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There was 43 species of pathogenic bacterium and 356 stocks in all, 125 gram-positive bacteria, 221 gram-negative bacteria and 10 eumycete.

分离出病原菌43种356株，其中革兰氏阳性菌125株，占35.1%；革兰氏阴性菌221株，占62.1%；真菌10株，占2.8%。

The proportion of Gram-negative bacilli,Gram-positive cocci and eumycete was 68.0%,23.4% and 8.7% respectively in 184 isolated strains.

铜绿假单胞菌（18.5%）、嗜麦芽窄食单胞菌（14.1%）、洋葱伯克霍尔德菌（10.9%）、金黄色葡萄球菌（10.3%）和鲍曼不动杆菌（8.7%）为VAP最常见的病原菌。

A bacteriocin-like substance producing strain T12 was screened from 212 lactic acid bacteria isolated from intestine of healthy pigs by paper disk test and was identified as Lactobacillus. The supernatant of T12 exhibited strong inhibitory activity against the gram-positive bacteria, including Micrococcus lysodeikticus, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. A strong activity was also observed against the gram–negative bacteria and some eumycete, such as Escherichia coli, Salmonella typhimurium and Fusarium graminearum.

通过滤纸片法从健康肉猪猪大肠、小肠中分离得到212株抗生物质产生菌，以杯碟法复筛，得到1株对溶壁微球菌、金黄色葡萄球菌、枯草芽孢杆菌、蜡状芽孢杆菌等革兰氏阳性菌和大肠杆菌、鼠伤寒沙门氏菌等革兰氏阴性菌以及部分真菌如禾谷镰刀霉均有强烈抑制作用的乳酸菌。

The main pathogens of VAP were Gram negative organisms (68.63%) whose predominant pathogens were Pseudomonas aeruginosa and Acinetobacter. On the other hand, 75.40% of Gram positive organisms were Staphylococcus epidermidis.Of the pathogens 20.98% were eumycetes. Compared from June 1996 to June 2001 with from July 2001 to June 2006,there was no significant difference on the incidence rate of P.aeruginosa(31.76% vs 31.58%).During the recent 10 years,the incidence of Acinetobacter rose up from 8.23% to 15.23% and the rate of oxacillin-resistant Staphylococcus epidermidis rose up from 18.75% to 71.43%. The sensitivity of P.aeruginosa and Acinetobacter isolates descended to sodium imipenem cilastatin and ceftazidime in past 10 years.

在538例VAP中，2种及2种以上致病菌感染占56.51%；革兰阴性细菌中，铜绿假单胞菌和不动杆菌属为VAP的主要致病菌(68.63%)(385/561)；表皮葡萄球菌感染为VAP的主要革兰阳性致病菌(75.40%)(95/126)；真菌占20.98%(183/872)；铜绿假单胞菌在两个时间段所占比例无明显变化，不动杆菌属的检出率呈上升趋势(8.23%～15.23%)；铜绿假单胞菌和不动杆菌属对亚胺培南、头孢他啶的敏感性明显下降，耐苯唑西林表皮葡萄球菌的检出率显著升高(18.75%～71.43%)。

Increasing with the degree of inflammation, the proportion of gram positive facultatively anaerobic oral Streptococcus goes up and gram negative anaerobic rods goes down.

炎症的产生可能是由于多种混合菌的感染，导致机体和口腔常居细菌之间平衡关系被打破而造成的一种生态失调性疾病。

The result indicates that every 20 gram Lithospermum erythrorhizon oil can eventually produce 10 2 gram methyl esters of fatty acids and the weight perce...

实验结果表明：每 2 0g紫草油最终可得不饱和脂肪酸甲酯 10 2g ，其中w =83 1%。

The central theme of this thesis is to investigate the expression of lymphocyte surface marker and concentration of serum cytokines in four groups of patients with sepsis : Group 1, patients with Gram positive bacterial infection; Group 2, patients with Gram negative bacterial infection; Group 3, patients with negative cultures but strong evidence of infection; Group 4, patients without clinical and microbiologic evidence of infection as control group.

本研究主要以临床诊断为主再配合血液培养将病人分为四组：第一组是有革兰氏阳性细菌感染的病人；第二组是有革兰氏阴性细菌感染的病人；第三组是没有培养出细菌的感染性病人；第四组是由临床诊断及微生物培养证实为正常对照组。利用单株抗体接上萤光物质再配合流式细胞分析仪来测定淋巴球上各种细胞膜表面分化抗原的变化及利用酵素免疫分析法来探讨血清各种细胞素之变化。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

Both Mycoplana and Rothia can grow in the medium oil which is taken as only carbon source. Mycoplana is gram negative, fragmentates during early age. Rothia is gram positive and has obvious branching myceleum.

结果表明，这两种放线菌都能以变压器介质油为惟一碳源进行生长繁殖，前者为革兰氏染色阴性，培养早期断裂为杆状；后者为革兰氏染色阳性，有明显的分枝菌丝。

The best combination of isolating protoplast of Mozzie buster was the enzyme mixture containing 2.0% Cellulase R-10, 0.4% Pectinase, 0.5M mannitol as osmoticpressure, 27℃under dark 80r/m rotating enzymolysis 8h, and the highest yieldachieved to 1.67×10~6per gram, the protoplast vitality was 87.4%; The bestcombination of isolating protoplast of Ocimum basilicum was the enzyme mixturecontaining 1.6% Cellulase R-10, 0.4.% Pectinase, 0.5M mannitol as osmotic pressure,27℃under dark 80r/m rotating enzymolysis 8h, and the highest yield achieved to1.67×10~6 per gram, the protoplast vitality was 87.4%.

本研究对酶解最佳条件进行研究，得出分离驱蚊草原生质体酶解条件以CPW-13进行质壁分离1h，2.0％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压，27℃黑暗下80r／m酶解处理8h为好，产量可达1.67×10~6个／g，原生质体活力为87.4％；分离罗勒原生质体酶解条件以CPW-13进行质壁分离1h，1.6％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压为好，27℃黑暗下80r／m酶解处理8h，产量可达2.14×10~6个／g，原生质体活力为67.8％。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。