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The expressed protein, His-EGFP-CRP, was purified with affinity chromatography method and refolded with gradient filtration.

目的：表达纯化重组的His-EGFP-CRP蛋白，通过毛细管电泳技术对该重组蛋白的生物活性进行评价。

Therefore the denatured protein could pass along the gradient and refolded to native conformation as urea was gradually removed and a linear removal of urea was realized in this manner.

在与常用复性方法的比较中，这种方法在模型蛋白质溶菌酶的复性实验中初步显示出了一定的优越性。

The purity of the refolded rhIFN-γ was up to 90%, and high urea gradient length and moderate final urea concentration were favorable for the refolding process. Under optimum conditions, the specific activity of rhIFN-γ was up to 9.75×10^6IU/mg and protein recovery was up to 53.7%.

复性后的重组人γ-干扰素的纯度达90%；较长的脲梯度长度和合适的脲终浓度有利于蛋白质的复性；在较优的实验条件下，复性后的重组人γ-干扰素收率为53.7%，比活达9.75×10^6IU/mg。

The expressed protein, His-EGFP-CRP, was purified with affinity chromatography method and refolded with gradient filtration.

CRP在免疫反应过程中能识别病原体和宿主受损细胞，介导补体系统和吞噬细胞将它们清除，发挥免疫调理功能［2］。

In order to investigate the mechanism of urea gradient enhancing protein refolding, the renaturation kinetics of the two proteins was studied.

为了研究尿素浓度梯度促进蛋白质复性的机理，对两种目标蛋白质的复性动力学方程进行了研究。

This work mainly studies the application of MAXWELL fisheye micro-sphere-lens of virtual parameter with gradient refractive index distributing on dispersion material and the characteristic of MAXWELL fisheye lens with virtual parameter.

本工作研究的主要是具有梯度折射率(Gradient refractive index，GRIN)分布的虚宗量麦克斯韦鱼眼微球的光学性质、在表面漫射材料上的应用和虚宗量麦克斯韦鱼眼微球透镜的性质特点。

The total activity of EPs increased at first and then decreased during both senescences. The changes of endopeptidase isozymes in wheat leaf senescence were studied by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with hemoglobin or gelatin in the gel.

主要研究内容如下： 1、小麦叶片衰老过程中内肽酶的变化蛋白质的降解需要蛋白水解酶，包括内肽酶和外肽酶的参与，叶衰老过程中发挥主要作用的是内肽酶。

Six senescence-associated endopeptidase isoenzymes were identified by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with gelatin in the gel, five of which (EP1、EP2, EP4、EP5 and EP6) were only detected in senescing leaves.

另外，采用不同的内肽酶同工酶电泳方法研究中均发现自然衰老和暗诱导衰老过程中的内肽酶同工酶谱变化基本相似，因此暗诱导衰老可以作为一种研究叶片自然衰老过程中出现的蛋白水解酶的特性的模式。

Agilent ODS C_(18) column (250 mm×4.6 mm,5μm)was used,the acetoni- trile-water as mobile phase,gradient elution,the drift tube temperature was 70℃.

色谱柱Agilent ODS C_(18)柱(250 mm×4.6 mm，5μm)，流动相为乙腈-水，梯度洗脱，ELSD漂移管温度70℃；氮气流速2.0 L·min~(-1)；柱温35℃。

"Measure gradient at 5m intervals by using the abney level in combination with a supporting frame. For uneven slope s, measure at 1 m intervals."

用量角仪连同支架作为工具，沿样条每5米量度一次陡度；在陡度变化大的地区，可每米量度一次。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。