查询词典 gram method

Thermoanaerobacter tengcongensis is a rod-shaped, Gram-negative, anaerobic eubacterium that was isolated from a fresh water hot spring in Tengchong, China. Using a"whole-genome-shotgun"method, we sequenced its 2, 689, 445-bp genome from an isolate, MB4〓(Genbank Accession Number AE008691). The genome encodes 2, 588 predicted coding sequences . Among them, 1, 764 (68. 2％) are classified according to homology to other documented proteins, and the rest, 824 CDS (31. 8％), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86. 7％ of its genes are encoded on the leading strand of DNA replication.

中文题名腾冲嗜热厌氧菌全基因组测序及分析副题名外文题名 A complete sequence of the T.tengcongensis genome 论文作者包其郁导师杨焕明研究员学科专业遗传学研究领域\研究方向学位级别博士学位授予单位中国科学院遗传与发育生物学研究所学位授予日期2002 论文页码总数88页关键词腾冲嗜热厌氧菌基因组嗜热菌馆藏号BSLW /2003 /Q939.1 /33 腾冲嗜热厌氧菌（MB4＇）是分离于我国腾冲地区温泉内的革兰氏阴性厌氧真细菌。

Methods Acanthamoeba polyphaga and Legionella pneumophila were co-cultured under laboratory condition. At consecutive time points during the culture, smears of the cultured products were made on glass slides for staining purposes. Different types of stainings including Gram′s staining, Gimenez staining, Giemsa staining and immunofluorescence were used to determine the best method for the identification of amoebal pathogens.

方法嗜肺军团菌与多噬棘阿米巴共培养，取不同时点的共培养物制作涂片，采用革兰氏染色、吉曼尼兹染色、姬姆萨染色、免疫荧光染色等多种方法，用光学显微镜及荧光显微镜观察鉴别阿米巴滋养体与其胞内嗜肺军团菌，并比较这些染色方法的效果。

METHODS The minimal effective bactericidal concentration of 60 strains of Gram negative bacteria resistant to QACs was detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test, the qacE-sull gene of resistance to QACs and sulphonamide compounds by PCR.

方法使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物的最低有效杀菌浓度，利用改良的表型筛选试验检测耐QACs的表型，采用聚合酶链反应试验检测耐季铵盐消毒剂-磺胺复合物基因qacE-sul1。

Methods:The minimal effective bactericidal concentration of 60 strains Gram negative bacterium resistant to QACs were detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test,the qacE-sul1 gene of resistance to QACs and Sulphonamide Compou...

使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物的最低有效杀菌浓度，使用改良的表型筛选试验检测耐QACs的表型，使用聚合酶链反应试验检测耐季铵盐消毒剂-磺胺复合物基因qacE-sul1。

This sets question Israeli Baroque gram period of the whole music style characteristics Be a big background, concrete arrive the music style of the flute work, take this as to correspond a point, go deep into a concrete research Baroques period flute work to play a method.

此命题以巴洛克时期的整体音乐风格特点作为大背景，具体到长笛作品的音乐风格，以此为切入点，深入具体的研究巴洛克时期长笛作品的吹奏方法。

As endogenous jasmonates exist in enantiomers in nature and at level of nanogram/per gram fresh weight, it is very challenging work to develop rapid and accurate method for qualitative and quantitative analyses of JAs. This review paper summarizes the recent advances in the analysis of jasmonates including chromatography, capillary electrophoresis, chromatography-mass spectrometry and immunoassay.

内源茉莉酸类激素通常以多种手性异构体存在，且含量超微，因此，对内源性茉莉酸类激素进行准确的定性、定量分析具有很大的难度，研究高效分离富集、高灵敏度检测以及分离与检测联用等分析方法，对加速植物激素分子作用机理研究具有重大意义。

Results showed that the amount of tritium residual in the glass uranium was 2.68%, namely there was (0.0308±0.0003) mmol tritium per gram uranium. The standard deviation of PVT measurement method based on the state equation of the ideal gas was less than 0.95%, when the pressure was between 1500 and 133332 Pa.

直接测量法测量铀床的氚残留量的结果如下：铀床的氚残留量为2.68%，即每克铀含(0.0308±0.0003) mmol 氚气；当压力读数在1500～133332 Pa之间时，基于理想气体状态方程的测量方法的标准差小于0.95%。

The quantity of CD3+ cells in the EdNSC, AdNSC of cloning goattransplantation group and control group had no statistics difference(P＞0.05) at acute and chronic period.Overall, in our study, We got the conclusions as follow: 1,The EdNSC, AdNSCof cloning goat and the AdNSC of the non-cloning goat were successful obtained;EdNSC growed faster than AdNSC in the same cell culture method. 2,The braincontusion and laceration model of cloning goats were successful established by freefalling of 50 gram of steel ball accelerate strike on the goat brain from 100cm height.3, EdNSC and AdNSC transplantation both had obvious therapeutic effect on braincontusion and laceration of cloning goats; The therapeutic effect of EdNSCtransplantation was better. 4, Allograft of stem cells has acute and chronicimmunological rejection and autografting of stem cells has no acute and chronicimmunological rejection.

在相同的条件下，EdNSC体外培养速度比成AdNSC快。2对羊脑上薛氏沟周围运动皮层区使用50g×100 cm力度作自由落体加速撞击，可产生观察指标明确且存活率高的羊脑挫裂伤模型，有望广泛用于神经干细胞移植及药物等治疗的实验研究。3胚胎神经干细胞及成体神经干细胞移植治疗脑挫裂伤后均有较明显的治疗效果；二者相比较而言胚胎神经干细胞稍好于成体神经干细胞。4中枢神经系统异体神经干细胞移植存在着明显的免疫排斥反应，而自体神经干细胞移植基本无免疫排斥反应

Of bacteria being or relating to a bacterium that retains the violet stain used in Gram's method.

指在使用革兰氏方法时能保持所染的紫色的细菌，或者与这样的细菌相关。

Of bacteria) being or relating to a bacterium at retains the violet stain used in Gram 's method.

指在使用革兰氏方法时能保持所染的紫色的细菌，或者与这样的细菌相关。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失