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The histopathologic observations of corneas after penetrating keratoplasty in dogs: Thestructure of wholly transparent graft was the same as that of host cornea apart from the upperdonor-host junction where there was minimal irregularity of the corneal lamellae. However, thecorneal lamellae at the lower donor-host junction were neat. The graft with its central part beingtransparent only showed poor coaptation with recipient, in which the graft shifted inward and had marked wrinkles and folds in Descemet"s membrane. On the back of Descemet"s membranethere was hyperplastic stromal fibers that thickened the graft and partially adhered to iris.

5犬穿透性角膜移植术后角膜病理组织学观察完全透明的植片与植床结构一致,仅两者对合处上部纤维紊乱,而下方纤维排列整齐;中央透明的植片与植床对合不良,植片向植床方向嵌入,后弹力层皱缩、折叠,植片下方因有来自植床的基质纤维而增厚,部分对合处与虹膜粘连;浑浊的植片有比较完整的上皮层,植片约2/3深度的基质丧失原来纤维整齐排列结构,代以大量成纤维细胞增生。

For the PUU/BO-g-VER (n-butanol graft VER network)hybrid film, the miscibility between the soft and hard segments in PUU phase was improved byadding the BO-g-VER network into PUU, resulting in remarkable reinforcement effect andgreat improvements of mechanical properties for the films. In the PUU/200-g-VER [Mn: 200,poly graft VER], the 200-g-VER network had considerable compatibility withboth hard segments and soft segments in PUU phase, then good reinforcement effect wasrealized, giving rise to higher tensile strength and elastic module, lower elongation at break ofthe hybrid films.

由于BO-g-VER与PUU硬段具有较好的相容性且促进了PUU软硬段间的微相混合,导致PUU/BO-g-VER hybrid膜的力学性能有较大程度的提高,体现了明显的增强效果。200-g-VER(分子量为200的单官能团聚醚接枝VER)与PUU软硬段均有很好的相容性,因此PUU/200-g-VER hybrid有较好的增强效果,使胶膜的拉伸强度和弹性模量提高,断裂伸长率下降。

The effects of different factors on the laser-induced graft polymerization on the PET surface were analyzed, and the mechanisms were discussed. The factors were: 1 Monomers with smaller molecules resulted in better modification than polymeric monomers. Acrylamide monomer was chosen as hydrophilic modifier finally; 2 Higher concentration of the monomer solution favored the graft reaction; 3 With the increasing laser fluence, the surface hydrophilicity of PET films increased at first, then decreased. More laser pulses led to a more hydrophilic surface; 4 Benzophenone as a photosensitizer hindered the graft polymerization; 5 The homopolymer retardant of ammonium iron sulfate hexahydrate in a proper concentration retarded the formation of homopolymers and promotes the graft copolymerization; 6 The solvent of monomer solution also affected the graft reaction. Different solvents functioned in different ways. Ethanol can promote the graft reaction because of the activity of hydrogen in its structure; 7 pH value of the monomer solution affects the polymerization rate, and a lower pH value was favorable to the graft reaction.

这些影响因素的作用分别为:1小分子单体的表面改性效果好,确定以丙烯酰胺为亲水性改性单体;2较高的单体溶液浓度有利于接枝反应;3激光能量密度增大,薄膜表面亲水性先显著增强,后又减弱;激光脉冲次数越多,改性后表面亲水性越强;4光敏剂BP对PET表面接枝聚合反应有阻碍作用;5适当浓度的均聚物抑制剂六水合硫酸铁铵能减少单体均聚物的生成,促进接枝聚合;6单体溶液的溶剂也影响接枝聚合的反应进程,不同溶剂的影响作用原理不同,含较活泼氢的乙醇能促进接枝反应;7溶液pH值影响单体聚合速率,pH值较低有利于接枝反应。

Now the mechanism of action of heat shock protein is not quite sure to us.Understanding the biological function and its transplantation immunity of HSP will be helpful in investigating the relationship of HSP and graft ischemical reperfusion injury,graft rejection,HSP gene transfer,cardiac graft vascular disease.

目前,还不能确定热休克蛋白在上述反应过程中的直接作用的机制,了解热休克蛋白的生物学功能及其在移植免疫中的作用,有助于进行热休克蛋白与缺血/再灌注、移植排斥反应,以及热休克蛋白转基因治疗移植物血管病关系的研究。

Results The long term results of the vascularized fibular graft varied in different types of bone defects. Nine patients with congenital pseudoarthrosis of tibia had the limb function restored in 65% and one had nonunion of fibular graft, 3 fractures(7 times), and 5 angular deformities. Three patients with congenital radial deficiency had 62% restoration of the limb function. All of them showed early closure of epiphyses of the grafted fibulae. Five cases with osteomyelitis and bone defects combined soft tissue deficiency had 94% restoration of the limb function, and only one occurred fracture of fibular graft. One case with soft tissue defect and concomitant ulnar defect, ulnar nerve and median nerve injury, 67% function of limb was recovered, and union of fibula graft was good. 100% function of limb was recovered in 1 case with cavernoma of radius and without occurring valgus deformity of donor site.

结果应用带血管腓骨移植修复骨缺损的远期效果因缺损类型的不同而存在较大差异。9例先天性胫骨假关节患者,术后肢体功能恢复65%,其中移植腓骨骨不连1例、骨折3例(7次)、成角畸形5例;3例先天性桡骨缺如患者,术后肢体功能恢复62%,3例术后均出现移植腓骨骨骺线早闭,畸形复发;5例骨髓炎、骨外露、骨缺损并软组织缺损患者,术后肢体功能恢复94%,仅1例发生移植腓骨骨折;1例前臂软组织缺损并尺骨缺损,尺神经、正中神经损伤者,术后肢体功能恢复67%,骨愈合顺利;1例桡骨海绵状血管瘤者,术后肢体功能恢复100%,骨愈合顺利。

At the contralateral side, an ischiac bone graft (baseline 3 4 0.87) was transplanted. The healing period was 5 months. Group A11: The animals were treated in the same manner as in Group A5 with the difference that the healing period was 11 months. Group B5: The animals were treated in the same manner as in Group A5 with the difference that an e-PTFE membrane was adapted oer the bone graft on each side of the jaw.

愈合时间为5个月。A11组:用与A5组相同的方法处理小鼠,愈合时间为11个月。B5组:处理小鼠的方法与A5组相同,在每侧颌骨的骨移植物上方各置一片e-PTFE膜。B11组:实验方法与B5组相同,但在骨移植5月后,进行第二次手术取出生物膜,愈合时间为11个月。z在下颌骨骨量增加后,分别于第5个月处死A5组及B5组的小鼠,第11月处死A11组及B11组的小鼠,取下颌骨标本,体式显微镜测量骨移植物的宽度、长度及高度。

Objective: To establish the optimal height of the strut-graft for clinical surgery, this study analyzed the effect of change in strut-graft height on the lordotic curvatures and immediate biomechanical stability of the cervical spine on which corpectomy and strut-graft reconstruction with different height was performed.

目的:研究中下颈椎前路单椎体次全切除减压后不同撑开高度支撑植骨对颈椎曲度和颈椎即刻运动稳定性的影响,为临床手术确定合适的植骨高度提供参考。

objective to introduce a method for repair of defects of alae nasi.methods transplantation of whole or partial free auricle composite tissue was designed in reconstruction of alae nasi.partial auricle composite tissue transplantation was used in 14 patients,the area of the graft was 1.0cm×1.5cm~1.9cm×2.5cm.whole auricle composite tissue transplantation was used in 6 patients,the area of the graft was 0.5cm×0.5cm~0.8cm×1.2cm.results all grafts survived.the grafts were dark red or light red in color,there were no blisters on them.follow-up of more than six months showed good results.conclusion in many methods for repair of defects of alae nasi,this method is simple,the appearance of alae nasi is good after operation and there is no new scar on face.

目的 修复鼻翼缺损。方法采用全层或部分耳廓复合组织游离移植的方法修复鼻翼缺损20例,其中以鼻翼残存瘢痕组织翻转作衬里,然后进行部分耳廓复合组织游离移植14例,移植物面积最小1.0cm×1.5cm,最大1.9cm×2.5cm;利用耳廓复合组织全层嵌入游离移植修复鼻翼缺损者6例,移植物面积最小0.5cm×0.5cm,最大0.8cm×1.2cm。结果 20例移植组织全部成活。色泽呈暗红至淡红不等,无水泡。随访半年移植物色泽与鼻组织基本近似,供区隐蔽。结论在诸多鼻翼缺损修复方法中,该方法简便易行、术后鼻翼外形好,面部显著部位不增加新的瘢痕,有独到优越性。

Though it was slower in the frozen-thawed embryo transfer group than that of the fresh embryo transfer group, there were no significant differences in estrous cycle between experimental group and normal control group. The grafts were taken out and observed on day 63 after transplantation, rich blood vessels were formed on the cover of the graft, arterioles and venules could be seen under microscope. Histological examination of graft revealed that transplanted fetal ovary contained follicles in different developing stages, vesicula graafianae and corpus luteum were formed, and the morphous of graft was no obvious differences to normal ovaries.

冷冻移植组大鼠恢复动情周期虽稍短于新鲜移植组,但差异并无统计学意义。63 d后取出移植物观察,见移植物色泽红润,表面有丰富的血管形成,镜下见卵巢内有大量的小动脉和小静脉;移植物组织学检查发现卵泡的发育,光镜下可见不同发育阶段的卵泡,有成熟卵泡和黄体形成,形态与正常卵巢无明显差别。

The structure and graft rate of the graft polymer were determined by means of IR spectra and acid-base titrations, the effect of content of POE, MAH, DCP and reaction temperature on graft rate were studied in detail.

通过红外光谱确定了接枝物的结构,采用酸碱滴定法测定了产物的接枝率,详细研究了POE、MAH、DCP用量及反应温度等对接枝率的影响规律。

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