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SCAP easy occurred in the aged people. The pathogen of sputum included 76.0% of gram- negative bacteria and 24.0% of gram- positive bacteria.

1.3.3 细菌分离和鉴定：合格的痰标本接种在血平板上，35℃培养后，按常规方法分离，并采用法国生物梅里埃公司VITEK- AMS鉴定。

The invention forms a new soil layer structure with the upper part rough and the lower part fine, increases a coarse sand content of upper soil and obviously improves soil tilth; thereby facilitating the leaching of the salt. Only half a year is needed and then the salt content of a plow layer is reduced from six point five four gram per kilogram to one point two three gram per kilogram; thereby speeding up the improvement and utilization speed of the marine solonchak and restraining the uprise of salt.

本发明形成上粗下细的新土层结构，增加了上层土壤的粗砂粒含量，明显改善了土壤耕性；有利于盐分的淋洗，仅需半年时间，耕作层含盐量即可由6.54g/kg降至1.23g/kg，加快了滨海盐土的改良利用速度，并抑制了返盐现象。

Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity.

为建立一种革兰氏阴性及阳性菌均能分析的质粒提取方法，以Biswajit saha的方法为基础，对比酸酚法、热变性法、碱变性法、酶法等方法，研制符合国内一般实验室的方法。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体，采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻，通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测，同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性，结果表明NP-1基因已稳定整合到小球藻基因组中，并进行了正确转录和表达。

One tablespoon has 27 calories, 1 gram of fat and 1 gram of fiber.

一勺中含有27卡热量，1克脂肪和1可纤维。

Hunan also has one kind of peasant family self-restraint hot pepper sauce, the manufacture method: 2 kilogram small red pepper cleaning, chops Cheng Mizhuang, makes into the thick liquid with the whipper pappy, adds 50 gram garlic rice, 50 gram Jiang Mi, 40 grams salts, 25 grams liquor to mix evenly highly, places native manufacture Tao Tannei, with the clear plastic wrap seal, covers the top head, adds the world along the water, about one week then edible, may lay aside 1-2 years in the room temperature shady place not to deteriorate.

湖南还有一种农家自制辣椒酱，制作方法：把2千克小红椒洗净，剁成米状，用搅打器打成浆糊状，加50克大蒜米、50克姜米、40克盐、25克高度酒二锅头）拌匀，放在土制陶坛内，用保鲜膜封口，盖上盖，加坛沿水，一个周左右即可食用，可在室温背阴处放置1－2年不变质。

Afterwards, theater investor Lowell (faction bent gram Willson to play the part of) recognizes the gram Li silk 汀 is his childhood plays the partner, two people fell deeply in love.

后来，戏院的投资人劳尔认出克莉丝汀是他的儿时玩伴，两人坠入爱河。

Of them were Gram-negative rod, 3.8% were Gram-positive. The dominant genera were Vibrio(28 strains). The genera were also composed of Flavobocterium(6), Pseudomonas(5), Achromobacter(5), Aeromonas(3), Enterobacteriaceae(2), Bacillus(2). 1 strain isolated from the oyster died in reproduction.

优势菌为弧菌属28株，其次是黄杆菌属6株、假单胞菌属5株、无色菌属5株、气单胞菌属3株，此外还有肠杆菌科2株、杆状菌属2株，另有1株细菌在传代中失去了生长能力。

Fifty-four strains of pathogens were isolated from 28 cases with VAP, the percentages of Gram-negative bacilli, Gram-positive cocci and fungi were 83.33%, 7.41% and 9.26% respectively. Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Achromobacter xylosoxidans were the most common pathogens.

共检出病原菌54株，其中革兰阴性菌45株，占83.33%，前三位致病菌依次为铜绿假单胞菌、嗜麦芽窄食单胞菌和木糖氧化产碱杆菌；革兰阳性菌4株，占7.41%；真菌5株，占9.26%。

Acne repaired mask: 10 gram Nacre Stratum Powder, mixed with 5 gram adlay powder, right amount of mashed balsam pear and mashed cucumber. Apply on face and leave for 15-20 minutes, then rinse off.

祛痘修复面膜：约10克珍珠层粉与约5克薏仁粉、适量苦瓜泥或黄瓜泥、调匀涂抹于脸上，保留15 - 20分钟，然后洗去。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。