehouses and grain storage and other places.

本仪器采用了世界上先进的微电脑技术、数字传感器技术以及自动控制技术，主要应用在农业蔬菜大棚、育苗温室、花卉温室、花房、苗圃、蔬菜保鲜库、家禽养殖、畜牧养殖以及仓库、粮库等场所。

The soil environment of solar greenhouse deteriorate with the growing ages increased and this causes the obstacle matters in soil of succession planting cucumber, mainly perform as soil physicochemical properties worsening, soil hypo-salinization, soil acidification. To solve these problems, corn and spring onion as catch crops were planted in summer after cucumber harvest that based on having no effect on cucumber production in solar greenhouse.. The experiment researched the effect of different catch crops on cucumber yield and soil physicochemical properties.

针对日光温室黄瓜生产中存在土壤理化性状劣化、土壤板结、盐渍化、酸化等土壤退化问题，在保证黄瓜产量的前提下，夏季休闲期选择玉米、大葱作为填闲作物，以农民传统的常规休闲作为参比对象，研究玉米、大葱对保护地土壤理化性状以及对黄瓜产量的影响。

The effect of treatment is negatively correlated with the course of disease, generally, the effect is much worse with the course of disease is much longer; The effect is positively correlated with the course of treatment, it is better if the course of treatment is longer.The group of more than one year is much excellent than the group of less one year. The ex-electroencephalogram before treatment is normal, on edge and a little abnormal, the effect is better. As regards CT or MR result, there is difference between the effective group and the ineffective group. The result of CT and MR is negative, the effect is better, and the effect to demyelination and leukoencephalopathy is most worst.However,for the tipe of much abnormality, the effect is the most worst.The effect is related to course of disease.The effect is better if the course is shorter.There is no difference in the tipe of disease the symptoms before episode, the frequency of episode, the inducing reasons, the age, sex and so on.

病程的长短与疗效存在负相关关系，病程越长疗效越差；疗程与疗效存在正相关关系，疗程越长疗效越好；疗前脑电图的异常程度与疗效存在负相关关系，正常、边缘及轻度异常者，疗效较好，重度异常者疗效最差；无效组与有效组在CT及MR检查结果上有差异，有阴性者疗效好，阳性者疗效差，脱髓鞘及脑白质病疗效最差的趋势；疗效与性别无相关性；无效组与有效组在发作类型、既往病史、辨证分型、发作先兆、疗前发作频率、诱因、发病年龄、就诊前所用药物方面无差异。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？