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The mRNA expression of MMP-2 in more than 4 lymphnodes positive is lower than in lymphnode 1-3 positive （P＜0.05）.The MMP-2 mRNA expression is irrelated with tissue grade,pathologic type,ER,PR and Her-2.Conclusion MMP-2 mRNA expression is upregulated in early clinical stage,lymph node metastatic early stage,and down regulated with tumor progressing.MMP-2 mRNA expression is related with the metastasis of breast cancer.

MMP-2 mRNA转录水平的表达与乳腺癌转移有关，MMP-2在转录水平的表达在肿瘤较小时上调，随着肿瘤的增其转录水平表达下调，随着肿瘤恶性程度的增加，其转录水平的表达量在下降，在肿瘤发生的早期表达上调，随着肿瘤的进展表达量下调。

WTHZ Results The telomerase activity and expression of telomerase rela ted genes were significantly higher in 42 leukemia patients in progress stage th an that in complete remission/chronic stage and in normal/anemia cases (P.01).The telomerase activity and expression of TP1,hTR gene in 14 cases of acute leuk emia complete remission/chronic myelogenous-chronic stage were h igher than that in normal/anemia cases (P.05). Low levels of telom erase activi ty and telomerase related genes expression could be detected in part of normal b one marrow cells. The levels of telomerase activity and telomerase related genes expression in 3 cases of anemia increased after short-term culture.

结果 42例白血病进展阶段标本的端粒酶活性和相关基因表达水平高于急性白血病完全缓解期、慢性粒细胞白血病慢性期、正常和贫血标本（P.01）；14例急性白血病完全缓解期和慢粒慢性期标本端粒酶活性和TP1、hTR基因表达水平高于正常和贫血标本（P.05）；在部分正常和贫血标本中检测到相对低水平的端粒酶活性和相关基因表达，3例贫血标本经短期常规培养后检测到端粒酶活性和基因表达增高。

The higher VEGF-C expression and LVD expression in esophageal cancer, the higher risk of lymph node metastasis in esophageal cancer; podoplanin marked LVD is more precise than VEGFR-3 marked LVD, and expression of podoplanin correlates closely with high expression of VEGF-C and LVD. It is a satisfactory lymphangial endothelium marker.

癌旁组织LVD同VEGF-C的高表达及淋巴结转移密切相关，食管癌组织中podoplanin标记的淋巴管密度较VEGFR-3标记的淋巴管密度更为精确，是一种更为满意的淋巴管内皮特异性标志物。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

Results 1. The expression of P53, KI-67, hTERT and ToPo II a protein was higher in astrocytoma than that in nomal brain tissue, the expression of these markers was obversely correlated to the grade of astrocytoma (P.05). The expression of Fas was higher in astrocytoma than that in nomal brain tissue, on the contrary, expression of P16 was lower in astrocytoma than that in nomal brain tissue.

结果 1、星形细胞瘤中P53、KI-67、hTERT与ToPoⅡα蛋白表达阳性率高于正常脑组织，P53、KI-67、hTERT与ToPoⅡα蛋白表达与星形细胞瘤的病理组织学分级呈显著正相关(P.05)；Fas蛋白在星形细胞瘤中的表达高于正常脑组织，而P16蛋白表达低于正常脑组织，Fas、P16蛋白表达与组织学分级无显著相关性。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.

研究结论：(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化；(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠，使外源AT2R基因的表达处于有效的主动控制下；(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生；(4)由MSC介导的AT2R可调控表达受到Dox的良好调控，可有效抑制球囊损伤后大鼠颈动脉新生内膜增生；(5)AT2R影响血管损伤后修复时ECM成分的表达，并促进细胞凋亡的发生，可能是AT2R介导抑制新生内膜形成的机制。

Deduced by the proportion of typical sterile plants and normal fertile plants in segregative generations derived from WA zhenqiuA/6078 that:I gene could inhibit the expression of Rf gene completely by the heterozygosity of 1 pair of Rf in genotype;and only reduce the expression of Rf genes with two heterozygous Rf genes in genotype ;but it would never inhibit the expression of Rf genes if the genotype included 3 pairs of heterozygous Rf genes. When Rf gene was homozygous,the / gene could not inhibit the expression of it.

根据认叭真秋刀6078各衍生分离世代中典型不育株和正常可育株所占比例推断：基因型中仅包含1对杂合的Rf基因时，I对Rf的表达起完全抑制作用；基因型中包含2对杂合的Rf基因时，I仅对Rf的表达起削弱作用；当基因型中包含3对杂合的对基因时，I对Rf的表达不起抑制作用；I对纯合位点的Rf的表达不起抑制作用。

Estrogen deficiency can increase the mRNA expression of MMP-1、MMP-3 and IL-1β in OA synovia tissue. Estrogen could inhibit the mRNA expression of MMP-1、 MMP-3 and IL-1β in OA synovia tissue. Progestin could even decrease this expression. Estrogen could slightly increase the mRNA expression of TIMP-1. It is suggested that low dose of estrogen supplement play a protective effect on OA cartilage, and progestin could increase this protective effect.

雌激素缺乏可使OA关节滑膜中MMP-1、MMP-3和IL-1β mRNA表达增加，雌激素对关节滑膜组织中MMP-1、MMP-3和IL-1β mRNA表达有抑制作用，孕激素可增强雌激素的这一抑制作用，雌激素对TIMP-1有轻度升高作用，提示低剂量雌激素对OA关节软骨有一定保护作用，而加用孕激素这一保护作用增强。

A parenthesized expression list yields whatever that expression list yields: if the list contains at least one comma, it yields a tuple; otherwise, it yields the single expression that makes up the expression list.

括号内表达式列表的结果取决于其内部表达式列表的结果：如果表达式列表中包括至少一个逗号，它就生成一个元组；否则，就生成一个由表达式列表组成的表达式。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。