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Dhar AK, Roux MM, Klimpel KR. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR Green chemistry[J].

研究过程发现SYBR Green I实时荧光定量RT-PCR与巢式RT-PCR相比,具有线行关系好、线性范围宽等特点;并且,扩增和检测在同一管内完成,不需要开盖,不需要电泳检测PCR产物,有效解决了污染问题[10]。

Methods Both immatured and matured oocytes were stained by Mito Tracker Green FM.

方法未成熟卵母细胞和成熟卵母细胞均用Mito Tracker Green FM染色,经多聚甲醛固定后在激光扫描共焦显微镜下观察线粒体的分布。

"Davidia involucrate" was a rare valuable and endangered plant in China. In this article, We studied the distribution pattern of its population with t test of Dispersal index, X^2 test of Poisson distribution and F test of Morisita's index. Its assemble intensity is also analyzed through Negative binomial index, Green's index, Lloyd's index and Patchness index.

采用扩散系数的t检验、Poisson分布的x^2拟合检验及Morisita指数的F检验来研究珙桐种群的空间分布格局类型,用负二项参数、Green指数和Lloyd的平均拥挤度m(上标*与聚块性指标来研究珙桐的聚集强度。

And the sherbet melts in our hands and leaves stains of lemony yellow, melody orange and sour green.

留下柠檬黄色,橙色和酸绿色的污点(lemony yellow, melony orange, sour green)。

According to the Green's approach in theory of mass load on Earth's surface, the vertical displacements at some Chinese IGS stations due to mass load, including atmosphere, nontidal ocean and land water, are estimated.

根据地表质量负荷理论的Green函数方法,计算了地球表面质量负荷(包括大气、非潮汐海洋和陆地水)对中国部分IGS台站垂直位移的影响。

Green Mountain, the highest summit, becomes Cadillac Mountain, Newport becomes Champlain Mountain, Robinson becomes Acadia Mountain, Brown's becomes Norumbega Mountain, and Dry becomes Fly-ing Squadron Mountain.

Green Mountain这一最高峰已改成Cadillac Mountain,Newport则变成了Champlain Mountain,Robinson变成了Acadia Mountain, Brown's 变成了Norumbega Mountain,而Dry变成了Flying Squadron Mountain。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的:构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha,HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein,hrGFP)报告分子的新型腺病毒真核细胞表达载体。

To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn, TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1,建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系,并对体系进行优化。

This dissertation makes horizontal well and symmetric multibranch horizontal wells as research object, according to reservoir characteristics and the seepage process of horizontal well, using multidisciplinary knowledges comprehensively including reservoir geology, reservoir seepage theory, point source function, Green function, conformal transformation, partial differential equation and computational mathematics et al, then researches theory and method suitable for describing seepage process and productivlty calculation of horizontal well.

本文以水平井和对称多分支水平井为研究对象,从油气藏的储层特征和水平井的渗流过程出发,综合运用油气藏地质、油气藏渗流理论、点源函数、Green函数、保角变换、偏微分方程及计算数学等多学科知识,研究出适合于描述水平井渗流与产能计算的理论与方法,主要取得了以下成果

According to the method for the bending analysis of composite laminates developed by the authors, Green's functions of an infinite unsymmetric laminate with elliptic hole or crack are obtained.

运用作者建立的复合材料层合板问题的求解方法,得到了含椭圆孔和裂纹的无限大非对称层合板在广义集中载荷作用下的Green函数。

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推荐网络例句

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。