查询词典 graphic expression

Therefore, NAG7 gene was a nice candidate of tumor suppresser gene associated with NPC. The mechanism might be that the re-expression of NAG7 gene contributed to the change of gene expression profile and following the change of protein expression profile, then involved in the cell cycle and apoptotic regulation changed, and finally suppressed the NPC growth.

因此，NAG7基因作为鼻咽癌相关的抑瘤基因候选者，其作用机理可能是通过诱发基因表达谱的改变而引起蛋白质表达谱发生相应的改变，在一致性改变的功能分子的作用下，抑制细胞增殖，同时诱发细胞凋亡增加，从而发挥抑制鼻咽癌细胞生长的作用。

The results of SDS-PAGE showed that the expression products were about 31KD and reached their expression peaks about 2 hours after inducing and the expression quatity of fusion protein had 22.7% ratio of total thalline protein, while the E.

提取并纯化pET-PIL-8的融合表达蛋白并用改进的琼脂扩散法检测其生物学活性，通过检验分析表明所获得的重组猪IL-8具有趋化猪外周血淋巴细胞的作用，具有较强的生物学活性。

After amputation, the expression of desmin appears first in the distal end epidermis and the peritoneum 4 d PA. The expression in the peritoneum lasts to the 14 d PA when expression of desmin appears in the musculature, while that in the distal end epidermis only lasts to the 8 d PA when the connective tissue below begins to express desmin. Moreover, from the 4 d PA, musculature and epidermis of the tube foot begin to express desmin and the expressions last to the 20 d PA. From 8-14 d PA, musculature of the ambulacral plate expresses myogenin and the peritoneum also expresses myogenin from 14-20 d PA. All these results indicate that precursor cells for muscle regeneration in adult starfish A.rollestoni are mainly from the distal end epidermis and the parietal peritoneum.

创伤后第4 d，顶端表皮层及体腔上皮中首先出现了desmin表达，体腔上皮中desmin的表达一直持续到第14 d，此时其上方的肌肉组织中也出现了desmin表达；而顶端表皮层中desmin的表达仅持续到第8 d，同时其下方的结缔组织也开始表达desmin；此外，从第4 d起，管足肌肉及管足表皮层开始表达myogenin，且该表达一直持续到创伤后的第20 d；而第8 d起，步带板肌肉也出现了myogenin的表达，此表达一直持续到第14 d；从第14 d起，体腔上皮也出现myogenin表达，且一直持续到第20 d。

Include: Of history of civilization of baleful and misinterpret China, China and historical historical fact; Baleful and misinterpret his country the history, do not respect what civilization and custom are used to human civilization, his country; Derogate in cold blood, evil of figure of the anybody in doing famous book of character of revolutionary cacique, hero, important history character, China and foreign countries and famous work; Ill will derogates people army, armed police, public security and judicatory figure; Have mistreat captive, inquisition by torture to extort a confession of the clue such as convict or guilty suspect; Expression violates guilty aggressive arrogance, specific reveal criminality detail, reveal special investigation shift, expose what ought to be protected to inform against the figure such as person, witness, audio; Advertise religious ultraism, instigate between each religion, denomination, the contradiction between be religious and masses of lack of faith and conflict, harm masses emotive; Advocate sees photograph, fortune-telling, see ghost of geomantic, augur, drive treat a disease those who wait for feudal superstition activity; With evil do means to depict heavy nature calamity, contingency, horrible incident, war to wait for catastrophic occasion; Specific show licentious, rape, incestuous, love cadaver, walk the street, go whoring, of the clue such as sexual allergy, masturbation; Of the detail such as means of process of expression or obscure performance sexual behavior, sex, sex and as associated as this overmuch body contact; Show intentionally, be masked with limbs only or cover position of human body privacy with very small pall; Contain sexual suggestion, sex to tease those who wait to make the person produces a gender to associate easily; Advocate extramarital love, much horn is loved, one-night standing, sex is mistreated and change wife wait for insalubrious content; In order to grow up the film, affection lubricious film, 3 class piece, pat secretly, expose one's body, dew point reachs all sorts of flirtation to sexual written language or picture regard video program as caption or be classified; Have intense of the clue such as excitant homicide, bloody, violent, suicide, kidnap, drug taking, gamble, clever different; Have picture of excessive fright bloodcurdling, caption, background music and sound effect; Specific show animal of cause sb's death by maltreating him, catch kill, edible country protects kind of animal; Contain those who encroach individual privacy content; With affirmative, favorable fundamental key or fetching me-too means expression fights other people of affray, shame, corrupt of character bawdry; The philosophy with inactive, decadent advocate, world outlook and viewpoint of value, unwisdom of sedulous apply colours to a drawing, exaggerated nation lags behind or of social the seamy side; The cutout of the program of movie and TV that total bureau of TV of national broadcast film prohibits travelling and film, teleplay reduces part; Violate relevant law, statute of spirit.

包括：恶意曲解中华文明、中国历史和历史史实的；恶意曲解他国历史，不尊重人类文明、他国文明和风俗习惯的；蓄意贬损、恶搞革命领袖、英雄人物、重要历史人物、中外名著及名著中重要人物形象的；恶意贬损人民军队、武装警察、公安和司法形象的；有虐待俘虏、刑讯逼供罪犯或犯罪嫌疑人等情节的；表现违法犯罪嚣张气焰，具体展示犯罪行为细节，暴露特殊侦查手段，暴露应当受到保护的举报人、证人等形象、声音的；鼓吹宗教极端主义，挑起各宗教、教派之间，信教与不信教群众之间的矛盾和冲突，伤害群众感情的；宣扬看相、算命、看风水、占卜、驱鬼治病等封建迷信活动的；以恶搞方式描绘重大自然灾害、意外事故、恐怖事件、战争等灾难场面的；具体展现淫乱、强奸、乱伦、恋尸、卖淫、嫖娼、性变态、自慰等情节的；表现或隐晦表现性行为、性过程、性方式及与此关联的过多肉体接触等细节的；故意展现、仅用肢体掩盖或用很小的遮盖物掩盖人体隐私部位的；带有性暗示、性挑逗等易使人产生性联想的；宣扬婚外恋、多角恋、一夜情、性虐待和换妻等不健康内容的；以成人电影、情色电影、三级片、偷拍、走光、露点及各种挑逗性文字或图片作为视频节目标题或分类的；有强烈刺激性的凶杀、血腥、暴力、自杀、绑架、吸毒、赌博、灵异等情节的；有过度惊吓恐怖的画面、字幕、背景音乐及声音效果的；具体展示虐杀动物，捕杀、食用国家保护类动物的；带有侵犯个人隐私内容的；以肯定、赞许的基调或引人模仿的方式表现打架斗殴、羞辱他人、污言秽语的；宣扬消极、颓废的人生观、世界观和价值观，刻意渲染、夸大民族愚昧落后或社会阴暗面的；国家广播电影电视总局禁止传播的影视节目以及电影、电视剧的删减片段；违反相关法律、法规精神的。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

To explore the possibility that the androgen may facilitate differentiation of hair follicle stem cells into sebocytes, we carried out experiments to test this hypothesis. The concrete steps to carry out this research were as follow: Firstly, observed the expression of AR and examined the expression of AR mRNA of rat vibrissa follicle stem cells in vivo in three defined phases of a hair follicle cycle, including anagen, catagen and telogen. Secondly, cultured HFSC deprived from vibrissa follicle bulge region in culture flasks and observed the expression of AR, ketertin 15 (K15), cluster of differentiation 34 (CD34) andβ1-integrin of those cells.

为了探索雄激素对毛囊干细胞定向分化为皮脂腺细胞的过程是否具有促进功能，我们进行了如下的实验来进行研究：第一步，检测了在毛囊周期的三个不同时期(生长期、退化期和静止期)中大鼠触须毛囊干细胞中AR、ARmRNA的表达以及检测原代培养的毛囊干细胞中AR的表达并探讨意义，为进一步研究打下基础。

Results With the expression in normal salivary gland as a standard, low C-erbB-2 mRNA expression was seen in adenolymphoma, basal cell adenoma. However, various degrees of over-expression of C-erbB-2 oncogene mRNA were detected in pleomorphic adenoma, mucoepidermoid carcinoma,acinic cell carcinoma ,adenoid cystic carcinoma ,papillary cystic carcinoma,and myoethelial cell carcinoma.

结果 正常涎腺和涎腺肿瘤中发现不同程度C-erbB-2mRNA的表达，以正常涎腺组织的表达水平为基准，在腺淋巴瘤、基底细胞腺瘤呈现低表达、无表达或与正常涎腺组织表达水平相似，多形性腺瘤、粘液表皮样癌、腺泡细胞癌、腺样囊性癌、乳头状囊腺癌和肌上皮癌则呈现不同程度的过表达。

Here we summarize the progress of salmon calcitonin's heterogenous expression in prokaryotic and eukaryotic expression systems as well as the amidation of C-terminal. Both the biological activity of salmon calcitonin and the biologic expression system can be improved by genetic engineering techniques.

本文综述了鲑鱼降钙素基因在大肠杆菌、链霉菌、乳酸杆菌和蓝藻等原核细胞，在酵母、昆虫、动植物等真核细胞中异源表达的研究进展，以及通过基因工程的方法能够改进生物表达系统，提高鲑鱼降钙素活性的动态。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results The small regenerating fibers, central location of nucelus and basophilia endochylema were observed under HE stain both in the patients with DMD and PM. Anachromasis of antiNCAM monoclonal immunohistochemistry stain was found in the regenerating muscle fibers. Positive Ezrin expression was also detected in the regenerating fibers. However, this expression tapered gradually as the mature process of skeletal muscle. The expression of Ezrin was negative in the mature fibers.

结果 DMD、PM患者被检肌HE染色所见再生肌纤维直径较小、核位于中央、胞浆嗜碱性；NCAM染色再生肌纤维深染；再生肌纤维Ezrin呈阳性表达，伴随肌纤维成熟Ezrin表达逐渐减弱，成熟肌纤维无Ezrin表达；成肌细胞Ezrin呈阳性表达。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。